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Protection against cisplatin-induced nephrotoxicity by Spirulina in rats.
Cancer Chemother Pharmacol. 2006 Dec; 58(6):802-8.CC

Abstract

PURPOSE

Cisplatin (CP)-induced nephrotoxicity is associated with the increased generation of reactive oxygen metabolites and lipid peroxidation in kidney, caused by the decreased levels of antioxidants and antioxidant enzymes. The purpose of this study was to evaluate the role of Spirulina, blue-green alga with antioxidant properties, in the protection of cisplatin-induced nephrotoxicity in rat.

METHODS

Rats were treated with CP (6 mg/kg bw, single dose, intraperitoneally). Spirulina (1,000 mg/kg) was administered orally for 8 days and CP treatment was given on day 4. Nephrotoxicity was assessed, 6 days after the CP treatment, by measuring plasma urea, creatinine, urinary N-acetyl-(D-glucose-aminidase) (beta-NAG) and histopathology of kidney.

RESULTS

Rats treated with CP showed marked nephrotoxicity as evidenced from the significant elevation in plasma urea, creatinine and urinary beta-NAG. Histological assessment revealed marked proximal tubular necrosis and extensive epithelial vacuolization in the kidney of CP-treated rats. Superoxide dismutase, catalase and glutathione peroxidase were decreased and lipid peroxidation was increased in kidney tissue. Pretreatment with Spirulina protected the rats from CP-induced nephrotoxicity. The rise in plasma urea, creatinine, urinary beta-NAG, plasma and kidney tissue MDA and histomorphological changes were significantly attenuated by Spirulina. In vitro studies using human ovarian cancer cells revealed that Spirulina did not interfere with the cytotoxic effects of CP on tumor cells.

CONCLUSIONS

In summary, Spirulina significantly protected the CP-induced nephrotoxicity through its antioxidant properties.

Authors+Show Affiliations

Department of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, 500 082, Hyderabad, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16552571

Citation

Mohan, Iyyapu Krishna, et al. "Protection Against Cisplatin-induced Nephrotoxicity By Spirulina in Rats." Cancer Chemotherapy and Pharmacology, vol. 58, no. 6, 2006, pp. 802-8.
Mohan IK, Khan M, Shobha JC, et al. Protection against cisplatin-induced nephrotoxicity by Spirulina in rats. Cancer Chemother Pharmacol. 2006;58(6):802-8.
Mohan, I. K., Khan, M., Shobha, J. C., Naidu, M. U., Prayag, A., Kuppusamy, P., & Kutala, V. K. (2006). Protection against cisplatin-induced nephrotoxicity by Spirulina in rats. Cancer Chemotherapy and Pharmacology, 58(6), 802-8.
Mohan IK, et al. Protection Against Cisplatin-induced Nephrotoxicity By Spirulina in Rats. Cancer Chemother Pharmacol. 2006;58(6):802-8. PubMed PMID: 16552571.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protection against cisplatin-induced nephrotoxicity by Spirulina in rats. AU - Mohan,Iyyapu Krishna, AU - Khan,Mahmood, AU - Shobha,Jagdish Chandra, AU - Naidu,Madireddy Umamaheswara Rao, AU - Prayag,Aruna, AU - Kuppusamy,Periannan, AU - Kutala,Vijay Kumar, Y1 - 2006/03/22/ PY - 2006/01/04/received PY - 2006/03/01/accepted PY - 2006/3/23/pubmed PY - 2007/1/4/medline PY - 2006/3/23/entrez SP - 802 EP - 8 JF - Cancer chemotherapy and pharmacology JO - Cancer Chemother Pharmacol VL - 58 IS - 6 N2 - PURPOSE: Cisplatin (CP)-induced nephrotoxicity is associated with the increased generation of reactive oxygen metabolites and lipid peroxidation in kidney, caused by the decreased levels of antioxidants and antioxidant enzymes. The purpose of this study was to evaluate the role of Spirulina, blue-green alga with antioxidant properties, in the protection of cisplatin-induced nephrotoxicity in rat. METHODS: Rats were treated with CP (6 mg/kg bw, single dose, intraperitoneally). Spirulina (1,000 mg/kg) was administered orally for 8 days and CP treatment was given on day 4. Nephrotoxicity was assessed, 6 days after the CP treatment, by measuring plasma urea, creatinine, urinary N-acetyl-(D-glucose-aminidase) (beta-NAG) and histopathology of kidney. RESULTS: Rats treated with CP showed marked nephrotoxicity as evidenced from the significant elevation in plasma urea, creatinine and urinary beta-NAG. Histological assessment revealed marked proximal tubular necrosis and extensive epithelial vacuolization in the kidney of CP-treated rats. Superoxide dismutase, catalase and glutathione peroxidase were decreased and lipid peroxidation was increased in kidney tissue. Pretreatment with Spirulina protected the rats from CP-induced nephrotoxicity. The rise in plasma urea, creatinine, urinary beta-NAG, plasma and kidney tissue MDA and histomorphological changes were significantly attenuated by Spirulina. In vitro studies using human ovarian cancer cells revealed that Spirulina did not interfere with the cytotoxic effects of CP on tumor cells. CONCLUSIONS: In summary, Spirulina significantly protected the CP-induced nephrotoxicity through its antioxidant properties. SN - 0344-5704 UR - https://www.unboundmedicine.com/medline/citation/16552571/Protection_against_cisplatin_induced_nephrotoxicity_by_Spirulina_in_rats_ L2 - https://dx.doi.org/10.1007/s00280-006-0231-8 DB - PRIME DP - Unbound Medicine ER -