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Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light.
Nat Biotechnol 2006; 24(4):461-5NBio

Abstract

Green fluorescent protein (GFP) and GFP-like proteins represent invaluable genetically encoded fluorescent probes. In the last few years a new class of photoactivatable fluorescent proteins (PAFPs) capable of pronounced light-induced spectral changes have been developed. Except for tetrameric KFP1 (ref. 4), all known PAFPs, including PA-GFP, Kaede, EosFP, PS-CFP, Dronpa, PA-mRFP1 and KikGR require light in the UV-violet spectral region for activation through one-photon excitation--such light can be phototoxic to some biological systems. Here, we report a monomeric PAFP, Dendra, derived from octocoral Dendronephthya sp. and capable of 1,000- to 4,500-fold photoconversion from green to red fluorescent states in response to either visible blue or UV-violet light. Dendra represents the first PAFP, which is simultaneously monomeric, efficiently matures at 37 degrees C, demonstrates high photostability of the activated state, and can be photoactivated by a common, marginally phototoxic, 488-nm laser line. We demonstrate the suitability of Dendra for protein labeling and tracking to quantitatively study dynamics of fibrillarin and vimentin in mammalian cells.

Authors+Show Affiliations

Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16550175

Citation

Gurskaya, Nadya G., et al. "Engineering of a Monomeric Green-to-red Photoactivatable Fluorescent Protein Induced By Blue Light." Nature Biotechnology, vol. 24, no. 4, 2006, pp. 461-5.
Gurskaya NG, Verkhusha VV, Shcheglov AS, et al. Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nat Biotechnol. 2006;24(4):461-5.
Gurskaya, N. G., Verkhusha, V. V., Shcheglov, A. S., Staroverov, D. B., Chepurnykh, T. V., Fradkov, A. F., ... Lukyanov, K. A. (2006). Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. Nature Biotechnology, 24(4), pp. 461-5.
Gurskaya NG, et al. Engineering of a Monomeric Green-to-red Photoactivatable Fluorescent Protein Induced By Blue Light. Nat Biotechnol. 2006;24(4):461-5. PubMed PMID: 16550175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Engineering of a monomeric green-to-red photoactivatable fluorescent protein induced by blue light. AU - Gurskaya,Nadya G, AU - Verkhusha,Vladislav V, AU - Shcheglov,Alexander S, AU - Staroverov,Dmitry B, AU - Chepurnykh,Tatyana V, AU - Fradkov,Arkady F, AU - Lukyanov,Sergey, AU - Lukyanov,Konstantin A, Y1 - 2006/03/19/ PY - 2005/09/22/received PY - 2005/12/13/accepted PY - 2006/3/22/pubmed PY - 2006/6/30/medline PY - 2006/3/22/entrez SP - 461 EP - 5 JF - Nature biotechnology JO - Nat. Biotechnol. VL - 24 IS - 4 N2 - Green fluorescent protein (GFP) and GFP-like proteins represent invaluable genetically encoded fluorescent probes. In the last few years a new class of photoactivatable fluorescent proteins (PAFPs) capable of pronounced light-induced spectral changes have been developed. Except for tetrameric KFP1 (ref. 4), all known PAFPs, including PA-GFP, Kaede, EosFP, PS-CFP, Dronpa, PA-mRFP1 and KikGR require light in the UV-violet spectral region for activation through one-photon excitation--such light can be phototoxic to some biological systems. Here, we report a monomeric PAFP, Dendra, derived from octocoral Dendronephthya sp. and capable of 1,000- to 4,500-fold photoconversion from green to red fluorescent states in response to either visible blue or UV-violet light. Dendra represents the first PAFP, which is simultaneously monomeric, efficiently matures at 37 degrees C, demonstrates high photostability of the activated state, and can be photoactivated by a common, marginally phototoxic, 488-nm laser line. We demonstrate the suitability of Dendra for protein labeling and tracking to quantitatively study dynamics of fibrillarin and vimentin in mammalian cells. SN - 1087-0156 UR - https://www.unboundmedicine.com/medline/citation/16550175/Engineering_of_a_monomeric_green_to_red_photoactivatable_fluorescent_protein_induced_by_blue_light_ L2 - https://dx.doi.org/10.1038/nbt1191 DB - PRIME DP - Unbound Medicine ER -