Tags

Type your tag names separated by a space and hit enter

Hemicelluloses of ragi (finger millet, Eleusine coracana, Indaf-15): isolation and purification of an alkali-extractable arabinoxylan from native and malted hemicellulose B.
J Agric Food Chem. 2006 Mar 22; 54(6):2342-9.JA

Abstract

Hemicelluloses (A and B) were isolated from an Indo-African hybrid variety of finger millet (ragi, Eleusine coracana) by extracting the starch-free residue with 10% sodium hydroxide under a continuous stream of nitrogen, and changes in their sugar composition during malting for 96 h were studied. Hemicellulose B, obtained in higher yield from both native (N) and malted (M) flours, was found to be completely soluble in water, richer in uronic acid, and more viscogenic than hemicelullose A. Fractional precipitation of hemicellulose B by ammonium sulfate resulted in four precipitable fractions (F-60, F-70, F-80, and F-100) and a nonprecipitable (NP) fraction varying in their yield and arabinose, xylose, galactose, and glucose contents. A progressive increase in the pentose-to-hexose ratio (P:H) from 0.42:1.0 in F-60 to 1.94:1.0 in NP was observed in native hemicellulose B fractions; however, in malted hemicellulose B the P:H ratio increased from 0.43:1.0 in F-60 to 1.56:1.0 in F-80 and then decreased to 1.13:1.0 in NP. The major fraction, F-70 (N, 44.5%; M, 38.5%), was separated into eight subfractions on DEAE-cellulose by successive elution with water, ammonium carbonate (AC) (0.1, 0.2, and 0.3 M AC), and sodium hydroxide (0.1 and 0.2 M) differing in their yield and neutral sugar composition. The purity of the major glucuronoarabinoxylan fraction (0.1 M AC eluted) was ascertained by Sepharose CL-4B, HPSEC, cellulose acetate, and capillary electrophoresis methods. A significant decrease in the molecular mass of arabinoxylan from 1200 to 1120 kDa upon malting for 96 h is an indication of cell wall degradation by the inducible cell wall degrading enzymes.

Authors+Show Affiliations

Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, Karnataka 570 020, India.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16536617

Citation

Subba Rao, M V S S T., and G Muralikrishna. "Hemicelluloses of Ragi (finger Millet, Eleusine Coracana, Indaf-15): Isolation and Purification of an Alkali-extractable Arabinoxylan From Native and Malted Hemicellulose B." Journal of Agricultural and Food Chemistry, vol. 54, no. 6, 2006, pp. 2342-9.
Subba Rao MV, Muralikrishna G. Hemicelluloses of ragi (finger millet, Eleusine coracana, Indaf-15): isolation and purification of an alkali-extractable arabinoxylan from native and malted hemicellulose B. J Agric Food Chem. 2006;54(6):2342-9.
Subba Rao, M. V., & Muralikrishna, G. (2006). Hemicelluloses of ragi (finger millet, Eleusine coracana, Indaf-15): isolation and purification of an alkali-extractable arabinoxylan from native and malted hemicellulose B. Journal of Agricultural and Food Chemistry, 54(6), 2342-9.
Subba Rao MV, Muralikrishna G. Hemicelluloses of Ragi (finger Millet, Eleusine Coracana, Indaf-15): Isolation and Purification of an Alkali-extractable Arabinoxylan From Native and Malted Hemicellulose B. J Agric Food Chem. 2006 Mar 22;54(6):2342-9. PubMed PMID: 16536617.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hemicelluloses of ragi (finger millet, Eleusine coracana, Indaf-15): isolation and purification of an alkali-extractable arabinoxylan from native and malted hemicellulose B. AU - Subba Rao,M V S S T, AU - Muralikrishna,G, PY - 2006/3/16/pubmed PY - 2006/4/20/medline PY - 2006/3/16/entrez SP - 2342 EP - 9 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 54 IS - 6 N2 - Hemicelluloses (A and B) were isolated from an Indo-African hybrid variety of finger millet (ragi, Eleusine coracana) by extracting the starch-free residue with 10% sodium hydroxide under a continuous stream of nitrogen, and changes in their sugar composition during malting for 96 h were studied. Hemicellulose B, obtained in higher yield from both native (N) and malted (M) flours, was found to be completely soluble in water, richer in uronic acid, and more viscogenic than hemicelullose A. Fractional precipitation of hemicellulose B by ammonium sulfate resulted in four precipitable fractions (F-60, F-70, F-80, and F-100) and a nonprecipitable (NP) fraction varying in their yield and arabinose, xylose, galactose, and glucose contents. A progressive increase in the pentose-to-hexose ratio (P:H) from 0.42:1.0 in F-60 to 1.94:1.0 in NP was observed in native hemicellulose B fractions; however, in malted hemicellulose B the P:H ratio increased from 0.43:1.0 in F-60 to 1.56:1.0 in F-80 and then decreased to 1.13:1.0 in NP. The major fraction, F-70 (N, 44.5%; M, 38.5%), was separated into eight subfractions on DEAE-cellulose by successive elution with water, ammonium carbonate (AC) (0.1, 0.2, and 0.3 M AC), and sodium hydroxide (0.1 and 0.2 M) differing in their yield and neutral sugar composition. The purity of the major glucuronoarabinoxylan fraction (0.1 M AC eluted) was ascertained by Sepharose CL-4B, HPSEC, cellulose acetate, and capillary electrophoresis methods. A significant decrease in the molecular mass of arabinoxylan from 1200 to 1120 kDa upon malting for 96 h is an indication of cell wall degradation by the inducible cell wall degrading enzymes. SN - 0021-8561 UR - https://www.unboundmedicine.com/medline/citation/16536617/Hemicelluloses_of_ragi__finger_millet_Eleusine_coracana_Indaf_15_:_isolation_and_purification_of_an_alkali_extractable_arabinoxylan_from_native_and_malted_hemicellulose_B_ L2 - https://doi.org/10.1021/jf058144q DB - PRIME DP - Unbound Medicine ER -