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RNase R affects gene expression in stationary phase: regulation of ompA.
Mol Microbiol. 2006 Apr; 60(1):219-28.MM

Abstract

In nature, bacteria remain mostly in the stationary phase of the life cycle. Although mRNA is a major determinant of gene expression, little is known about mRNA decay in the stationary phase. The results presented herein demonstrate that RNase R is induced in stationary phase and is involved in the post-transcriptional regulation of ompA mRNA. This work is the first report of RNase R activity on a full length mRNA. In the absence of RNase R in a single rnr mutant, higher levels of ompA mRNA are found as a consequence of the stabilization of ompA full transcript. This effect is growth-phase-specific and not a growth-rate-dependent event. These higher levels of ompA mRNA were correlated with increases in the amounts of OmpA protein. We have also analysed the role of other factors that could affect ompA mRNA stability in stationary phase. RNase E was found to have the most important role, followed by polyadenylation. PNPase also affected the decay of the ompA transcript but RNase II did not seem to contribute much to this degradation process. The participation of RNase R in poly(A)-dependent pathways of decay in stationary phase of growth is discussed. The results show that RNase R can be a modulator of gene expression in stationary phase cells.

Authors+Show Affiliations

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16556233

Citation

Andrade, José Marques, et al. "RNase R Affects Gene Expression in Stationary Phase: Regulation of OmpA." Molecular Microbiology, vol. 60, no. 1, 2006, pp. 219-28.
Andrade JM, Cairrão F, Arraiano CM. RNase R affects gene expression in stationary phase: regulation of ompA. Mol Microbiol. 2006;60(1):219-28.
Andrade, J. M., Cairrão, F., & Arraiano, C. M. (2006). RNase R affects gene expression in stationary phase: regulation of ompA. Molecular Microbiology, 60(1), 219-28.
Andrade JM, Cairrão F, Arraiano CM. RNase R Affects Gene Expression in Stationary Phase: Regulation of OmpA. Mol Microbiol. 2006;60(1):219-28. PubMed PMID: 16556233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - RNase R affects gene expression in stationary phase: regulation of ompA. AU - Andrade,José Marques, AU - Cairrão,Fátima, AU - Arraiano,Cecília Maria, PY - 2006/3/25/pubmed PY - 2006/6/28/medline PY - 2006/3/25/entrez SP - 219 EP - 28 JF - Molecular microbiology JO - Mol Microbiol VL - 60 IS - 1 N2 - In nature, bacteria remain mostly in the stationary phase of the life cycle. Although mRNA is a major determinant of gene expression, little is known about mRNA decay in the stationary phase. The results presented herein demonstrate that RNase R is induced in stationary phase and is involved in the post-transcriptional regulation of ompA mRNA. This work is the first report of RNase R activity on a full length mRNA. In the absence of RNase R in a single rnr mutant, higher levels of ompA mRNA are found as a consequence of the stabilization of ompA full transcript. This effect is growth-phase-specific and not a growth-rate-dependent event. These higher levels of ompA mRNA were correlated with increases in the amounts of OmpA protein. We have also analysed the role of other factors that could affect ompA mRNA stability in stationary phase. RNase E was found to have the most important role, followed by polyadenylation. PNPase also affected the decay of the ompA transcript but RNase II did not seem to contribute much to this degradation process. The participation of RNase R in poly(A)-dependent pathways of decay in stationary phase of growth is discussed. The results show that RNase R can be a modulator of gene expression in stationary phase cells. SN - 0950-382X UR - https://www.unboundmedicine.com/medline/citation/16556233/RNase_R_affects_gene_expression_in_stationary_phase:_regulation_of_ompA_ L2 - https://doi.org/10.1111/j.1365-2958.2006.05092.x DB - PRIME DP - Unbound Medicine ER -