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Antioxidant enzyme inhibitors enhance nitric oxide-induced cell death in U937 cells.
Biochimie. 2006 Jul; 88(7):869-78.B

Abstract

Nitric oxide (NO), a radical species produced by many types of cells, is known to play a critical role in many regulatory processes, yet it may also participate in collateral reactions at higher concentrations, leading to cellular oxidative damage. The protective role of antioxidant enzymes against NO-induced oxidative damage in U937 cells was investigated in control and cells pre-treated with diethyldithiocarbamic acid, aminotriazole, and oxlalomalate, specific inhibitors of superoxide dismutase, catalase, and NADP(+)-dependent isocitrate dehydrogenase, respectively. Upon exposure to 1 mM S-nitroso-N-acetylpenicillamine (SNAP), the nitric oxide donor, to U937 cells, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage reflected by an increase in 8-hydroxy-2'-deoxyguanosine, were higher in inhibitor-treated cells as compared to control cells. We also observed the significant increase in the endogenous production of reactive oxygen species, as measured by the oxidation of 2'7'-dichlorodihydrofluorescin as well as the significant decrease in the intracellular GSH level in inhibitor-treated U937 cells upon exposure to NO. Upon exposure to 0.2 mM SNAP, which induced apoptotic cell death, a clear inverse relationship was observed between the control and inhibitor-treated U937 cells in their susceptibility to apoptosis. These results suggest that antioxidant enzymes play an important role in cellular defense against NO-induced cell death including necrosis and apoptosis.

Authors+Show Affiliations

Department of Biochemistry, College of Natural Sciences, Kyungpook National University, Taegu 702-701, South Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16540229

Citation

Yang, E S., and J-W Park. "Antioxidant Enzyme Inhibitors Enhance Nitric Oxide-induced Cell Death in U937 Cells." Biochimie, vol. 88, no. 7, 2006, pp. 869-78.
Yang ES, Park JW. Antioxidant enzyme inhibitors enhance nitric oxide-induced cell death in U937 cells. Biochimie. 2006;88(7):869-78.
Yang, E. S., & Park, J. W. (2006). Antioxidant enzyme inhibitors enhance nitric oxide-induced cell death in U937 cells. Biochimie, 88(7), 869-78.
Yang ES, Park JW. Antioxidant Enzyme Inhibitors Enhance Nitric Oxide-induced Cell Death in U937 Cells. Biochimie. 2006;88(7):869-78. PubMed PMID: 16540229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant enzyme inhibitors enhance nitric oxide-induced cell death in U937 cells. AU - Yang,E S, AU - Park,J-W, Y1 - 2006/02/28/ PY - 2005/06/27/received PY - 2006/02/04/accepted PY - 2006/3/17/pubmed PY - 2006/9/27/medline PY - 2006/3/17/entrez SP - 869 EP - 78 JF - Biochimie JO - Biochimie VL - 88 IS - 7 N2 - Nitric oxide (NO), a radical species produced by many types of cells, is known to play a critical role in many regulatory processes, yet it may also participate in collateral reactions at higher concentrations, leading to cellular oxidative damage. The protective role of antioxidant enzymes against NO-induced oxidative damage in U937 cells was investigated in control and cells pre-treated with diethyldithiocarbamic acid, aminotriazole, and oxlalomalate, specific inhibitors of superoxide dismutase, catalase, and NADP(+)-dependent isocitrate dehydrogenase, respectively. Upon exposure to 1 mM S-nitroso-N-acetylpenicillamine (SNAP), the nitric oxide donor, to U937 cells, the viability was lower and the protein oxidation, lipid peroxidation and oxidative DNA damage reflected by an increase in 8-hydroxy-2'-deoxyguanosine, were higher in inhibitor-treated cells as compared to control cells. We also observed the significant increase in the endogenous production of reactive oxygen species, as measured by the oxidation of 2'7'-dichlorodihydrofluorescin as well as the significant decrease in the intracellular GSH level in inhibitor-treated U937 cells upon exposure to NO. Upon exposure to 0.2 mM SNAP, which induced apoptotic cell death, a clear inverse relationship was observed between the control and inhibitor-treated U937 cells in their susceptibility to apoptosis. These results suggest that antioxidant enzymes play an important role in cellular defense against NO-induced cell death including necrosis and apoptosis. SN - 0300-9084 UR - https://www.unboundmedicine.com/medline/citation/16540229/Antioxidant_enzyme_inhibitors_enhance_nitric_oxide_induced_cell_death_in_U937_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0300-9084(06)00016-2 DB - PRIME DP - Unbound Medicine ER -