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Low correspondence between K-ras mutations in pancreatic cancer tissue and detection of K-ras mutations in circulating DNA.
Pancreas. 2006 Mar; 32(2):171-7.P

Abstract

OBJECTIVE

K-ras is the most frequently mutated gene in pancreatic cancer; reported rates range from 70% to 90%. The aim of this study was to evaluate the correspondence between K-ras mutations in pancreatic cancer tissue and in circulating DNA and the value of K-ras mutations as serological marker.

METHODS

The research was conducted in 30 patients with pancreatic cancer in whom both plasma and neoplastic tissues were available. Such research was extended to circulating DNA isolated from 40 patients with chronic pancreatitis. Mutations in codon 12 were examined by mutant allele-specific amplification method and by direct sequencing. Serum values of routinely used tumor markers such as carbohydrate antigen (Ca) 19.9, carcinoembryonic antigen, Ca 50, and Ca 242 have been tested in all the patients enrolled in this study.

RESULTS

K-ras mutations were detected in 70% of neoplastic tissue samples, but no mutated DNA resulted in circulating DNA samples. The 60% of patients with tissue K-ras mutation showed elevation of some tumor markers among Ca 19.9, carcinoembryonic antigen, Ca 50, and Ca 242. As a whole, these last showed low sensitivity (20%-56.67%) and specificity (56.67%-77.5%) when compared with chronic pancreatitis.

CONCLUSION

Over the years, there has been no change in the direction of an earlier diagnosis by serological markers, and also, these data indicate that K-ras mutation in serum is an unsatisfactory method for the detection in patients with pancreatic cancer as well as in patients with high risk of progression toward neoplastic pancreatic disease.

Authors+Show Affiliations

FBF S. Pietro Hospital AFaR Research Centre, University of Rome La Sapienza, Rome, Italy. marchese.rodolfo@fbfrm.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16552337

Citation

Marchese, Rodolfo, et al. "Low Correspondence Between K-ras Mutations in Pancreatic Cancer Tissue and Detection of K-ras Mutations in Circulating DNA." Pancreas, vol. 32, no. 2, 2006, pp. 171-7.
Marchese R, Muleti A, Pasqualetti P, et al. Low correspondence between K-ras mutations in pancreatic cancer tissue and detection of K-ras mutations in circulating DNA. Pancreas. 2006;32(2):171-7.
Marchese, R., Muleti, A., Pasqualetti, P., Bucci, B., Stigliano, A., Brunetti, E., De Angelis, M., Mazzoni, G., Tocchi, A., & Brozzetti, S. (2006). Low correspondence between K-ras mutations in pancreatic cancer tissue and detection of K-ras mutations in circulating DNA. Pancreas, 32(2), 171-7.
Marchese R, et al. Low Correspondence Between K-ras Mutations in Pancreatic Cancer Tissue and Detection of K-ras Mutations in Circulating DNA. Pancreas. 2006;32(2):171-7. PubMed PMID: 16552337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Low correspondence between K-ras mutations in pancreatic cancer tissue and detection of K-ras mutations in circulating DNA. AU - Marchese,Rodolfo, AU - Muleti,Alessandra, AU - Pasqualetti,Patrizio, AU - Bucci,Barbara, AU - Stigliano,Antonio, AU - Brunetti,Ercole, AU - De Angelis,Monica, AU - Mazzoni,Gianluca, AU - Tocchi,Adriano, AU - Brozzetti,Stefania, PY - 2006/3/23/pubmed PY - 2006/6/3/medline PY - 2006/3/23/entrez SP - 171 EP - 7 JF - Pancreas JO - Pancreas VL - 32 IS - 2 N2 - OBJECTIVE: K-ras is the most frequently mutated gene in pancreatic cancer; reported rates range from 70% to 90%. The aim of this study was to evaluate the correspondence between K-ras mutations in pancreatic cancer tissue and in circulating DNA and the value of K-ras mutations as serological marker. METHODS: The research was conducted in 30 patients with pancreatic cancer in whom both plasma and neoplastic tissues were available. Such research was extended to circulating DNA isolated from 40 patients with chronic pancreatitis. Mutations in codon 12 were examined by mutant allele-specific amplification method and by direct sequencing. Serum values of routinely used tumor markers such as carbohydrate antigen (Ca) 19.9, carcinoembryonic antigen, Ca 50, and Ca 242 have been tested in all the patients enrolled in this study. RESULTS: K-ras mutations were detected in 70% of neoplastic tissue samples, but no mutated DNA resulted in circulating DNA samples. The 60% of patients with tissue K-ras mutation showed elevation of some tumor markers among Ca 19.9, carcinoembryonic antigen, Ca 50, and Ca 242. As a whole, these last showed low sensitivity (20%-56.67%) and specificity (56.67%-77.5%) when compared with chronic pancreatitis. CONCLUSION: Over the years, there has been no change in the direction of an earlier diagnosis by serological markers, and also, these data indicate that K-ras mutation in serum is an unsatisfactory method for the detection in patients with pancreatic cancer as well as in patients with high risk of progression toward neoplastic pancreatic disease. SN - 1536-4828 UR - https://www.unboundmedicine.com/medline/citation/16552337/Low_correspondence_between_K_ras_mutations_in_pancreatic_cancer_tissue_and_detection_of_K_ras_mutations_in_circulating_DNA_ L2 - https://doi.org/10.1097/01.mpa.0000202938.63084.e3 DB - PRIME DP - Unbound Medicine ER -