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Use of gas chromatography/mass spectrometry with positive chemical ionization for the determination of opiates in human oral fluid.
Rapid Commun Mass Spectrom. 2006; 20(8):1288-92.RC

Abstract

An analytical method for the simultaneous determination of codeine, morphine and 6-acetylmorphine (6AM) in human oral fluid was developed. The method involves liquid-liquid extraction in Toxitubes A, derivatization with 99:1 (v/v) N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)/trimethylchlorosilane (TMCS), and gas chromatography/mass spectrometry with positive chemical ionization (GC/PCI-MS) determination. The detector response was linear over the concentration range 30-500 ng/mL with coefficients of correlation higher than 0.99. The precision was acceptable with coefficients of variation less than 7.5%. The limits of detection achieved were 0.7 ng/mL for codeine, 2.0 ng/mL for morphine, and 0.6 ng/mL for 6AM. The method proposed was applied to 80 oral fluid samples from opiates users, 98% of which were positive for the three analytes. Human oral fluid is a suitable biological fluid for the determination of opiates by GC/PCI-MS.

Authors+Show Affiliations

Institute of Legal Medicine, Forensic Toxicology Service, Faculty of Medicine, University of Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16548052

Citation

Cámpora, Pamela, et al. "Use of Gas Chromatography/mass Spectrometry With Positive Chemical Ionization for the Determination of Opiates in Human Oral Fluid." Rapid Communications in Mass Spectrometry : RCM, vol. 20, no. 8, 2006, pp. 1288-92.
Cámpora P, Bermejo AM, Tabernero MJ, et al. Use of gas chromatography/mass spectrometry with positive chemical ionization for the determination of opiates in human oral fluid. Rapid Commun Mass Spectrom. 2006;20(8):1288-92.
Cámpora, P., Bermejo, A. M., Tabernero, M. J., & Fernández, P. (2006). Use of gas chromatography/mass spectrometry with positive chemical ionization for the determination of opiates in human oral fluid. Rapid Communications in Mass Spectrometry : RCM, 20(8), 1288-92.
Cámpora P, et al. Use of Gas Chromatography/mass Spectrometry With Positive Chemical Ionization for the Determination of Opiates in Human Oral Fluid. Rapid Commun Mass Spectrom. 2006;20(8):1288-92. PubMed PMID: 16548052.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of gas chromatography/mass spectrometry with positive chemical ionization for the determination of opiates in human oral fluid. AU - Cámpora,Pamela, AU - Bermejo,Ana María, AU - Tabernero,María Jesús, AU - Fernández,Purificación, PY - 2006/3/21/pubmed PY - 2007/8/7/medline PY - 2006/3/21/entrez SP - 1288 EP - 92 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 20 IS - 8 N2 - An analytical method for the simultaneous determination of codeine, morphine and 6-acetylmorphine (6AM) in human oral fluid was developed. The method involves liquid-liquid extraction in Toxitubes A, derivatization with 99:1 (v/v) N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA)/trimethylchlorosilane (TMCS), and gas chromatography/mass spectrometry with positive chemical ionization (GC/PCI-MS) determination. The detector response was linear over the concentration range 30-500 ng/mL with coefficients of correlation higher than 0.99. The precision was acceptable with coefficients of variation less than 7.5%. The limits of detection achieved were 0.7 ng/mL for codeine, 2.0 ng/mL for morphine, and 0.6 ng/mL for 6AM. The method proposed was applied to 80 oral fluid samples from opiates users, 98% of which were positive for the three analytes. Human oral fluid is a suitable biological fluid for the determination of opiates by GC/PCI-MS. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/16548052/Use_of_gas_chromatography/mass_spectrometry_with_positive_chemical_ionization_for_the_determination_of_opiates_in_human_oral_fluid_ L2 - https://doi.org/10.1002/rcm.2449 DB - PRIME DP - Unbound Medicine ER -