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Resistance of strains producing extended-spectrum beta-lactamases and genotype distribution in China.
J Infect. 2007 Jan; 54(1):53-7.JI

Abstract

OBJECTIVE

To investigate the resistance of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum beta-lactamases (ESBLs) and the genotyping of ESBLs in China.

METHODS

MICs of 12 antibiotics against 50 strains (by random selection) of ESBLs-producing E. coli and K. pneumoniae were determined by E-test. The genotypes of ESBLs were analyzed by PCR, DNA sequencing and isoelectric focusing.

RESULTS

The susceptibility rate of 50 isolates was 100% in imipenem, 60%-80% in cefoperazone/sulbactam, ceftazidime and piperacillin/tazobactam, and lower in other antimicrobial agents tested. Only 6.0% of the isolates were sensitive to cefotaxime. Four hundred and forty-seven of 509 isolates had been confirmed the genotype of ESBLs. Four hundred and sixteen strains produced only one type of ESBLs, including CTX-M-14 (271 strains), CTX-M-3 (70 strains), CTX-M-24 (35 strains), CTX-M-22 (8 strains), CTX-M-15 (4 strains), CTX-M-9 (4 strains), CTX-M-28 (3 strains), CTX-M-12 (1 strain), CTX-M-13 (1 strain), CTX-M-27 (1 strain), CTX-M-29 (1 strain), SHV-12 (10 strains), SHV-5 (4 strains), SHV-2 (2 strains), and SHV-9 (1 strain). Thirty isolates carried two or three types of ESBLs, and producing CTX-M-14 and CTX-M-3 together were the most common type.

CONCLUSION

The resistance of E. coli and K. pneumonia producing ESBLs in China was a serious issue and CTX-M type ESBLs were the most common genotype. CTX-M-14 was the predominant genotype. Some isolates produced two or three ESBLs.

Authors+Show Affiliations

Department of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. yvys119@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16533535

Citation

Yu, Yunsong, et al. "Resistance of Strains Producing Extended-spectrum Beta-lactamases and Genotype Distribution in China." The Journal of Infection, vol. 54, no. 1, 2007, pp. 53-7.
Yu Y, Ji S, Chen Y, et al. Resistance of strains producing extended-spectrum beta-lactamases and genotype distribution in China. J Infect. 2007;54(1):53-7.
Yu, Y., Ji, S., Chen, Y., Zhou, W., Wei, Z., Li, L., & Ma, Y. (2007). Resistance of strains producing extended-spectrum beta-lactamases and genotype distribution in China. The Journal of Infection, 54(1), 53-7.
Yu Y, et al. Resistance of Strains Producing Extended-spectrum Beta-lactamases and Genotype Distribution in China. J Infect. 2007;54(1):53-7. PubMed PMID: 16533535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resistance of strains producing extended-spectrum beta-lactamases and genotype distribution in China. AU - Yu,Yunsong, AU - Ji,Shujuan, AU - Chen,Yagang, AU - Zhou,Weilin, AU - Wei,Zeqing, AU - Li,Lanjuan, AU - Ma,Yilin, Y1 - 2006/03/13/ PY - 2005/03/29/received PY - 2006/01/22/accepted PY - 2006/3/15/pubmed PY - 2007/3/3/medline PY - 2006/3/15/entrez SP - 53 EP - 7 JF - The Journal of infection JO - J Infect VL - 54 IS - 1 N2 - OBJECTIVE: To investigate the resistance of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum beta-lactamases (ESBLs) and the genotyping of ESBLs in China. METHODS: MICs of 12 antibiotics against 50 strains (by random selection) of ESBLs-producing E. coli and K. pneumoniae were determined by E-test. The genotypes of ESBLs were analyzed by PCR, DNA sequencing and isoelectric focusing. RESULTS: The susceptibility rate of 50 isolates was 100% in imipenem, 60%-80% in cefoperazone/sulbactam, ceftazidime and piperacillin/tazobactam, and lower in other antimicrobial agents tested. Only 6.0% of the isolates were sensitive to cefotaxime. Four hundred and forty-seven of 509 isolates had been confirmed the genotype of ESBLs. Four hundred and sixteen strains produced only one type of ESBLs, including CTX-M-14 (271 strains), CTX-M-3 (70 strains), CTX-M-24 (35 strains), CTX-M-22 (8 strains), CTX-M-15 (4 strains), CTX-M-9 (4 strains), CTX-M-28 (3 strains), CTX-M-12 (1 strain), CTX-M-13 (1 strain), CTX-M-27 (1 strain), CTX-M-29 (1 strain), SHV-12 (10 strains), SHV-5 (4 strains), SHV-2 (2 strains), and SHV-9 (1 strain). Thirty isolates carried two or three types of ESBLs, and producing CTX-M-14 and CTX-M-3 together were the most common type. CONCLUSION: The resistance of E. coli and K. pneumonia producing ESBLs in China was a serious issue and CTX-M type ESBLs were the most common genotype. CTX-M-14 was the predominant genotype. Some isolates produced two or three ESBLs. SN - 1532-2742 UR - https://www.unboundmedicine.com/medline/citation/16533535/Resistance_of_strains_producing_extended_spectrum_beta_lactamases_and_genotype_distribution_in_China_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0163-4453(06)00027-2 DB - PRIME DP - Unbound Medicine ER -