Tags

Type your tag names separated by a space and hit enter

NPY modulates neurotransmission of CGRP-containing vasodilator nerves in rat mesenteric arteries.
Am J Physiol. 1991 Sep; 261(3 Pt 2):H683-90.AJ

Abstract

The effect of neuropeptide Y (NPY) in neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves was investigated in rats. In perfused mesenteric vascular beds with active tone, perivascular nerve stimulation (PNS; 1-8 Hz) caused a frequency-dependent vasodilator response, which was abolished by 300 nM tetrodotoxin (TTX), 500 nM capsaicin, 1 microM human CGRP-(8-37), or cold storage denervation (4 degrees C for 72 h). NPY (5, 10, and 50 nM) concentration dependently inhibited the vasodilator response to PNS, whereas NPY had little effect on vasodilation induced by exogenous CGRP (10 and 100 pmol) or 1 nmol acetylcholine (ACh). NPY (10 nM) inhibited the neurogenic release of CGRP-like immunoreactivity induced by PNS (4 and 8 Hz), which was abolished by 300 nM TTX and the removal of Ca2+ from the medium. Combined perfusion with 5 nM NPY and 10 nM norepinephrine additively inhibited the vasodilator response to PNS but not to exogenous CGRP and ACh. Immunohistochemistry showed the distinct distribution of CGRP- and NPY-like immunoreactivity-containing fibers in rat mesenteric arteries. These results suggest that NPY modulates presynaptically the release of CGRP from CGRP-containing vasodilator nerves in rat mesenteric arteries.

Authors+Show Affiliations

Department of Pharmacology, Miyazaki Medical College, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1653537

Citation

Kawasaki, H, et al. "NPY Modulates Neurotransmission of CGRP-containing Vasodilator Nerves in Rat Mesenteric Arteries." The American Journal of Physiology, vol. 261, no. 3 Pt 2, 1991, pp. H683-90.
Kawasaki H, Nuki C, Saito A, et al. NPY modulates neurotransmission of CGRP-containing vasodilator nerves in rat mesenteric arteries. Am J Physiol. 1991;261(3 Pt 2):H683-90.
Kawasaki, H., Nuki, C., Saito, A., & Takasaki, K. (1991). NPY modulates neurotransmission of CGRP-containing vasodilator nerves in rat mesenteric arteries. The American Journal of Physiology, 261(3 Pt 2), H683-90.
Kawasaki H, et al. NPY Modulates Neurotransmission of CGRP-containing Vasodilator Nerves in Rat Mesenteric Arteries. Am J Physiol. 1991;261(3 Pt 2):H683-90. PubMed PMID: 1653537.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NPY modulates neurotransmission of CGRP-containing vasodilator nerves in rat mesenteric arteries. AU - Kawasaki,H, AU - Nuki,C, AU - Saito,A, AU - Takasaki,K, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - H683 EP - 90 JF - The American journal of physiology JO - Am J Physiol VL - 261 IS - 3 Pt 2 N2 - The effect of neuropeptide Y (NPY) in neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves was investigated in rats. In perfused mesenteric vascular beds with active tone, perivascular nerve stimulation (PNS; 1-8 Hz) caused a frequency-dependent vasodilator response, which was abolished by 300 nM tetrodotoxin (TTX), 500 nM capsaicin, 1 microM human CGRP-(8-37), or cold storage denervation (4 degrees C for 72 h). NPY (5, 10, and 50 nM) concentration dependently inhibited the vasodilator response to PNS, whereas NPY had little effect on vasodilation induced by exogenous CGRP (10 and 100 pmol) or 1 nmol acetylcholine (ACh). NPY (10 nM) inhibited the neurogenic release of CGRP-like immunoreactivity induced by PNS (4 and 8 Hz), which was abolished by 300 nM TTX and the removal of Ca2+ from the medium. Combined perfusion with 5 nM NPY and 10 nM norepinephrine additively inhibited the vasodilator response to PNS but not to exogenous CGRP and ACh. Immunohistochemistry showed the distinct distribution of CGRP- and NPY-like immunoreactivity-containing fibers in rat mesenteric arteries. These results suggest that NPY modulates presynaptically the release of CGRP from CGRP-containing vasodilator nerves in rat mesenteric arteries. SN - 0002-9513 UR - https://www.unboundmedicine.com/medline/citation/1653537/NPY_modulates_neurotransmission_of_CGRP_containing_vasodilator_nerves_in_rat_mesenteric_arteries_ L2 - https://journals.physiology.org/doi/10.1152/ajpheart.1991.261.3.H683?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -