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Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material.
J Chromatogr A. 2006 Jul 07; 1120(1-2):221-9.JC

Abstract

The antioxidant activity of rosemary (Rosmarinus officinalis) extract from different raw materials has been studied. Extracts were prepared from wild or drip-irrigated plants, as well as from the by-product resulting from the distillation of the aromatic essential oil. The radical scavenging activity of rosemary extracts was compared with that of antioxidants widely used in food, such as BHT and delta-tocopherol, using an optimization of the method based on the reduction of the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results pointed the excellent antioxidant activity of the crude fresh rosemary extracts, which was almost identical to that of pure delta-tocopherol, and higher than that of BHT; extracts prepared from distilled rosemary showed the lowest activity, although they are also of interest due to the low cost of the raw material. High performance liquid chromatography (HPLC) combined with diode array (DAD) and electrospray (ESI)-ion trap-MS detection was used to separate and identify the compounds present in the rosemary extracts. Rosmarinic acid, carnosic acid and seven of their terpene-type metabolites, and seven flavones were identified. The drying and/or distillation treatments used with the plant material strongly affected the content of the two compounds of higher antioxidant activity: rosmarinic acid and carnosic acid.

Authors+Show Affiliations

Department of Agricultural Chemistry, University of Murcia, 30100 Murcia, Spain. almela@um.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16563403

Citation

Almela, Luis, et al. "Liquid Chromatograpic-mass Spectrometric Analysis of Phenolics and Free Radical Scavenging Activity of Rosemary Extract From Different Raw Material." Journal of Chromatography. A, vol. 1120, no. 1-2, 2006, pp. 221-9.
Almela L, Sánchez-Muñoz B, Fernández-López JA, et al. Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material. J Chromatogr A. 2006;1120(1-2):221-9.
Almela, L., Sánchez-Muñoz, B., Fernández-López, J. A., Roca, M. J., & Rabe, V. (2006). Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material. Journal of Chromatography. A, 1120(1-2), 221-9.
Almela L, et al. Liquid Chromatograpic-mass Spectrometric Analysis of Phenolics and Free Radical Scavenging Activity of Rosemary Extract From Different Raw Material. J Chromatogr A. 2006 Jul 7;1120(1-2):221-9. PubMed PMID: 16563403.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material. AU - Almela,Luis, AU - Sánchez-Muñoz,Blas, AU - Fernández-López,José A, AU - Roca,María J, AU - Rabe,Virginia, Y1 - 2006/03/24/ PY - 2005/07/26/received PY - 2006/02/14/revised PY - 2006/02/20/accepted PY - 2006/3/28/pubmed PY - 2006/8/19/medline PY - 2006/3/28/entrez SP - 221 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1120 IS - 1-2 N2 - The antioxidant activity of rosemary (Rosmarinus officinalis) extract from different raw materials has been studied. Extracts were prepared from wild or drip-irrigated plants, as well as from the by-product resulting from the distillation of the aromatic essential oil. The radical scavenging activity of rosemary extracts was compared with that of antioxidants widely used in food, such as BHT and delta-tocopherol, using an optimization of the method based on the reduction of the radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results pointed the excellent antioxidant activity of the crude fresh rosemary extracts, which was almost identical to that of pure delta-tocopherol, and higher than that of BHT; extracts prepared from distilled rosemary showed the lowest activity, although they are also of interest due to the low cost of the raw material. High performance liquid chromatography (HPLC) combined with diode array (DAD) and electrospray (ESI)-ion trap-MS detection was used to separate and identify the compounds present in the rosemary extracts. Rosmarinic acid, carnosic acid and seven of their terpene-type metabolites, and seven flavones were identified. The drying and/or distillation treatments used with the plant material strongly affected the content of the two compounds of higher antioxidant activity: rosmarinic acid and carnosic acid. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16563403/Liquid_chromatograpic_mass_spectrometric_analysis_of_phenolics_and_free_radical_scavenging_activity_of_rosemary_extract_from_different_raw_material_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)00446-8 DB - PRIME DP - Unbound Medicine ER -