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Genetic associations between cathepsin D exon 2 C-->T polymorphism and Alzheimer's disease, and pathological correlations with genotype.
J Neurol Neurosurg Psychiatry 2006; 77(4):515-7JN

Abstract

Genetic variations represent major risk factors for Alzheimer's disease (AD). While familial early onset AD is associated with mutations in the amyloid precursor protein and presenilin genes, only the e4 allele of the apolipoprotein E (APOE) gene has so far been established as a genetic risk factor for late onset familial and sporadic AD. It has been suggested that the C-->T (224Ala-->Val) transition within exon 2 of the cathepsin D gene (CTSD) might represent a risk factor for late onset AD. The objective of this study was to investigate whether possession of the CTSD exon 2 T allele increases the risk of developing AD, and to determine whether this modulates the amyloid pathology of the disease in conjunction with, or independent of, the APOE e4 allele. Blood samples were obtained from 412 patients with possible or probable AD and brain tissues from a further 148 patients with AD confirmed by postmortem examination. CTSD and APOE genotyping were performed by PCR on DNA extracted from blood, or from frontal cortex or cerebellum in the postmortem cases. Pathological measures of amyloid beta protein (Abeta), as plaque Abeta40 and Abeta42(3) load and degree of cerebral amyloid angiopathy were made by image analysis or semiquantitative rating, respectively. CTSD genotype frequencies in AD were not significantly different from those in control subjects, nor did these differ between cases of early or late onset AD or between younger and older controls. There was no gene interaction between the CTSD T and APOE e4 alleles. The amount of plaque Abeta40 was greater in patients carrying the CTSD T allele than in non-carriers, and in patients bearing APOE e4 allele compared with non-carriers. Possession of both these alleles acted synergistically to increase levels of plaque Abeta40, especially in those individuals who were homozygous for the APOE e4 allele. Possession of the CTSD T allele had no effect on plaque Abeta42(3) load or degree of CAA. Possession of the CTSD T allele does not increase the risk of developing AD per se, but has a modulating effect on the pathogenesis of the disorder by increasing, in concert with the APOE e4 allele, the amount of Abeta deposited as senile plaques in the brain in the form of Abeta40.

Authors+Show Affiliations

Clinical Neuroscience Research Group, University of Manchester, Greater Manchester Neurosciences Centre, Hope Hospital, Stott Lane, Salford M6 8HD, Manchester, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16543533

Citation

Davidson, Y, et al. "Genetic Associations Between Cathepsin D Exon 2 C-->T Polymorphism and Alzheimer's Disease, and Pathological Correlations With Genotype." Journal of Neurology, Neurosurgery, and Psychiatry, vol. 77, no. 4, 2006, pp. 515-7.
Davidson Y, Gibbons L, Pritchard A, et al. Genetic associations between cathepsin D exon 2 C-->T polymorphism and Alzheimer's disease, and pathological correlations with genotype. J Neurol Neurosurg Psychiatry. 2006;77(4):515-7.
Davidson, Y., Gibbons, L., Pritchard, A., Hardicre, J., Wren, J., Tian, J., ... Mann, D. M. (2006). Genetic associations between cathepsin D exon 2 C-->T polymorphism and Alzheimer's disease, and pathological correlations with genotype. Journal of Neurology, Neurosurgery, and Psychiatry, 77(4), pp. 515-7.
Davidson Y, et al. Genetic Associations Between Cathepsin D Exon 2 C-->T Polymorphism and Alzheimer's Disease, and Pathological Correlations With Genotype. J Neurol Neurosurg Psychiatry. 2006;77(4):515-7. PubMed PMID: 16543533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic associations between cathepsin D exon 2 C-->T polymorphism and Alzheimer's disease, and pathological correlations with genotype. AU - Davidson,Y, AU - Gibbons,L, AU - Pritchard,A, AU - Hardicre,J, AU - Wren,J, AU - Tian,J, AU - Shi,J, AU - Stopford,C, AU - Julien,C, AU - Thompson,J, AU - Payton,A, AU - Thaker,U, AU - Hayes,A J, AU - Iwatsubo,T, AU - Pickering-Brown,S M, AU - Pendleton,N, AU - Horan,M A, AU - Burns,A, AU - Purandare,N, AU - Lendon,C L, AU - Neary,D, AU - Snowden,J S, AU - Mann,D M A, PY - 2006/3/18/pubmed PY - 2006/5/19/medline PY - 2006/3/18/entrez SP - 515 EP - 7 JF - Journal of neurology, neurosurgery, and psychiatry JO - J. Neurol. Neurosurg. Psychiatry VL - 77 IS - 4 N2 - Genetic variations represent major risk factors for Alzheimer's disease (AD). While familial early onset AD is associated with mutations in the amyloid precursor protein and presenilin genes, only the e4 allele of the apolipoprotein E (APOE) gene has so far been established as a genetic risk factor for late onset familial and sporadic AD. It has been suggested that the C-->T (224Ala-->Val) transition within exon 2 of the cathepsin D gene (CTSD) might represent a risk factor for late onset AD. The objective of this study was to investigate whether possession of the CTSD exon 2 T allele increases the risk of developing AD, and to determine whether this modulates the amyloid pathology of the disease in conjunction with, or independent of, the APOE e4 allele. Blood samples were obtained from 412 patients with possible or probable AD and brain tissues from a further 148 patients with AD confirmed by postmortem examination. CTSD and APOE genotyping were performed by PCR on DNA extracted from blood, or from frontal cortex or cerebellum in the postmortem cases. Pathological measures of amyloid beta protein (Abeta), as plaque Abeta40 and Abeta42(3) load and degree of cerebral amyloid angiopathy were made by image analysis or semiquantitative rating, respectively. CTSD genotype frequencies in AD were not significantly different from those in control subjects, nor did these differ between cases of early or late onset AD or between younger and older controls. There was no gene interaction between the CTSD T and APOE e4 alleles. The amount of plaque Abeta40 was greater in patients carrying the CTSD T allele than in non-carriers, and in patients bearing APOE e4 allele compared with non-carriers. Possession of both these alleles acted synergistically to increase levels of plaque Abeta40, especially in those individuals who were homozygous for the APOE e4 allele. Possession of the CTSD T allele had no effect on plaque Abeta42(3) load or degree of CAA. Possession of the CTSD T allele does not increase the risk of developing AD per se, but has a modulating effect on the pathogenesis of the disorder by increasing, in concert with the APOE e4 allele, the amount of Abeta deposited as senile plaques in the brain in the form of Abeta40. SN - 0022-3050 UR - https://www.unboundmedicine.com/medline/citation/16543533/Genetic_associations_between_cathepsin_D_exon_2_C__>T_polymorphism_and_Alzheimer's_disease_and_pathological_correlations_with_genotype_ L2 - http://jnnp.bmj.com/cgi/pmidlookup?view=long&pmid=16543533 DB - PRIME DP - Unbound Medicine ER -