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An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium).
Artif Cells Blood Substit Immobil Biotechnol. 2006; 34(2):223-39.AC

Abstract

A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and bovine serum albumin (BSA) was selected as the best immobilization method for the enzymatic system. Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at + 0.7 V vs. Ag/AgCl. The linear working range of the electrode was 2.0 x 10(-5) - 3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The stability and reproducibility of the enzyme electrode have been also studied.

Authors+Show Affiliations

Erden PE
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
Pekyardimci S
No affiliation info available
Kiliç E
No affiliation info available
Arslan F
No affiliation info available

MeSH

Biosensing TechniquesConductometryCreatineCross-Linking ReagentsEnzymes, ImmobilizedFerrous CompoundsGlutaralHumansHydrogen-Ion ConcentrationOxidation-ReductionPhosphatesPolyvinylsSarcosine OxidaseSerum Albumin, BovineTemperatureUreohydrolases

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16537176

Citation

Erden, Pinar Esra, et al. "An Amperometric Enzyme Electrode for Creatine Determination Prepared By the Immobilization of Creatinase and Sarcosine Oxidase in Poly(vinylferrocenium)." Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, vol. 34, no. 2, 2006, pp. 223-39.
Erden PE, Pekyardimci S, Kiliç E, et al. An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium). Artif Cells Blood Substit Immobil Biotechnol. 2006;34(2):223-39.
Erden, P. E., Pekyardimci, S., Kiliç, E., & Arslan, F. (2006). An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium). Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, 34(2), 223-39.
Erden PE, et al. An Amperometric Enzyme Electrode for Creatine Determination Prepared By the Immobilization of Creatinase and Sarcosine Oxidase in Poly(vinylferrocenium). Artif Cells Blood Substit Immobil Biotechnol. 2006;34(2):223-39. PubMed PMID: 16537176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An amperometric enzyme electrode for creatine determination prepared by the immobilization of creatinase and sarcosine oxidase in poly(vinylferrocenium). AU - Erden,Pinar Esra, AU - Pekyardimci,Sule, AU - Kiliç,Esma, AU - Arslan,Fatma, PY - 2006/3/16/pubmed PY - 2006/6/10/medline PY - 2006/3/16/entrez SP - 223 EP - 39 JF - Artificial cells, blood substitutes, and immobilization biotechnology JO - Artif Cells Blood Substit Immobil Biotechnol VL - 34 IS - 2 N2 - A new enzyme electrode for the determination of creatine was developed by immobilizing creatinase (CI) and sarcosine oxidase (SO). The enzymes were co-immobilized in a poly(vinylferrocenium) matrix onto the surface of a platinum working electrode. Crosslinking with glutaraldehyte (GA) and bovine serum albumin (BSA) was selected as the best immobilization method for the enzymatic system. Determination of creatine was performed by the oxidation of enzymatically generated H2O2 at + 0.7 V vs. Ag/AgCl. The linear working range of the electrode was 2.0 x 10(-5) - 3.2 x 10(-4) M and the response time was about 50 s. The effects of pH, temperature, enzyme ratio and buffer concentration were investigated and optimum parameters were found to be 7.5, 37 degrees C, 2.5:1 (CI:SO) and 0.05 M, respectively. The stability and reproducibility of the enzyme electrode have been also studied. SN - 1073-1199 UR - https://www.unboundmedicine.com/medline/citation/16537176/An_amperometric_enzyme_electrode_for_creatine_determination_prepared_by_the_immobilization_of_creatinase_and_sarcosine_oxidase_in_poly_vinylferrocenium__ DB - PRIME DP - Unbound Medicine ER -