Association between cigarette smoking and urinary excretion of 1,N2-ethenoguanine measured by isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry.
Chem Res Toxicol. 2005 Oct; 18(10):1593-9.CR

Abstract

Levels of the promutagenic 1,N2-ethenoguanine (1,N2-epsilonGua), an etheno DNA adduct derived mainly from lipid peroxidation, in experimental animals are associated with risk of cancer formation. Since 1,N2-epsilonGua can be repaired by human glycosylases, it is possible to use it as a biomarker for cancer risk assessment in humans. In the present study, a highly sensitive and specific stable isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-ESI/MS/MS) was developed for accurate quantification of 1,N2-epsilonGua in human urine. The sample pretreatment involved a consecutive strong cation exchange solid-phase extraction (SPE) and reversed phase SPE chromatography. The pretreated sample was analyzed by LC-ESI/MS/MS under multiple reaction monitoring mode (MRM) using a triple quadrupole mass spectrometer. The detection limit of 1,N2-epsilonGua using this LC-ESI/MS/MS assay was 1.0 pg (5.8 fmol) injected on-column. Levels of urine samples collected from healthy volunteers were found to range from 0 to 199 pg/mL, and levels as low as 5.0 pg/mL (29 pM) could be accurately quantified. After adjusting for creatinine levels and body weight, an statistically significant association was observed between urinary levels of 1,N2-epsilonGua and cigarette smoking (p = 0.0006). This highly specific and sensitive assay should be valuable in measuring urinary 1,N2-epsilonGua as a potential noninvasive biomarker for oxidative DNA damage.

Authors+Show Affiliations

Chen HJ

Department of Chemistry and Biochemistry, National Chung Cheng University, 168 University Road, Ming-Hsiung, Chia-Yi 62142, Taiwan. chehjc@ccu.edu.tw

Chiu WL

No affiliation info available

MeSH

AdultBiomarkersChromatography, LiquidDNA AdductsFemaleGuanineHumansIndicator Dilution TechniquesMaleMiddle AgedReproducibility of ResultsSmokingSpectrometry, Mass, Electrospray Ionization

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16533024

Citation

Chen, Hauh-Jyun Candy, and Wei-Loong Chiu. "Association Between Cigarette Smoking and Urinary Excretion of 1,N2-ethenoguanine Measured By Isotope Dilution Liquid Chromatography-electrospray Ionization/tandem Mass Spectrometry." Chemical Research in Toxicology, vol. 18, no. 10, 2005, pp. 1593-9.

Chen HJ, Chiu WL. Association between cigarette smoking and urinary excretion of 1,N2-ethenoguanine measured by isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry. Chem Res Toxicol. 2005;18(10):1593-9.

Chen, H. J., & Chiu, W. L. (2005). Association between cigarette smoking and urinary excretion of 1,N2-ethenoguanine measured by isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry. Chemical Research in Toxicology, 18(10), 1593-9.

Chen HJ, Chiu WL. Association Between Cigarette Smoking and Urinary Excretion of 1,N2-ethenoguanine Measured By Isotope Dilution Liquid Chromatography-electrospray Ionization/tandem Mass Spectrometry. Chem Res Toxicol. 2005;18(10):1593-9. PubMed PMID: 16533024.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Association between cigarette smoking and urinary excretion of 1,N2-ethenoguanine measured by isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry. AU - Chen,Hauh-Jyun Candy, AU - Chiu,Wei-Loong, PY - 2006/3/15/pubmed PY - 2007/4/25/medline PY - 2006/3/15/entrez SP - 1593 EP - 9 JF - Chemical research in toxicology JO - Chem Res Toxicol VL - 18 IS - 10 N2 - Levels of the promutagenic 1,N2-ethenoguanine (1,N2-epsilonGua), an etheno DNA adduct derived mainly from lipid peroxidation, in experimental animals are associated with risk of cancer formation. Since 1,N2-epsilonGua can be repaired by human glycosylases, it is possible to use it as a biomarker for cancer risk assessment in humans. In the present study, a highly sensitive and specific stable isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-ESI/MS/MS) was developed for accurate quantification of 1,N2-epsilonGua in human urine. The sample pretreatment involved a consecutive strong cation exchange solid-phase extraction (SPE) and reversed phase SPE chromatography. The pretreated sample was analyzed by LC-ESI/MS/MS under multiple reaction monitoring mode (MRM) using a triple quadrupole mass spectrometer. The detection limit of 1,N2-epsilonGua using this LC-ESI/MS/MS assay was 1.0 pg (5.8 fmol) injected on-column. Levels of urine samples collected from healthy volunteers were found to range from 0 to 199 pg/mL, and levels as low as 5.0 pg/mL (29 pM) could be accurately quantified. After adjusting for creatinine levels and body weight, an statistically significant association was observed between urinary levels of 1,N2-epsilonGua and cigarette smoking (p = 0.0006). This highly specific and sensitive assay should be valuable in measuring urinary 1,N2-epsilonGua as a potential noninvasive biomarker for oxidative DNA damage. SN - 0893-228X UR - https://www.unboundmedicine.com/medline/citation/16533024/Association_between_cigarette_smoking_and_urinary_excretion_of_1N2_ethenoguanine_measured_by_isotope_dilution_liquid_chromatography_electrospray_ionization/tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -

Tags

Association between cigarette smoking and urinary excretion of 1,N2-ethenoguanine measured by isotope dilution liquid chromatography-electrospray ionization/tandem mass spectrometry.Chem Res Toxicol. 2005 Oct; 18(10):1593-9.CR