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Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection.
J Chromatogr A. 2006 Apr 28; 1113(1-2):251-4.JC

Abstract

In this paper, we present a universal, highly efficient and sensitive method for the characterization of quantum dot (QD) bioconjugates based on capillary electrophoresis with laser-induced fluorescent (LIF) detection. We first prepared CdTe QDs in aqueous phase by a chemical route with mercaptopropionic acid as a ligand, and then were coupled to certain proteins using bifunctional linkage reagent or electrostatic attraction. The QD bioconjugates were characterized by capillary electrophoresis with LIF detection. We found that QD bioconjugates were efficiently separated with free QDs by the optimization of buffer pH. Furthermore, we found that ultrafiltration was an effective and simple approach to purify QD conjugates with bovine serum albumin (BSA). Due to their broad absorption spectra and size dependent emission wavelength tunability, QDs can be excited to emit different colour fluorescence using a single wavelength laser source, and therefore, we believe that CE with LIF detection will become a universal and efficient tool for the characterization of QD bioconjugates.

Authors+Show Affiliations

College of Chemistry & Chemical Engineering, Shanghai Jiaotong University, 800 Dongchuan Road, Shanghai 200240, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16563405

Citation

Huang, Xiangyi, et al. "Characterization of Quantum Dot Bioconjugates By Capillary Electrophoresis With Laser-induced Fluorescent Detection." Journal of Chromatography. A, vol. 1113, no. 1-2, 2006, pp. 251-4.
Huang X, Weng J, Sang F, et al. Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection. J Chromatogr A. 2006;1113(1-2):251-4.
Huang, X., Weng, J., Sang, F., Song, X., Cao, C., & Ren, J. (2006). Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection. Journal of Chromatography. A, 1113(1-2), 251-4.
Huang X, et al. Characterization of Quantum Dot Bioconjugates By Capillary Electrophoresis With Laser-induced Fluorescent Detection. J Chromatogr A. 2006 Apr 28;1113(1-2):251-4. PubMed PMID: 16563405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of quantum dot bioconjugates by capillary electrophoresis with laser-induced fluorescent detection. AU - Huang,Xiangyi, AU - Weng,Jifang, AU - Sang,Fuming, AU - Song,Xingtao, AU - Cao,Chengxi, AU - Ren,Jicun, Y1 - 2006/03/24/ PY - 2005/12/28/received PY - 2006/02/16/revised PY - 2006/02/20/accepted PY - 2006/3/28/pubmed PY - 2006/7/11/medline PY - 2006/3/28/entrez SP - 251 EP - 4 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1113 IS - 1-2 N2 - In this paper, we present a universal, highly efficient and sensitive method for the characterization of quantum dot (QD) bioconjugates based on capillary electrophoresis with laser-induced fluorescent (LIF) detection. We first prepared CdTe QDs in aqueous phase by a chemical route with mercaptopropionic acid as a ligand, and then were coupled to certain proteins using bifunctional linkage reagent or electrostatic attraction. The QD bioconjugates were characterized by capillary electrophoresis with LIF detection. We found that QD bioconjugates were efficiently separated with free QDs by the optimization of buffer pH. Furthermore, we found that ultrafiltration was an effective and simple approach to purify QD conjugates with bovine serum albumin (BSA). Due to their broad absorption spectra and size dependent emission wavelength tunability, QDs can be excited to emit different colour fluorescence using a single wavelength laser source, and therefore, we believe that CE with LIF detection will become a universal and efficient tool for the characterization of QD bioconjugates. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16563405/Characterization_of_quantum_dot_bioconjugates_by_capillary_electrophoresis_with_laser_induced_fluorescent_detection_ DB - PRIME DP - Unbound Medicine ER -
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