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Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films.
J Biomed Mater Res B Appl Biomater. 2006 Aug; 78(2):222-9.JB

Abstract

This article reports results of endothelial cell interaction with atom beam source N-doped a-C:H (diamond-like carbon, DLC) as it compares with that of Si-doped DLC thin films. The RF plasma source exhibits up to 40% N-dissociation and N-atomic fluxes of approximately 0.85 x 10(18) atoms/s, which ensures better atomic nitrogen incorporation. Two different types of nitrogen species (with and without the use of sweep plates to remove charged ions) were employed for nitrogen doping. The number of attached endothelial cells is highest on Si-DLC, followed by the N-DLC (where the sweep plates were used to remove ions), the N-DLC (without the use of sweep plates), undoped DLC, and finally the uncoated sample. The contact angle values for these films suggest that water contact angle is higher in the atomic nitrogen neutral films and Si-DLC films compared to the ionized-nitrogen specie doped films and undoped DLC thin films, suggesting that the more hydrophobic films, semiconducting films, and film with relieved stress have better interaction with human microvascular endothelial cells. It seems evident that N-doping increases the Raman I(D)/I(G) ratios, whereas N-neutral doping decreases it slightly and Si-doping decreases it even further. In this study, lower Raman I(D)/I(G) ratios are associated with increased sp(3)/sp(2) ratio, an increased H concentration, photoluminescence intensity, and a higher endothelial cellular adhesion. These investigations could be relevant to biocompatibility assessment of nanostructured biomaterials and tissue engineering.

Authors+Show Affiliations

NIBEC, School of Electrical and Mechanical Engineering, University of Ulster, United Kingdom. thoms@nibec-s1.nibec.ulst.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16544310

Citation

Okpalugo, T I T., et al. "Human Microvascular Endothelial Cellular Interaction With Atomic N-doped DLC Compared With Si-doped DLC Thin Films." Journal of Biomedical Materials Research. Part B, Applied Biomaterials, vol. 78, no. 2, 2006, pp. 222-9.
Okpalugo TI, Murphy H, Ogwu AA, et al. Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films. J Biomed Mater Res Part B Appl Biomater. 2006;78(2):222-9.
Okpalugo, T. I., Murphy, H., Ogwu, A. A., Abbas, G., Ray, S. C., Maguire, P. D., McLaughlin, J., & McCullough, R. W. (2006). Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 78(2), 222-9.
Okpalugo TI, et al. Human Microvascular Endothelial Cellular Interaction With Atomic N-doped DLC Compared With Si-doped DLC Thin Films. J Biomed Mater Res Part B Appl Biomater. 2006;78(2):222-9. PubMed PMID: 16544310.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human microvascular endothelial cellular interaction with atomic N-doped DLC compared with Si-doped DLC thin films. AU - Okpalugo,T I T, AU - Murphy,H, AU - Ogwu,A A, AU - Abbas,G, AU - Ray,S C, AU - Maguire,P D, AU - McLaughlin,J, AU - McCullough,R W, PY - 2006/3/18/pubmed PY - 2006/9/15/medline PY - 2006/3/18/entrez SP - 222 EP - 9 JF - Journal of biomedical materials research. Part B, Applied biomaterials JO - J. Biomed. Mater. Res. Part B Appl. Biomater. VL - 78 IS - 2 N2 - This article reports results of endothelial cell interaction with atom beam source N-doped a-C:H (diamond-like carbon, DLC) as it compares with that of Si-doped DLC thin films. The RF plasma source exhibits up to 40% N-dissociation and N-atomic fluxes of approximately 0.85 x 10(18) atoms/s, which ensures better atomic nitrogen incorporation. Two different types of nitrogen species (with and without the use of sweep plates to remove charged ions) were employed for nitrogen doping. The number of attached endothelial cells is highest on Si-DLC, followed by the N-DLC (where the sweep plates were used to remove ions), the N-DLC (without the use of sweep plates), undoped DLC, and finally the uncoated sample. The contact angle values for these films suggest that water contact angle is higher in the atomic nitrogen neutral films and Si-DLC films compared to the ionized-nitrogen specie doped films and undoped DLC thin films, suggesting that the more hydrophobic films, semiconducting films, and film with relieved stress have better interaction with human microvascular endothelial cells. It seems evident that N-doping increases the Raman I(D)/I(G) ratios, whereas N-neutral doping decreases it slightly and Si-doping decreases it even further. In this study, lower Raman I(D)/I(G) ratios are associated with increased sp(3)/sp(2) ratio, an increased H concentration, photoluminescence intensity, and a higher endothelial cellular adhesion. These investigations could be relevant to biocompatibility assessment of nanostructured biomaterials and tissue engineering. SN - 1552-4973 UR - https://www.unboundmedicine.com/medline/citation/16544310/Human_microvascular_endothelial_cellular_interaction_with_atomic_N_doped_DLC_compared_with_Si_doped_DLC_thin_films_ L2 - https://doi.org/10.1002/jbm.b.30459 DB - PRIME DP - Unbound Medicine ER -