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[Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies].
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Mar; 26(3):290-3.NF

Abstract

OBJECTIVE

To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins.

METHODS

BALB/c mice were immunized with the recombinant N proteins of SARS-CoV, 229E and OC43 to obtain the mAbs by means of hybridoma. Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay. Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs.

RESULTS

The mAbs against the recombinant N proteins of SARS-CoV, 229E and OC43 were obtained, which reacted specifically with the corresponding viral N protein as shown by indirect ELISA, Western blotting and indirect immunofluorescence assay. No cross-reactivity was found between the 3 N proteins.

CONCLUSION

The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses.

Authors+Show Affiliations

Central Laboratory, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16546729

Citation

Qiu, Li-wen, et al. "[Antigenicity Analysis of Nucleocapsid Proteins of 3 Human Coronaviruses SARS-CoV, 229E and OC43 With Their Monoclonal Antibodies]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 26, no. 3, 2006, pp. 290-3.
Qiu LW, Wang YD, Liao ZY, et al. [Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(3):290-3.
Qiu, L. W., Wang, Y. D., Liao, Z. Y., Wen, K., & Che, X. Y. (2006). [Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 26(3), 290-3.
Qiu LW, et al. [Antigenicity Analysis of Nucleocapsid Proteins of 3 Human Coronaviruses SARS-CoV, 229E and OC43 With Their Monoclonal Antibodies]. Nan Fang Yi Ke Da Xue Xue Bao. 2006;26(3):290-3. PubMed PMID: 16546729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV, 229E and OC43 with their monoclonal antibodies]. AU - Qiu,Li-wen, AU - Wang,Ya-di, AU - Liao,Zhi-yong, AU - Wen,Kun, AU - Che,Xiao-yan, PY - 2006/3/21/pubmed PY - 2008/4/15/medline PY - 2006/3/21/entrez SP - 290 EP - 3 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 26 IS - 3 N2 - OBJECTIVE: To prepare and characterize monoclonal antibodies (mAbs) against the recombinant nucleocapsid (N) protein of 3 human coronaviruses SARS-CoV, 229E and OC43 and study the antigenic relationship between the 3 N proteins. METHODS: BALB/c mice were immunized with the recombinant N proteins of SARS-CoV, 229E and OC43 to obtain the mAbs by means of hybridoma. Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay (ELISA), Western blotting and indirect immunofluorescence assay. Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs. RESULTS: The mAbs against the recombinant N proteins of SARS-CoV, 229E and OC43 were obtained, which reacted specifically with the corresponding viral N protein as shown by indirect ELISA, Western blotting and indirect immunofluorescence assay. No cross-reactivity was found between the 3 N proteins. CONCLUSION: The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/16546729/[Antigenicity_analysis_of_nucleocapsid_proteins_of_3_human_coronaviruses_SARS_CoV_229E_and_OC43_with_their_monoclonal_antibodies]_ L2 - http://www.diseaseinfosearch.org/result/6415 DB - PRIME DP - Unbound Medicine ER -