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Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide.
Biochem Biophys Res Commun. 2006 Apr 28; 343(1):303-10.BB

Abstract

The gaseous mediators hydrogen sulphide (H2S) and nitric oxide (*NO) are synthesised in the body from L-cysteine and L-arginine, respectively. In the cardiovascular system, *NO is an important regulator of vascular tone and its over- or under-production has been linked to a variety of diseases. The physiological significance of H2S is not yet clear but, like *NO, it exhibits vasodilator activity and may play a part in septic and haemorrhagic shock, hypertension, regulation of cardiac contractility, and in inflammation. To date, there have been no reports of a chemical interaction between H2S and *NO. Here we show that incubation of the H2S donor, sodium hydrosulphide, with a range of *NO donors and *NO gas in vitro leads to the formation of a nitrosothiol molecule as determined by a combination of techniques; electron paramagnetic resonance, amperometry, and measurement of nitrite. We further show that this nitrosothiol did not induce cGMP accumulation in cultured RAW264.7 cells unless *NO was released with Cu2+. Finally, using liver homogenates from LPS treated rats we present evidence for the endogenous formation of this nitrosothiol. These findings provide the first evidence for the formation of a novel nitrosothiol generated by reaction between H2S and *NO. We propose that generation of this nitrosothiol in the body may regulate the physiological effects of both *NO and H2S.

Authors+Show Affiliations

Cardiovascular Biology Research Group, Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Republic of Singapore 117597. bchwm1@nus.edu.sgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16540095

Citation

Whiteman, Matthew, et al. "Evidence for the Formation of a Novel Nitrosothiol From the Gaseous Mediators Nitric Oxide and Hydrogen Sulphide." Biochemical and Biophysical Research Communications, vol. 343, no. 1, 2006, pp. 303-10.
Whiteman M, Li L, Kostetski I, et al. Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide. Biochem Biophys Res Commun. 2006;343(1):303-10.
Whiteman, M., Li, L., Kostetski, I., Chu, S. H., Siau, J. L., Bhatia, M., & Moore, P. K. (2006). Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide. Biochemical and Biophysical Research Communications, 343(1), 303-10.
Whiteman M, et al. Evidence for the Formation of a Novel Nitrosothiol From the Gaseous Mediators Nitric Oxide and Hydrogen Sulphide. Biochem Biophys Res Commun. 2006 Apr 28;343(1):303-10. PubMed PMID: 16540095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide. AU - Whiteman,Matthew, AU - Li,Ling, AU - Kostetski,Iouri, AU - Chu,Siew Hwa, AU - Siau,Jia Ling, AU - Bhatia,Madhav, AU - Moore,Philip K, Y1 - 2006/03/06/ PY - 2006/02/23/received PY - 2006/02/24/accepted PY - 2006/3/17/pubmed PY - 2006/5/25/medline PY - 2006/3/17/entrez SP - 303 EP - 10 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 343 IS - 1 N2 - The gaseous mediators hydrogen sulphide (H2S) and nitric oxide (*NO) are synthesised in the body from L-cysteine and L-arginine, respectively. In the cardiovascular system, *NO is an important regulator of vascular tone and its over- or under-production has been linked to a variety of diseases. The physiological significance of H2S is not yet clear but, like *NO, it exhibits vasodilator activity and may play a part in septic and haemorrhagic shock, hypertension, regulation of cardiac contractility, and in inflammation. To date, there have been no reports of a chemical interaction between H2S and *NO. Here we show that incubation of the H2S donor, sodium hydrosulphide, with a range of *NO donors and *NO gas in vitro leads to the formation of a nitrosothiol molecule as determined by a combination of techniques; electron paramagnetic resonance, amperometry, and measurement of nitrite. We further show that this nitrosothiol did not induce cGMP accumulation in cultured RAW264.7 cells unless *NO was released with Cu2+. Finally, using liver homogenates from LPS treated rats we present evidence for the endogenous formation of this nitrosothiol. These findings provide the first evidence for the formation of a novel nitrosothiol generated by reaction between H2S and *NO. We propose that generation of this nitrosothiol in the body may regulate the physiological effects of both *NO and H2S. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/16540095/Evidence_for_the_formation_of_a_novel_nitrosothiol_from_the_gaseous_mediators_nitric_oxide_and_hydrogen_sulphide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(06)00456-6 DB - PRIME DP - Unbound Medicine ER -