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Involvement of endogenous nitric oxide in myeloperoxidase mediated benzo(a)pyrene induced polymorphonuclear leukocytes injury.
Mol Cell Biochem. 2006 Jun; 286(1-2):43-51.MC

Abstract

The present study was undertaken to investigate the involvement of nitric oxide in the augmentation of benzo(a)pyrene induced cellular injury in polymorphonuclear leukocytes (PMNs). Polymorphs were isolated from the blood collected from Wistar rats treated with and without benzo(a)pyrene (50mg/kg, i.p.) through cardiac puncture. Catalase, superoxide dismutase (SOD), glutathione-s-transferase (GST), myeloperoxidase (MPO) and nitrite content were estimated in PMNs using standard procedures. Inducible nitric oxide synthase (iNOS) and cytochrome P-4501A1 (CYP1A1) expression in PMNs were also analyzed in presence or absence of nitric oxide synthase (NOS) inhibitors, aminoguanidine (AG, 5mM) and L-NG nitro L-arginine methyl ester (L-NAME, 1mM). A significant augmentation was observed in the nitrite content, activities of superoxide dismutase, MPO and GST and the expressions of iNOS and CYP1A1, however, catalase activity was attenuated in PMNs of benzo(a)pyrene treated rats as compared with their respective controls. AG and L-NAME resulted in a significant attenuation in nitrite content, MPO activity and iNOS expression; however, no significant alteration was observed in CYP1A1 expression. CYP1A1 inhibitor alpha-naphthoflavone inhibited the expression of iNOS in PMNs of benzo(a)pyrene treated animals significantly. The results obtained thus suggest that CYP1A1 induces iNOS expression leading to the generation of endogenous nitric oxide (NO) that could be responsible for the augmentation of myeloperoxidase-mediated benzo(a)pyrene-induced injury in PMNs.

Authors+Show Affiliations

Industrial Toxicology Research Centre (ITRC), Lucknow 226 001, UP, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16541199

Citation

Kumar, Abhai, et al. "Involvement of Endogenous Nitric Oxide in Myeloperoxidase Mediated Benzo(a)pyrene Induced Polymorphonuclear Leukocytes Injury." Molecular and Cellular Biochemistry, vol. 286, no. 1-2, 2006, pp. 43-51.
Kumar A, Patel S, Gupta YK, et al. Involvement of endogenous nitric oxide in myeloperoxidase mediated benzo(a)pyrene induced polymorphonuclear leukocytes injury. Mol Cell Biochem. 2006;286(1-2):43-51.
Kumar, A., Patel, S., Gupta, Y. K., & Singh, M. P. (2006). Involvement of endogenous nitric oxide in myeloperoxidase mediated benzo(a)pyrene induced polymorphonuclear leukocytes injury. Molecular and Cellular Biochemistry, 286(1-2), 43-51.
Kumar A, et al. Involvement of Endogenous Nitric Oxide in Myeloperoxidase Mediated Benzo(a)pyrene Induced Polymorphonuclear Leukocytes Injury. Mol Cell Biochem. 2006;286(1-2):43-51. PubMed PMID: 16541199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of endogenous nitric oxide in myeloperoxidase mediated benzo(a)pyrene induced polymorphonuclear leukocytes injury. AU - Kumar,Abhai, AU - Patel,Suman, AU - Gupta,Yogendra Kumar, AU - Singh,Mahendra Pratap, Y1 - 2006/03/16/ PY - 2005/09/28/received PY - 2005/11/21/accepted PY - 2006/3/17/pubmed PY - 2006/10/20/medline PY - 2006/3/17/entrez SP - 43 EP - 51 JF - Molecular and cellular biochemistry JO - Mol Cell Biochem VL - 286 IS - 1-2 N2 - The present study was undertaken to investigate the involvement of nitric oxide in the augmentation of benzo(a)pyrene induced cellular injury in polymorphonuclear leukocytes (PMNs). Polymorphs were isolated from the blood collected from Wistar rats treated with and without benzo(a)pyrene (50mg/kg, i.p.) through cardiac puncture. Catalase, superoxide dismutase (SOD), glutathione-s-transferase (GST), myeloperoxidase (MPO) and nitrite content were estimated in PMNs using standard procedures. Inducible nitric oxide synthase (iNOS) and cytochrome P-4501A1 (CYP1A1) expression in PMNs were also analyzed in presence or absence of nitric oxide synthase (NOS) inhibitors, aminoguanidine (AG, 5mM) and L-NG nitro L-arginine methyl ester (L-NAME, 1mM). A significant augmentation was observed in the nitrite content, activities of superoxide dismutase, MPO and GST and the expressions of iNOS and CYP1A1, however, catalase activity was attenuated in PMNs of benzo(a)pyrene treated rats as compared with their respective controls. AG and L-NAME resulted in a significant attenuation in nitrite content, MPO activity and iNOS expression; however, no significant alteration was observed in CYP1A1 expression. CYP1A1 inhibitor alpha-naphthoflavone inhibited the expression of iNOS in PMNs of benzo(a)pyrene treated animals significantly. The results obtained thus suggest that CYP1A1 induces iNOS expression leading to the generation of endogenous nitric oxide (NO) that could be responsible for the augmentation of myeloperoxidase-mediated benzo(a)pyrene-induced injury in PMNs. SN - 0300-8177 UR - https://www.unboundmedicine.com/medline/citation/16541199/Involvement_of_endogenous_nitric_oxide_in_myeloperoxidase_mediated_benzo_a_pyrene_induced_polymorphonuclear_leukocytes_injury_ L2 - https://doi.org/10.1007/s11010-005-9083-5 DB - PRIME DP - Unbound Medicine ER -