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Modulation of oxidative cell damage by reconstituted mixtures of phenolic apple juice extracts in human colon cell lines.
Mol Nutr Food Res. 2006 Apr; 50(4-5):413-7.MN

Abstract

Diets rich in fruits and vegetables are associated with a lower risk of tumour induction in the intestine and other sites. Apple juice with high amounts of antioxidative phenolics might protect the intestine against reactive oxygen species-mediated cell damage. We investigated to which extent the preventive effectiveness of polyphenolic juice extracts is governed by the amounts of five major constituents (rutin, phloridzin, chlorogenic acid, caffeic acid and epicatechin). In human colon cell lines (Caco-2, HT29), reconstituted mixtures of these phenolics were investigated in comparison to the original juice extracts, originating from cider and table apples. Parameters studied were (oxidative) DNA damage (Comet assay), cellular redox status (dichlorofluorescein assay) and Trolox equivalent antioxidant capacity (TEAC). The TEAC of the reconstituted mixtures was higher compared to the respective original extracts (4.7-7.3 mM vs. 3.6-4.2 mM Trolox). After 24 h cell incubation, menadione-induced (oxidative) DNA damage was more effectively reduced by the reconstituted mixtures (1-100 microg/mL, 24 h), as compared to the original extracts. In contrast, the cellular ROS level was reduced to a rather similar extent by original extracts and reconstituted mixtures. The results lead to the conclusion that the selected constituents in their authentic proportions substantially account for the antioxidative effectiveness of phenolic apple juice extracts.

Authors+Show Affiliations

Division of Food Chemistry & Environmental Toxicology, Department of Chemistry, University of Kaiserslautern, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16548011

Citation

Schaefer, Sandra, et al. "Modulation of Oxidative Cell Damage By Reconstituted Mixtures of Phenolic Apple Juice Extracts in Human Colon Cell Lines." Molecular Nutrition & Food Research, vol. 50, no. 4-5, 2006, pp. 413-7.
Schaefer S, Baum M, Eisenbrand G, et al. Modulation of oxidative cell damage by reconstituted mixtures of phenolic apple juice extracts in human colon cell lines. Mol Nutr Food Res. 2006;50(4-5):413-7.
Schaefer, S., Baum, M., Eisenbrand, G., & Janzowski, C. (2006). Modulation of oxidative cell damage by reconstituted mixtures of phenolic apple juice extracts in human colon cell lines. Molecular Nutrition & Food Research, 50(4-5), 413-7.
Schaefer S, et al. Modulation of Oxidative Cell Damage By Reconstituted Mixtures of Phenolic Apple Juice Extracts in Human Colon Cell Lines. Mol Nutr Food Res. 2006;50(4-5):413-7. PubMed PMID: 16548011.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Modulation of oxidative cell damage by reconstituted mixtures of phenolic apple juice extracts in human colon cell lines. AU - Schaefer,Sandra, AU - Baum,Matthias, AU - Eisenbrand,Gerhard, AU - Janzowski,Christine, PY - 2006/3/21/pubmed PY - 2006/6/20/medline PY - 2006/3/21/entrez SP - 413 EP - 7 JF - Molecular nutrition & food research JO - Mol Nutr Food Res VL - 50 IS - 4-5 N2 - Diets rich in fruits and vegetables are associated with a lower risk of tumour induction in the intestine and other sites. Apple juice with high amounts of antioxidative phenolics might protect the intestine against reactive oxygen species-mediated cell damage. We investigated to which extent the preventive effectiveness of polyphenolic juice extracts is governed by the amounts of five major constituents (rutin, phloridzin, chlorogenic acid, caffeic acid and epicatechin). In human colon cell lines (Caco-2, HT29), reconstituted mixtures of these phenolics were investigated in comparison to the original juice extracts, originating from cider and table apples. Parameters studied were (oxidative) DNA damage (Comet assay), cellular redox status (dichlorofluorescein assay) and Trolox equivalent antioxidant capacity (TEAC). The TEAC of the reconstituted mixtures was higher compared to the respective original extracts (4.7-7.3 mM vs. 3.6-4.2 mM Trolox). After 24 h cell incubation, menadione-induced (oxidative) DNA damage was more effectively reduced by the reconstituted mixtures (1-100 microg/mL, 24 h), as compared to the original extracts. In contrast, the cellular ROS level was reduced to a rather similar extent by original extracts and reconstituted mixtures. The results lead to the conclusion that the selected constituents in their authentic proportions substantially account for the antioxidative effectiveness of phenolic apple juice extracts. SN - 1613-4125 UR - https://www.unboundmedicine.com/medline/citation/16548011/Modulation_of_oxidative_cell_damage_by_reconstituted_mixtures_of_phenolic_apple_juice_extracts_in_human_colon_cell_lines_ L2 - https://doi.org/10.1002/mnfr.200500194 DB - PRIME DP - Unbound Medicine ER -