[A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system].
Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Dec; 25(12):1995-7.GP

Abstract

A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10(-8) mol x L(-1) for FITC-labeled phenylalanine at a signal-to-noise ratio (S/N) of 3 (on-column, 0.05 mm i.d.). The system exhibited a good linear response in the range of 1 x 10(-7) -2 x 10(-5) mol x L(-1) (r2 = 0.999). The relative standard deviation (RSD) of 6 repetitive injections on peak heights is less than 3%.

Authors+Show Affiliations

Yang BC

Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.

Tan F

No affiliation info available

Guan YF

No affiliation info available

Pub Type(s)

English Abstract

Journal Article

Language

chi

PubMed ID

16544490

Citation

Yang, Bing-Cheng, et al. "[A Novel Light-emitting Diode-induced Fluorescence Detector for Micro-scale Flow Analysis System]." Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu, vol. 25, no. 12, 2005, pp. 1995-7.

Yang BC, Tan F, Guan YF. [A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system]. Guang Pu Xue Yu Guang Pu Fen Xi. 2005;25(12):1995-7.

Yang, B. C., Tan, F., & Guan, Y. F. (2005). [A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system]. Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu, 25(12), 1995-7.

Yang BC, Tan F, Guan YF. [A Novel Light-emitting Diode-induced Fluorescence Detector for Micro-scale Flow Analysis System]. Guang Pu Xue Yu Guang Pu Fen Xi. 2005;25(12):1995-7. PubMed PMID: 16544490.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - [A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system]. AU - Yang,Bing-Cheng, AU - Tan,Feng, AU - Guan,Ya-Feng, PY - 2006/3/21/pubmed PY - 2006/3/21/medline PY - 2006/3/21/entrez SP - 1995 EP - 7 JF - Guang pu xue yu guang pu fen xi = Guang pu JO - Guang Pu Xue Yu Guang Pu Fen Xi VL - 25 IS - 12 N2 - A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10(-8) mol x L(-1) for FITC-labeled phenylalanine at a signal-to-noise ratio (S/N) of 3 (on-column, 0.05 mm i.d.). The system exhibited a good linear response in the range of 1 x 10(-7) -2 x 10(-5) mol x L(-1) (r2 = 0.999). The relative standard deviation (RSD) of 6 repetitive injections on peak heights is less than 3%. SN - 1000-0593 UR - https://www.unboundmedicine.com/medline/citation/16544490/[A_novel_light_emitting_diode_induced_fluorescence_detector_for_micro_scale_flow_analysis_system]_ DB - PRIME DP - Unbound Medicine ER -

Tags

[A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system].Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Dec; 25(12):1995-7.GP