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[Screening and identification of therapeutic effect evaluation antigens of angiostrongyliasis].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Mar; 26(3):284-6, 289.NF

Abstract

OBJECTIVE

To identify antigens which may help evaluate the therapeutic effect of angiostrongyliasis from adult worm antigen of Angiostrongylus cantonensis.

METHODS

The adult worm antigens of A. cantonensis were analyzed by Western blotting with the sera of rats infected with A. cantonensis before and after treatment. The sera of rats were tested by enzyme-linked immunosorbent assay (ELISA).

RESULTS

The antigens with relative molecular mass between 38,000 and 78,000 reacted not only with the sera of rats before treatment, but also with that after treatment. The antigens with M(r) between 190,000 and 17,000 reacted with the sera of rats before treatment but not with that after treatment; those with M(r) between 32,000 and 24,000 antigens strongly reacted with the former, but the reaction became much weakened with the latter. The AC32-IgG antibody appeared earlier than the AC-IgG, and disappeared rapidly after treatment. Six of the 10 treated rats became negative for AC-IgG as found by ELISA.

CONCLUSION

The antigens of adult worm antigen of A. cantonensis with M(r) of 190,000, 32,000, 24,000, 17,000 and 16,000 may serve as candidate antigens for therapeutic effect evaluation of angiostrongyliasis.

Authors+Show Affiliations

Department of Pathogenic Biology, Southern Medical University, Guangzhou 510515, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16546727

Citation

Zhao, Xing-cun, et al. "[Screening and Identification of Therapeutic Effect Evaluation Antigens of Angiostrongyliasis]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 3, 2006, pp. 284-6, 289.
Zhao XC, Gu JB, Li H, et al. [Screening and identification of therapeutic effect evaluation antigens of angiostrongyliasis]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(3):284-6, 289.
Zhao, X. C., Gu, J. B., Li, H., Liu, M., Shen, H. X., & Chen, X. G. (2006). [Screening and identification of therapeutic effect evaluation antigens of angiostrongyliasis]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(3), 284-6, 289.
Zhao XC, et al. [Screening and Identification of Therapeutic Effect Evaluation Antigens of Angiostrongyliasis]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(3):284-6, 289. PubMed PMID: 16546727.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Screening and identification of therapeutic effect evaluation antigens of angiostrongyliasis]. AU - Zhao,Xing-cun, AU - Gu,Jin-bao, AU - Li,Hua, AU - Liu,Min, AU - Shen,Hao-xian, AU - Chen,Xiao-guang, PY - 2006/3/21/pubmed PY - 2008/4/15/medline PY - 2006/3/21/entrez SP - 284-6, 289 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 3 N2 - OBJECTIVE: To identify antigens which may help evaluate the therapeutic effect of angiostrongyliasis from adult worm antigen of Angiostrongylus cantonensis. METHODS: The adult worm antigens of A. cantonensis were analyzed by Western blotting with the sera of rats infected with A. cantonensis before and after treatment. The sera of rats were tested by enzyme-linked immunosorbent assay (ELISA). RESULTS: The antigens with relative molecular mass between 38,000 and 78,000 reacted not only with the sera of rats before treatment, but also with that after treatment. The antigens with M(r) between 190,000 and 17,000 reacted with the sera of rats before treatment but not with that after treatment; those with M(r) between 32,000 and 24,000 antigens strongly reacted with the former, but the reaction became much weakened with the latter. The AC32-IgG antibody appeared earlier than the AC-IgG, and disappeared rapidly after treatment. Six of the 10 treated rats became negative for AC-IgG as found by ELISA. CONCLUSION: The antigens of adult worm antigen of A. cantonensis with M(r) of 190,000, 32,000, 24,000, 17,000 and 16,000 may serve as candidate antigens for therapeutic effect evaluation of angiostrongyliasis. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/16546727/[Screening_and_identification_of_therapeutic_effect_evaluation_antigens_of_angiostrongyliasis]_ DB - PRIME DP - Unbound Medicine ER -