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[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Mar; 26(3):290-3.NF

Abstract

OBJECTIVE

To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins.

METHODS

BALB/c mice were immunized with the recombinant N proteins of SARS-CoV, 229E and OC43 to obtain the mAbs by means of hybridoma. Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay. Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs.

RESULTS

The mAbs against the recombinant N proteins of SARS-CoV, 229E and OC43 were obtained, which reacted specifically with the corresponding viral N protein as shown by indirect ELISA, Western blotting and indirect immunofluorescence assay. No cross-reactivity was found between the 3 N proteins.

CONCLUSION

The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses.

Authors+Show Affiliations

Qiu LW
Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Wang YD
No affiliation info available
Liao ZY
No affiliation info available
Wen K
No affiliation info available
Che XY
No affiliation info available

MeSH

AnimalsAntibodies, MonoclonalBlotting, WesternCoronavirus 229E, HumanCoronavirus OC43, HumanCross ReactionsEnzyme-Linked Immunosorbent AssayFemaleFluorescent Antibody Technique, IndirectHumansMiceMice, Inbred BALB CNucleocapsid ProteinsRecombinant ProteinsSARS Virus

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16546729

Citation

Qiu, Li-wen, et al. "[Antigenicity Analysis of Nucleocapsid Proteins of 3 Human Coronaviruses SARS-CoV, 229E and OC43 With Their Monoclonal Antibodies]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 3, 2006, pp. 290-3.
Qiu LW, Wang YD, Liao ZY, et al. [Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(3):290-3.
Qiu, L. W., Wang, Y. D., Liao, Z. Y., Wen, K., & Che, X. Y. (2006). [Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(3), 290-3.
Qiu LW, et al. [Antigenicity Analysis of Nucleocapsid Proteins of 3 Human Coronaviruses SARS-CoV, 229E and OC43 With Their Monoclonal Antibodies]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(3):290-3. PubMed PMID: 16546729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies]. AU - Qiu,Li-wen, AU - Wang,Ya-di, AU - Liao,Zhi-yong, AU - Wen,Kun, AU - Che,Xiao-yan, PY - 2006/3/21/pubmed PY - 2008/4/15/medline PY - 2006/3/21/entrez SP - 290 EP - 3 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 3 N2 - OBJECTIVE: To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins. METHODS: BALB/c mice were immunized with the recombinant N proteins of SARS-CoV, 229E and OC43 to obtain the mAbs by means of hybridoma. Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay. Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs. RESULTS: The mAbs against the recombinant N proteins of SARS-CoV, 229E and OC43 were obtained, which reacted specifically with the corresponding viral N protein as shown by indirect ELISA, Western blotting and indirect immunofluorescence assay. No cross-reactivity was found between the 3 N proteins. CONCLUSION: The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/16546729/[Antigenicity_analysis_of_nucleocapsid_proteins_of_3_human_coronaviruses_SARS_CoV_229E_and_OC43_with_their_monoclonal_antibodies]_ L2 - http://www.diseaseinfosearch.org/result/6415 DB - PRIME DP - Unbound Medicine ER -

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