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Inhibitory effects of caraway (Carum carvi L.) and its component on N-methyl-N'-nitro-N-nitrosoguanidine-induced mutagenicity.
J Med Invest. 2006 Feb; 53(1-2):123-33.JM

Abstract

To elucidate the mechanism of antimutagenicity of caraway, we examined the effects of caraway seed extract on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutagenesis in DNA methyltransferase-deficient Salmonella typhimurium strains, O6-methylguanine DNA adduct formation, and thiol content in S. typhimurium cells. MNNG was highly mutagenic for ogt- strains YG7104 (ogt- ada+) and YG7108 (ogt- ada-), and it showed slightly higher mutagenicity in strain YG7100 (ogt+ ada-) than in strains TA100 and TA1535. Hot water extract of caraway seeds inhibited MNNG-induced mutation only in the ogt+ strains. In the presence of caraway extract, O6-methylguanine DNA adducts in strain YG7100 were decreased in proportion to the decrease of MNNG-induced mutagenesis. Although MNNG is known to degrade in the presence of thiols to produce methyl cation which can react with DNA, caraway had no effect on cellular concentrations of acid-soluble thiols. These results indicate that caraway does not directly inactivate MNNG and that Ogt-O6-methylguanine-DNA methyltransferase may be involved in the antimutagenic activity of caraway.

Authors+Show Affiliations

Mazaki M
Department of Molecular Bacteriology, Institute of Health Biosciences, The University of Tokushima Graduate School, Japan.
Kataoka K
No affiliation info available
Kinouchi T
No affiliation info available
Vinitketkumnuen U
No affiliation info available
Yamada M
No affiliation info available
Nohmi T
No affiliation info available
Kuwahara T
No affiliation info available
Akimoto S
No affiliation info available
Ohnishi Y
No affiliation info available

MeSH

Antimutagenic AgentsCarumDNA AdductsMethylnitronitrosoguanidineMutagenicity TestsMutagensPlant ExtractsSalmonella typhimuriumSeedsSulfhydryl Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16538005

Citation

Mazaki, Masanori, et al. "Inhibitory Effects of Caraway (Carum Carvi L.) and Its Component On N-methyl-N'-nitro-N-nitrosoguanidine-induced Mutagenicity." The Journal of Medical Investigation : JMI, vol. 53, no. 1-2, 2006, pp. 123-33.
Mazaki M, Kataoka K, Kinouchi T, et al. Inhibitory effects of caraway (Carum carvi L.) and its component on N-methyl-N'-nitro-N-nitrosoguanidine-induced mutagenicity. J Med Invest. 2006;53(1-2):123-33.
Mazaki, M., Kataoka, K., Kinouchi, T., Vinitketkumnuen, U., Yamada, M., Nohmi, T., Kuwahara, T., Akimoto, S., & Ohnishi, Y. (2006). Inhibitory effects of caraway (Carum carvi L.) and its component on N-methyl-N'-nitro-N-nitrosoguanidine-induced mutagenicity. The Journal of Medical Investigation : JMI, 53(1-2), 123-33.
Mazaki M, et al. Inhibitory Effects of Caraway (Carum Carvi L.) and Its Component On N-methyl-N'-nitro-N-nitrosoguanidine-induced Mutagenicity. J Med Invest. 2006;53(1-2):123-33. PubMed PMID: 16538005.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effects of caraway (Carum carvi L.) and its component on N-methyl-N'-nitro-N-nitrosoguanidine-induced mutagenicity. AU - Mazaki,Masanori, AU - Kataoka,Keiko, AU - Kinouchi,Takemi, AU - Vinitketkumnuen,Usanee, AU - Yamada,Masami, AU - Nohmi,Takehiko, AU - Kuwahara,Tomomi, AU - Akimoto,Shigeru, AU - Ohnishi,Yoshinari, PY - 2006/3/16/pubmed PY - 2006/5/26/medline PY - 2006/3/16/entrez SP - 123 EP - 33 JF - The journal of medical investigation : JMI JO - J Med Invest VL - 53 IS - 1-2 N2 - To elucidate the mechanism of antimutagenicity of caraway, we examined the effects of caraway seed extract on N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced mutagenesis in DNA methyltransferase-deficient Salmonella typhimurium strains, O6-methylguanine DNA adduct formation, and thiol content in S. typhimurium cells. MNNG was highly mutagenic for ogt- strains YG7104 (ogt- ada+) and YG7108 (ogt- ada-), and it showed slightly higher mutagenicity in strain YG7100 (ogt+ ada-) than in strains TA100 and TA1535. Hot water extract of caraway seeds inhibited MNNG-induced mutation only in the ogt+ strains. In the presence of caraway extract, O6-methylguanine DNA adducts in strain YG7100 were decreased in proportion to the decrease of MNNG-induced mutagenesis. Although MNNG is known to degrade in the presence of thiols to produce methyl cation which can react with DNA, caraway had no effect on cellular concentrations of acid-soluble thiols. These results indicate that caraway does not directly inactivate MNNG and that Ogt-O6-methylguanine-DNA methyltransferase may be involved in the antimutagenic activity of caraway. SN - 1343-1420 UR - https://www.unboundmedicine.com/medline/citation/16538005/Inhibitory_effects_of_caraway__Carum_carvi_L___and_its_component_on_N_methyl_N'_nitro_N_nitrosoguanidine_induced_mutagenicity_ DB - PRIME DP - Unbound Medicine ER -