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Increased levels of NAD(P)H: quinone oxidoreductase 1 (NQO1) in pancreatic tissues from smokers and pancreatic adenocarcinomas: A potential biomarker of early damage in the pancreas.
Cell Biol Toxicol. 2006 Mar; 22(2):73-80.CB

Abstract

NAD(P)H:quinone oxidoreductase 1 (NQO1) is elevated in several human tumors. This study was conducted to determine whether increased levels of NQO1 expression also occur in human pancreatic tumor tissue, and to compare expression levels in nontumorous tissue from smokers with those in nonsmokers. The expression of NQO1 was examined in pancreatic tissue samples from 82 human donors. These samples included normal (n = 20), smokers (n = 25), pancreatitis (n = 7), and adenocarcinomas of the pancreas (n = 30). Genotyping for the C609T polymorphism in NQO1 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was also performed. Polymorphic variants were confirmed by automatic sequencing. Higher levels of NQO1 expression were demonstrated in pancreatic adenocarcinomas (0.831 +/- 0.021) compared to those in nontumorous tissues from nonsmokers (0.139 +/- 0.024). These high levels were also found in smokers (0.729 +/- 0.167) and in pancreatitis tissues (0.923 +/- 0.184). NQO1 activity was also higher in smokers (2.43 +/- 0.61 nmol/min per mg protein) compared to nonsmokers (0.44 +/- 0.05 nmol/min per mg protein; p < 0.05). No differences were found in genotype distribution and frequencies of the variant alleles between normal and cancer tissues in this relatively small sample pool. Seventy-five percent of the normal pancreatic tissues showed 609(C/C) and 25% 609(C/T). In pancreatic adenocarcinomas the frequency distribution was 65% C/C, 30% C/T and 5% T/T. The increased expression in noncancer pancreatic tissue from smokers and the fact that smoking is a moderate risk factor for pancreatic cancer suggest that NQO1 expression may be a good candidate as a biomarker for pancreatic cancer, especially in risk groups such as smokers.

Authors+Show Affiliations

Division of Molecular Epidemiology, National Center for Toxicological Research, Jefferson, Arizona, USA. blyncook@nctr.fda.govNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

16532285

Citation

Lyn-Cook, B D., et al. "Increased Levels of NAD(P)H: Quinone Oxidoreductase 1 (NQO1) in Pancreatic Tissues From Smokers and Pancreatic Adenocarcinomas: a Potential Biomarker of Early Damage in the Pancreas." Cell Biology and Toxicology, vol. 22, no. 2, 2006, pp. 73-80.
Lyn-Cook BD, Yan-Sanders Y, Moore S, et al. Increased levels of NAD(P)H: quinone oxidoreductase 1 (NQO1) in pancreatic tissues from smokers and pancreatic adenocarcinomas: A potential biomarker of early damage in the pancreas. Cell Biol Toxicol. 2006;22(2):73-80.
Lyn-Cook, B. D., Yan-Sanders, Y., Moore, S., Taylor, S., Word, B., & Hammons, G. J. (2006). Increased levels of NAD(P)H: quinone oxidoreductase 1 (NQO1) in pancreatic tissues from smokers and pancreatic adenocarcinomas: A potential biomarker of early damage in the pancreas. Cell Biology and Toxicology, 22(2), 73-80.
Lyn-Cook BD, et al. Increased Levels of NAD(P)H: Quinone Oxidoreductase 1 (NQO1) in Pancreatic Tissues From Smokers and Pancreatic Adenocarcinomas: a Potential Biomarker of Early Damage in the Pancreas. Cell Biol Toxicol. 2006;22(2):73-80. PubMed PMID: 16532285.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased levels of NAD(P)H: quinone oxidoreductase 1 (NQO1) in pancreatic tissues from smokers and pancreatic adenocarcinomas: A potential biomarker of early damage in the pancreas. AU - Lyn-Cook,B D, AU - Yan-Sanders,Y, AU - Moore,S, AU - Taylor,S, AU - Word,B, AU - Hammons,G J, Y1 - 2006/03/09/ PY - 2004/10/21/received PY - 2005/09/06/accepted PY - 2006/3/15/pubmed PY - 2006/12/9/medline PY - 2006/3/15/entrez SP - 73 EP - 80 JF - Cell biology and toxicology JO - Cell Biol Toxicol VL - 22 IS - 2 N2 - NAD(P)H:quinone oxidoreductase 1 (NQO1) is elevated in several human tumors. This study was conducted to determine whether increased levels of NQO1 expression also occur in human pancreatic tumor tissue, and to compare expression levels in nontumorous tissue from smokers with those in nonsmokers. The expression of NQO1 was examined in pancreatic tissue samples from 82 human donors. These samples included normal (n = 20), smokers (n = 25), pancreatitis (n = 7), and adenocarcinomas of the pancreas (n = 30). Genotyping for the C609T polymorphism in NQO1 by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was also performed. Polymorphic variants were confirmed by automatic sequencing. Higher levels of NQO1 expression were demonstrated in pancreatic adenocarcinomas (0.831 +/- 0.021) compared to those in nontumorous tissues from nonsmokers (0.139 +/- 0.024). These high levels were also found in smokers (0.729 +/- 0.167) and in pancreatitis tissues (0.923 +/- 0.184). NQO1 activity was also higher in smokers (2.43 +/- 0.61 nmol/min per mg protein) compared to nonsmokers (0.44 +/- 0.05 nmol/min per mg protein; p < 0.05). No differences were found in genotype distribution and frequencies of the variant alleles between normal and cancer tissues in this relatively small sample pool. Seventy-five percent of the normal pancreatic tissues showed 609(C/C) and 25% 609(C/T). In pancreatic adenocarcinomas the frequency distribution was 65% C/C, 30% C/T and 5% T/T. The increased expression in noncancer pancreatic tissue from smokers and the fact that smoking is a moderate risk factor for pancreatic cancer suggest that NQO1 expression may be a good candidate as a biomarker for pancreatic cancer, especially in risk groups such as smokers. SN - 0742-2091 UR - https://www.unboundmedicine.com/medline/citation/16532285/Increased_levels_of_NAD_P_H:_quinone_oxidoreductase_1__NQO1__in_pancreatic_tissues_from_smokers_and_pancreatic_adenocarcinomas:_A_potential_biomarker_of_early_damage_in_the_pancreas_ L2 - https://doi.org/10.1007/s10565-006-0156-3 DB - PRIME DP - Unbound Medicine ER -