Tags

Type your tag names separated by a space and hit enter

[Immunotherapeutic efficacy of both helper T lymphocytes and cytotoxic T lymphocytes epitopes augmented dendritic cells tumor vaccine on gastric cancer].

Abstract

OBJECTIVE

To investigate the immunotherapy efficacy of both helper T lymphocytes (Th) and cytotoxic T lymphocytes (CTL) epitopes augmented dendritic cells (DCs) tumor vaccine on gastric cancer.

METHODS

Naïve spleen T cells were stimulated by mixed peptides (a mixture of Th epitope MAGE-3 (22-36)) primed DCs per week in vitro. After 4 cycles of restimulation, peptide specific T cells were harvested and subgroups of which were determined with flow cytometry. Cytokines secreting profiles by CD4+ T cells and cytotoxicities of CD8+ T cells on tumor cells were assessed. The protective immunity by referred DCs tumor vaccines was also monitored.

RESULTS

Both Th and CTL epitopes primed DCs could elicit both CD4+ T cells and CD8+ T cells in vitro,of which CD4+ T cells released high amount of Th1 type cytokines (IFN-gamma, IL-2) on recognizing specific antigen, as well as CD8+ T cells exhibited efficient tumor-killing capacity. The effects induced by DCs pulsed with single epitope (Th or CTL epitope) in vivo were less effective than those induced by DCs pulsed with mixture epitopes.

CONCLUSIONS

Both Th and CTL epitopes augmented DCs tumor vaccine can induce CD4+ Th1 and CD8+ CTL mediated immune responses to eradicate gastric cancer cells.

Authors+Show Affiliations

Department of Surgery, Ruijin Hospital, Shcool of Medicine, Shanghai Jiaotong University, Shanghai Institute of Digestive Surgery, Shanghai 200025, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

16555158

Citation

Li, Qiang, et al. "[Immunotherapeutic Efficacy of Both Helper T Lymphocytes and Cytotoxic T Lymphocytes Epitopes Augmented Dendritic Cells Tumor Vaccine On Gastric Cancer]." Zhonghua Wei Chang Wai Ke Za Zhi = Chinese Journal of Gastrointestinal Surgery, vol. 9, no. 2, 2006, pp. 148-51.
Li Q, Zhang Y, Chen XH, et al. [Immunotherapeutic efficacy of both helper T lymphocytes and cytotoxic T lymphocytes epitopes augmented dendritic cells tumor vaccine on gastric cancer]. Zhonghua Wei Chang Wai Ke Za Zhi. 2006;9(2):148-51.
Li, Q., Zhang, Y., Chen, X. H., Cao, W. X., Gu, Q. L., Zhu, Z. G., & Liu, B. Y. (2006). [Immunotherapeutic efficacy of both helper T lymphocytes and cytotoxic T lymphocytes epitopes augmented dendritic cells tumor vaccine on gastric cancer]. Zhonghua Wei Chang Wai Ke Za Zhi = Chinese Journal of Gastrointestinal Surgery, 9(2), pp. 148-51.
Li Q, et al. [Immunotherapeutic Efficacy of Both Helper T Lymphocytes and Cytotoxic T Lymphocytes Epitopes Augmented Dendritic Cells Tumor Vaccine On Gastric Cancer]. Zhonghua Wei Chang Wai Ke Za Zhi. 2006;9(2):148-51. PubMed PMID: 16555158.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Immunotherapeutic efficacy of both helper T lymphocytes and cytotoxic T lymphocytes epitopes augmented dendritic cells tumor vaccine on gastric cancer]. AU - Li,Qiang, AU - Zhang,Yi, AU - Chen,Xue-hua, AU - Cao,Wei-xin, AU - Gu,Qin-long, AU - Zhu,Zheng-gang, AU - Liu,Bing-ya, PY - 2006/3/24/pubmed PY - 2009/9/5/medline PY - 2006/3/24/entrez SP - 148 EP - 51 JF - Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery JO - Zhonghua Wei Chang Wai Ke Za Zhi VL - 9 IS - 2 N2 - OBJECTIVE: To investigate the immunotherapy efficacy of both helper T lymphocytes (Th) and cytotoxic T lymphocytes (CTL) epitopes augmented dendritic cells (DCs) tumor vaccine on gastric cancer. METHODS: Naïve spleen T cells were stimulated by mixed peptides (a mixture of Th epitope MAGE-3 (22-36)) primed DCs per week in vitro. After 4 cycles of restimulation, peptide specific T cells were harvested and subgroups of which were determined with flow cytometry. Cytokines secreting profiles by CD4+ T cells and cytotoxicities of CD8+ T cells on tumor cells were assessed. The protective immunity by referred DCs tumor vaccines was also monitored. RESULTS: Both Th and CTL epitopes primed DCs could elicit both CD4+ T cells and CD8+ T cells in vitro,of which CD4+ T cells released high amount of Th1 type cytokines (IFN-gamma, IL-2) on recognizing specific antigen, as well as CD8+ T cells exhibited efficient tumor-killing capacity. The effects induced by DCs pulsed with single epitope (Th or CTL epitope) in vivo were less effective than those induced by DCs pulsed with mixture epitopes. CONCLUSIONS: Both Th and CTL epitopes augmented DCs tumor vaccine can induce CD4+ Th1 and CD8+ CTL mediated immune responses to eradicate gastric cancer cells. SN - 1671-0274 UR - https://www.unboundmedicine.com/medline/citation/16555158/[Immunotherapeutic_efficacy_of_both_helper_T_lymphocytes_and_cytotoxic_T_lymphocytes_epitopes_augmented_dendritic_cells_tumor_vaccine_on_gastric_cancer]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=1671-0274&year=2006&vol=9&issue=2&fpage=148 DB - PRIME DP - Unbound Medicine ER -