Complexation and time-dependent accumulation of copper by larval fathead minnows (Pimephales promelas): implications for modeling toxicity.
Aquat Toxicol. 2006 Jun 10; 78(1):42-9.AT

Abstract

Mechanistic models predicting copper (Cu) toxicity to aquatic biota in natural waters require organic and inorganic water chemistry, and quantified values for Cu binding by sensitive biological receptors. In bioaccumulation experiments using larval fathead minnows (FHM; Pimephales promelas), we investigated time to asymptotic accumulation of Cu and quantified the conditional stability constants (binding affinity; log K(Cu-FHM)) and binding-site densities of Cu-FHM complexation. Cu bioaccumulation increased rapidly, approaching an asymptote in exposures longer than 12 h, indicating that Cu loading at 24 h is an appropriate exposure duration for modeling Cu complexation by larval FHM. Results of Langmuir and Scatchard analyses of other bioaccumulation experiments produced log K(Cu-FHM) values of 6.52, and binding-site densities of 0.39 micromol g(-1)dry weight. These whole-body log K(Cu-FHM) values are approximately an order of magnitude lower than those reported for adult FHM gills. However, binding-site densities for larval and adult FHM are similar. Under similar test conditions, comparable concentrations of aqueous Cu cause 50% mortality in adult and larval FHM suggesting that binding site densities determine comparable metal-tissue loadings and have greater influence on Cu bioavailability than binding affinity.

Authors+Show Affiliations

Brooks ML

Department of Zoology and Physiology, University of Wyoming, Laramie, WY 82071, USA. brooks@nceas.ucsb.edu

Boese CJ

No affiliation info available

Meyer JS

No affiliation info available

MeSH

AnimalsBinding SitesCopperCyprinidaeLarvaTime FactorsWaterWater Pollutants, Chemical

Pub Type(s)

Comparative Study

Journal Article

Research Support, Non-U.S. Gov't

Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

16546273

Citation

Brooks, Marjorie L., et al. "Complexation and Time-dependent Accumulation of Copper By Larval Fathead Minnows (Pimephales Promelas): Implications for Modeling Toxicity." Aquatic Toxicology (Amsterdam, Netherlands), vol. 78, no. 1, 2006, pp. 42-9.

Brooks ML, Boese CJ, Meyer JS. Complexation and time-dependent accumulation of copper by larval fathead minnows (Pimephales promelas): implications for modeling toxicity. Aquat Toxicol. 2006;78(1):42-9.

Brooks, M. L., Boese, C. J., & Meyer, J. S. (2006). Complexation and time-dependent accumulation of copper by larval fathead minnows (Pimephales promelas): implications for modeling toxicity. Aquatic Toxicology (Amsterdam, Netherlands), 78(1), 42-9.

Brooks ML, Boese CJ, Meyer JS. Complexation and Time-dependent Accumulation of Copper By Larval Fathead Minnows (Pimephales Promelas): Implications for Modeling Toxicity. Aquat Toxicol. 2006 Jun 10;78(1):42-9. PubMed PMID: 16546273.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Complexation and time-dependent accumulation of copper by larval fathead minnows (Pimephales promelas): implications for modeling toxicity. AU - Brooks,Marjorie L, AU - Boese,Connie J, AU - Meyer,Joseph S, Y1 - 2006/03/20/ PY - 2005/04/12/received PY - 2006/02/01/revised PY - 2006/02/08/accepted PY - 2006/3/21/pubmed PY - 2007/10/25/medline PY - 2006/3/21/entrez SP - 42 EP - 9 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 78 IS - 1 N2 - Mechanistic models predicting copper (Cu) toxicity to aquatic biota in natural waters require organic and inorganic water chemistry, and quantified values for Cu binding by sensitive biological receptors. In bioaccumulation experiments using larval fathead minnows (FHM; Pimephales promelas), we investigated time to asymptotic accumulation of Cu and quantified the conditional stability constants (binding affinity; log K(Cu-FHM)) and binding-site densities of Cu-FHM complexation. Cu bioaccumulation increased rapidly, approaching an asymptote in exposures longer than 12 h, indicating that Cu loading at 24 h is an appropriate exposure duration for modeling Cu complexation by larval FHM. Results of Langmuir and Scatchard analyses of other bioaccumulation experiments produced log K(Cu-FHM) values of 6.52, and binding-site densities of 0.39 micromol g(-1)dry weight. These whole-body log K(Cu-FHM) values are approximately an order of magnitude lower than those reported for adult FHM gills. However, binding-site densities for larval and adult FHM are similar. Under similar test conditions, comparable concentrations of aqueous Cu cause 50% mortality in adult and larval FHM suggesting that binding site densities determine comparable metal-tissue loadings and have greater influence on Cu bioavailability than binding affinity. SN - 0166-445X UR - https://www.unboundmedicine.com/medline/citation/16546273/Complexation_and_time_dependent_accumulation_of_copper_by_larval_fathead_minnows__Pimephales_promelas_:_implications_for_modeling_toxicity_ DB - PRIME DP - Unbound Medicine ER -

Tags

Complexation and time-dependent accumulation of copper by larval fathead minnows (Pimephales promelas): implications for modeling toxicity.Aquat Toxicol. 2006 Jun 10; 78(1):42-9.AT