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Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae.
Diagn Microbiol Infect Dis. 2006 May; 55(1):37-45.DM

Abstract

Burkholderia mallei and Burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category B biothreat agents. We used an in silico approach to compare the B. mallei ATCC 23344 and B. pseudomallei K96243 genomes to identify nucleotide sequences unique to B. mallei. Five distinct B. mallei DNA sequences and/or genes were identified and evaluated for polymerase chain reaction (PCR) assay development. Genomic DNAs from a collection of 31 B. mallei and 34 B. pseudomallei isolates, obtained from various geographic, clinical, and environmental sources over a 70-year period, were tested with PCR primers targeted for each of the B. mallei ATCC 23344-specific nucleotide sequences. Of the 5 chromosomal targets analyzed, only PCR primers designed to bimA(Bm) were specific for B. mallei. These primers were used to develop a rapid PCR assay for the definitive identification of B. mallei and differentiation from all other bacteria.

Authors+Show Affiliations

Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16546342

Citation

Ulrich, Ricky L., et al. "Development of a Polymerase Chain Reaction Assay for the Specific Identification of Burkholderia Mallei and Differentiation From Burkholderia Pseudomallei and Other Closely Related Burkholderiaceae." Diagnostic Microbiology and Infectious Disease, vol. 55, no. 1, 2006, pp. 37-45.
Ulrich RL, Ulrich MP, Schell MA, et al. Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae. Diagn Microbiol Infect Dis. 2006;55(1):37-45.
Ulrich, R. L., Ulrich, M. P., Schell, M. A., Kim, H. S., & DeShazer, D. (2006). Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae. Diagnostic Microbiology and Infectious Disease, 55(1), 37-45.
Ulrich RL, et al. Development of a Polymerase Chain Reaction Assay for the Specific Identification of Burkholderia Mallei and Differentiation From Burkholderia Pseudomallei and Other Closely Related Burkholderiaceae. Diagn Microbiol Infect Dis. 2006;55(1):37-45. PubMed PMID: 16546342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a polymerase chain reaction assay for the specific identification of Burkholderia mallei and differentiation from Burkholderia pseudomallei and other closely related Burkholderiaceae. AU - Ulrich,Ricky L, AU - Ulrich,Melanie P, AU - Schell,Mark A, AU - Kim,H Stanley, AU - DeShazer,David, Y1 - 2006/03/20/ PY - 2005/08/25/received PY - 2005/11/14/revised PY - 2005/11/29/accepted PY - 2006/3/21/pubmed PY - 2006/8/19/medline PY - 2006/3/21/entrez SP - 37 EP - 45 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 55 IS - 1 N2 - Burkholderia mallei and Burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category B biothreat agents. We used an in silico approach to compare the B. mallei ATCC 23344 and B. pseudomallei K96243 genomes to identify nucleotide sequences unique to B. mallei. Five distinct B. mallei DNA sequences and/or genes were identified and evaluated for polymerase chain reaction (PCR) assay development. Genomic DNAs from a collection of 31 B. mallei and 34 B. pseudomallei isolates, obtained from various geographic, clinical, and environmental sources over a 70-year period, were tested with PCR primers targeted for each of the B. mallei ATCC 23344-specific nucleotide sequences. Of the 5 chromosomal targets analyzed, only PCR primers designed to bimA(Bm) were specific for B. mallei. These primers were used to develop a rapid PCR assay for the definitive identification of B. mallei and differentiation from all other bacteria. SN - 0732-8893 UR - https://www.unboundmedicine.com/medline/citation/16546342/Development_of_a_polymerase_chain_reaction_assay_for_the_specific_identification_of_Burkholderia_mallei_and_differentiation_from_Burkholderia_pseudomallei_and_other_closely_related_Burkholderiaceae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(05)00350-0 DB - PRIME DP - Unbound Medicine ER -