[A novel light-emitting diode-induced fluorescence detector for micro-scale flow analysis system].Guang Pu Xue Yu Guang Pu Fen Xi. 2005 Dec; 25(12):1995-7.GP
A compact light-emitting diode-induced fluorescence detector based on collinear scheme is described. The detector was assembled by all-solid-state optical-electronic components and easily coupled with micro-scale flow analysis system by on-column detection mode. Fluorescein isothiocyanate (FITC) and FITC-labeled amino acids were used as test analytes to evaluate the detector performance by capillary electrophoresis and flow injection analysis techniques. The concentration limit of detection was 10(-8) mol x L(-1) for FITC-labeled phenylalanine at a signal-to-noise ratio (S/N) of 3 (on-column, 0.05 mm i.d.). The system exhibited a good linear response in the range of 1 x 10(-7) -2 x 10(-5) mol x L(-1) (r2 = 0.999). The relative standard deviation (RSD) of 6 repetitive injections on peak heights is less than 3%.