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Phorbol ester-mediated inhibition of IFN-alpha/beta gene transcription in blood mononuclear leukocytes.
J Immunol 1991; 147(9):3116-21JI

Abstract

The phorbol ester PMA efficiently inhibits the in vitro IFN-alpha and -beta responses in human blood monocytes induced by Sendai virus and in natural IFN-producing cells induced by glutaraldehyde-fixed herpes simplex virus-infected human amnion (WISH) cells. This PMA-mediated inhibition of IFN-alpha/beta secretion is correlated with considerably reduced levels of IFN-alpha/beta mRNA, as determined by Northern blot analysis. Nuclear run-on assays further show that, at least in monocytes, PMA inhibits transcription of the IFN-alpha and -beta genes. The synthetic diacylglycerol 1-oleoyl-2-acetylglycerol has the same effects as PMA, and the protein kinase inhibitor staurosporine prevents the PMA-mediated inhibition of IFN-alpha/beta expression. These results suggest a role for protein kinase C in the inhibition of IFN-alpha/beta responses. The PMA does not inhibit the accumulation of IFN-beta mRNA in monocytes stimulated by Sendai virus in the presence of cycloheximide, indicating that the inhibitory action of the phorbol ester requires de novo protein synthesis.

Authors+Show Affiliations

Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1655906

Citation

Sandberg, K, et al. "Phorbol Ester-mediated Inhibition of IFN-alpha/beta Gene Transcription in Blood Mononuclear Leukocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 147, no. 9, 1991, pp. 3116-21.
Sandberg K, Eloranta ML, Gobl AE, et al. Phorbol ester-mediated inhibition of IFN-alpha/beta gene transcription in blood mononuclear leukocytes. J Immunol. 1991;147(9):3116-21.
Sandberg, K., Eloranta, M. L., Gobl, A. E., & Alm, G. V. (1991). Phorbol ester-mediated inhibition of IFN-alpha/beta gene transcription in blood mononuclear leukocytes. Journal of Immunology (Baltimore, Md. : 1950), 147(9), pp. 3116-21.
Sandberg K, et al. Phorbol Ester-mediated Inhibition of IFN-alpha/beta Gene Transcription in Blood Mononuclear Leukocytes. J Immunol. 1991 Nov 1;147(9):3116-21. PubMed PMID: 1655906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phorbol ester-mediated inhibition of IFN-alpha/beta gene transcription in blood mononuclear leukocytes. AU - Sandberg,K, AU - Eloranta,M L, AU - Gobl,A E, AU - Alm,G V, PY - 1991/11/1/pubmed PY - 1991/11/1/medline PY - 1991/11/1/entrez SP - 3116 EP - 21 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 147 IS - 9 N2 - The phorbol ester PMA efficiently inhibits the in vitro IFN-alpha and -beta responses in human blood monocytes induced by Sendai virus and in natural IFN-producing cells induced by glutaraldehyde-fixed herpes simplex virus-infected human amnion (WISH) cells. This PMA-mediated inhibition of IFN-alpha/beta secretion is correlated with considerably reduced levels of IFN-alpha/beta mRNA, as determined by Northern blot analysis. Nuclear run-on assays further show that, at least in monocytes, PMA inhibits transcription of the IFN-alpha and -beta genes. The synthetic diacylglycerol 1-oleoyl-2-acetylglycerol has the same effects as PMA, and the protein kinase inhibitor staurosporine prevents the PMA-mediated inhibition of IFN-alpha/beta expression. These results suggest a role for protein kinase C in the inhibition of IFN-alpha/beta responses. The PMA does not inhibit the accumulation of IFN-beta mRNA in monocytes stimulated by Sendai virus in the presence of cycloheximide, indicating that the inhibitory action of the phorbol ester requires de novo protein synthesis. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/1655906/Phorbol_ester_mediated_inhibition_of_IFN_alpha/beta_gene_transcription_in_blood_mononuclear_leukocytes_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=1655906 DB - PRIME DP - Unbound Medicine ER -