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Application of Multi Locus Sequence Typing to the analysis of the biodiversity of indigenous Saccharomyces cerevisiae wine yeasts from Lebanon.
J Appl Microbiol. 2006 Apr; 100(4):699-711.JA

Abstract

AIMS

To assess suitability of Multi Locus Sequence Typing (MLST) for investigating the biodiversity of wine yeast strains. This method was compared with established ones like microsatellite analysis or amplification of genomic regions flanked by repeated (delta) elements.

METHODS AND RESULTS

DNA fragments were amplified and sequenced for 26 loci representing housekeeping genes, open reading frames (ORFs) of unknown functions or intergenic regions. A set of seven loci was tested on 84 Saccharomyces cerevisiae strains, including 65 strains isolated from traditional wineries in Lebanon, commercial wine strains and Asian isolates. An overall sequence diversity of 2.05% was observed, consisting of single nucleotide polymorphisms, 60% of them occurring in a heterozygous state. The number of polymorphic sites per locus varied between 4 and 14. The same set of strains was analysed by microsatellite typing on six polymorphic loci and by interdelta amplification.

CONCLUSIONS

Clustering of MLST profiles clearly differentiated the Asian group of strains from Lebanese and European commercial strains that appear closely related. The current MLST scheme appears less discriminatory (92.27%) on closely related wine yeasts than microsatellite or interdelta typing (>99%).

SIGNIFICANCE AND IMPACT OF THE STUDY

MLST is a highly reliable method for relatedness inference and promising for wine yeast typing.

Authors+Show Affiliations

Collection de Levures d'Intérêt Biotechnologique, UMR INA-PG-INRA-CNRS Microbiologie Génétique Moléculaire, INA-PG, Thiverval-Grignon, France.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16553725

Citation

Ayoub, M-J, et al. "Application of Multi Locus Sequence Typing to the Analysis of the Biodiversity of Indigenous Saccharomyces Cerevisiae Wine Yeasts From Lebanon." Journal of Applied Microbiology, vol. 100, no. 4, 2006, pp. 699-711.
Ayoub MJ, Legras JL, Saliba R, et al. Application of Multi Locus Sequence Typing to the analysis of the biodiversity of indigenous Saccharomyces cerevisiae wine yeasts from Lebanon. J Appl Microbiol. 2006;100(4):699-711.
Ayoub, M. J., Legras, J. L., Saliba, R., & Gaillardin, C. (2006). Application of Multi Locus Sequence Typing to the analysis of the biodiversity of indigenous Saccharomyces cerevisiae wine yeasts from Lebanon. Journal of Applied Microbiology, 100(4), 699-711.
Ayoub MJ, et al. Application of Multi Locus Sequence Typing to the Analysis of the Biodiversity of Indigenous Saccharomyces Cerevisiae Wine Yeasts From Lebanon. J Appl Microbiol. 2006;100(4):699-711. PubMed PMID: 16553725.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of Multi Locus Sequence Typing to the analysis of the biodiversity of indigenous Saccharomyces cerevisiae wine yeasts from Lebanon. AU - Ayoub,M-J, AU - Legras,J-L, AU - Saliba,R, AU - Gaillardin,C, PY - 2006/3/24/pubmed PY - 2006/9/29/medline PY - 2006/3/24/entrez SP - 699 EP - 711 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 100 IS - 4 N2 - AIMS: To assess suitability of Multi Locus Sequence Typing (MLST) for investigating the biodiversity of wine yeast strains. This method was compared with established ones like microsatellite analysis or amplification of genomic regions flanked by repeated (delta) elements. METHODS AND RESULTS: DNA fragments were amplified and sequenced for 26 loci representing housekeeping genes, open reading frames (ORFs) of unknown functions or intergenic regions. A set of seven loci was tested on 84 Saccharomyces cerevisiae strains, including 65 strains isolated from traditional wineries in Lebanon, commercial wine strains and Asian isolates. An overall sequence diversity of 2.05% was observed, consisting of single nucleotide polymorphisms, 60% of them occurring in a heterozygous state. The number of polymorphic sites per locus varied between 4 and 14. The same set of strains was analysed by microsatellite typing on six polymorphic loci and by interdelta amplification. CONCLUSIONS: Clustering of MLST profiles clearly differentiated the Asian group of strains from Lebanese and European commercial strains that appear closely related. The current MLST scheme appears less discriminatory (92.27%) on closely related wine yeasts than microsatellite or interdelta typing (>99%). SIGNIFICANCE AND IMPACT OF THE STUDY: MLST is a highly reliable method for relatedness inference and promising for wine yeast typing. SN - 1364-5072 UR - https://www.unboundmedicine.com/medline/citation/16553725/Application_of_Multi_Locus_Sequence_Typing_to_the_analysis_of_the_biodiversity_of_indigenous_Saccharomyces_cerevisiae_wine_yeasts_from_Lebanon_ DB - PRIME DP - Unbound Medicine ER -