Tags

Type your tag names separated by a space and hit enter

Quantitative analysis of aromatase, sulfatase and 17beta-HSD(1) mRNA expression in soft tissue metastases of breast cancer.
Cancer Lett. 2006 Nov 08; 243(1):23-31.CL

Abstract

Expression of the estrogen-synthesizing genes aromatase, steroid sulfatase (STS) and 17beta-hydroxysteroid dehydrogenase type1 (17beta-HSD(1)) has been shown to be up-regulated in primary breast cancer tissue but their expression status in metastatic tumor tissue has yet to be determined. The mRNA expression levels of the three estrogen-synthesizing genes as well as of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and cyclooxygenase (COX)-2, all of which have been reported to up-regulate the estrogen-synthesizing genes, were determined by means of a real-time PCR assay in 100 primary breast cancer tissues and 15 soft tissue metastases. In addition, PCR-gel electrophoresis was used to determine the proportion (%) of promoter (l.4, l.3, Pll and l.7) usage of aromatase. Aromatase and STS mRNA levels were significantly (P=0.04 and P=0.03, respectively) higher in soft tissue metastases than in primary tumors, while 17beta-HSD(1) mRNA levels tended (P=0.09) to be higher. The proportions of the promoter usages were very similar for primary tumors and soft tissue metastases, and the mRNA levels of TNF-alpha, IL-6 and COX-2 were not significantly different. Levels of aromatase, STS and 17beta-HSD(1) mRNA are up-regulated in soft tissue metastases compared to those in primary tumors, suggesting that intra-tumoral estrogen synthesis may play a significant role in the growth stimulation of tumor cells in soft tissue metastases as in primary tumors. TNF-alpha, IL-6 and COX-2, on the other hand, are unlikely to be implicated in this up-regulation.

Authors+Show Affiliations

Department of Breast and Endocrine Surgery, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16556483

Citation

Irahara, Natsumi, et al. "Quantitative Analysis of Aromatase, Sulfatase and 17beta-HSD(1) mRNA Expression in Soft Tissue Metastases of Breast Cancer." Cancer Letters, vol. 243, no. 1, 2006, pp. 23-31.
Irahara N, Miyoshi Y, Taguchi T, et al. Quantitative analysis of aromatase, sulfatase and 17beta-HSD(1) mRNA expression in soft tissue metastases of breast cancer. Cancer Lett. 2006;243(1):23-31.
Irahara, N., Miyoshi, Y., Taguchi, T., Tamaki, Y., & Noguchi, S. (2006). Quantitative analysis of aromatase, sulfatase and 17beta-HSD(1) mRNA expression in soft tissue metastases of breast cancer. Cancer Letters, 243(1), 23-31.
Irahara N, et al. Quantitative Analysis of Aromatase, Sulfatase and 17beta-HSD(1) mRNA Expression in Soft Tissue Metastases of Breast Cancer. Cancer Lett. 2006 Nov 8;243(1):23-31. PubMed PMID: 16556483.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative analysis of aromatase, sulfatase and 17beta-HSD(1) mRNA expression in soft tissue metastases of breast cancer. AU - Irahara,Natsumi, AU - Miyoshi,Yasuo, AU - Taguchi,Tetsuya, AU - Tamaki,Yasuhiro, AU - Noguchi,Shinzaburo, Y1 - 2006/03/23/ PY - 2005/09/09/received PY - 2005/11/09/revised PY - 2005/11/09/accepted PY - 2006/3/25/pubmed PY - 2006/12/9/medline PY - 2006/3/25/entrez SP - 23 EP - 31 JF - Cancer letters JO - Cancer Lett VL - 243 IS - 1 N2 - Expression of the estrogen-synthesizing genes aromatase, steroid sulfatase (STS) and 17beta-hydroxysteroid dehydrogenase type1 (17beta-HSD(1)) has been shown to be up-regulated in primary breast cancer tissue but their expression status in metastatic tumor tissue has yet to be determined. The mRNA expression levels of the three estrogen-synthesizing genes as well as of tumor necrosis factor (TNF)-alpha, interleukin (IL)-6 and cyclooxygenase (COX)-2, all of which have been reported to up-regulate the estrogen-synthesizing genes, were determined by means of a real-time PCR assay in 100 primary breast cancer tissues and 15 soft tissue metastases. In addition, PCR-gel electrophoresis was used to determine the proportion (%) of promoter (l.4, l.3, Pll and l.7) usage of aromatase. Aromatase and STS mRNA levels were significantly (P=0.04 and P=0.03, respectively) higher in soft tissue metastases than in primary tumors, while 17beta-HSD(1) mRNA levels tended (P=0.09) to be higher. The proportions of the promoter usages were very similar for primary tumors and soft tissue metastases, and the mRNA levels of TNF-alpha, IL-6 and COX-2 were not significantly different. Levels of aromatase, STS and 17beta-HSD(1) mRNA are up-regulated in soft tissue metastases compared to those in primary tumors, suggesting that intra-tumoral estrogen synthesis may play a significant role in the growth stimulation of tumor cells in soft tissue metastases as in primary tumors. TNF-alpha, IL-6 and COX-2, on the other hand, are unlikely to be implicated in this up-regulation. SN - 0304-3835 UR - https://www.unboundmedicine.com/medline/citation/16556483/Quantitative_analysis_of_aromatase_sulfatase_and_17beta_HSD_1__mRNA_expression_in_soft_tissue_metastases_of_breast_cancer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3835(05)00993-6 DB - PRIME DP - Unbound Medicine ER -