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Different ratios of eicosapentaenoic and docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice.
J Nutr Biochem. 2007 Jan; 18(1):23-30.JN

Abstract

The use of fish oil (FO) as a dietary supplement to prevent or reduce the severity of cardiovascular diseases and autoimmune disorders such as rheumatoid arthritis is receiving much attention. Several recent reports indicate that eating fish often or the use of small doses of FO capsules appears to have benefits against cardiovascular diseases. We have reported in the past that diets enriched with FO protect against renal diseases and prolong the life span of autoimmune-prone mice compared to corn oil (CO) diets. However, the optimum ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) in commercially available FOs to reduce the production of various pro-inflammatory cytokines has not been well established. We, therefore, obtained deodorized FO from three sources containing different EPA/DHA contents, fed them to C57BL/6 mice for 8 weeks in a 10% (vol/wt) diet (oil A, 11/10; oil B, 14/9; oil C, 23/14) and compared them with (10%) CO-fed mice as control. TNF-alpha, IL-6 and IL-1beta were measured by enzyme-linked immunosorbent assay in thioglycollate-induced macrophages, 8 and 24 h after lipopolysaccharide treatment. The results showed a significant decrease in TNF-alpha after only 8 h in oil C. After 24 h, TNF-alpha, IL-6 and IL-1beta levels decreased only in mice fed oil C, although nonsignificant decreases were seen in mice fed oil A compared to mice fed CO. The antioxidant enzymes, catalase and glutathione transferase, were higher in kidneys of mice fed oil C compared to mice fed CO. The study suggests that anti-inflammatory activity may vary among different sources of FO due to variations in EPA/DHA content.

Authors+Show Affiliations

Bhattacharya A
Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229-3900, USA.
Sun D
No affiliation info available
Rahman M
No affiliation info available
Fernandes G
No affiliation info available

MeSH

AnimalsCatalaseCytokinesDietary Fats, UnsaturatedDocosahexaenoic AcidsEicosapentaenoic AcidFatty Acids, Omega-3FemaleFish OilsGlutathione PeroxidaseGlutathione TransferaseInterleukin-1betaInterleukin-6KidneyLipid PeroxidationLipopolysaccharidesMacrophages, PeritonealMiceMice, Inbred C57BLTumor Necrosis Factor-alpha

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16563716

Citation

Bhattacharya, Arunabh, et al. "Different Ratios of Eicosapentaenoic and Docosahexaenoic Omega-3 Fatty Acids in Commercial Fish Oils Differentially Alter Pro-inflammatory Cytokines in Peritoneal Macrophages From C57BL/6 Female Mice." The Journal of Nutritional Biochemistry, vol. 18, no. 1, 2007, pp. 23-30.
Bhattacharya A, Sun D, Rahman M, et al. Different ratios of eicosapentaenoic and docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice. J Nutr Biochem. 2007;18(1):23-30.
Bhattacharya, A., Sun, D., Rahman, M., & Fernandes, G. (2007). Different ratios of eicosapentaenoic and docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice. The Journal of Nutritional Biochemistry, 18(1), 23-30.
Bhattacharya A, et al. Different Ratios of Eicosapentaenoic and Docosahexaenoic Omega-3 Fatty Acids in Commercial Fish Oils Differentially Alter Pro-inflammatory Cytokines in Peritoneal Macrophages From C57BL/6 Female Mice. J Nutr Biochem. 2007;18(1):23-30. PubMed PMID: 16563716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different ratios of eicosapentaenoic and docosahexaenoic omega-3 fatty acids in commercial fish oils differentially alter pro-inflammatory cytokines in peritoneal macrophages from C57BL/6 female mice. AU - Bhattacharya,Arunabh, AU - Sun,Dongxu, AU - Rahman,Mizanur, AU - Fernandes,Gabriel, Y1 - 2006/03/24/ PY - 2005/12/29/received PY - 2006/02/15/revised PY - 2006/02/17/accepted PY - 2006/3/28/pubmed PY - 2007/3/7/medline PY - 2006/3/28/entrez SP - 23 EP - 30 JF - The Journal of nutritional biochemistry JO - J Nutr Biochem VL - 18 IS - 1 N2 - The use of fish oil (FO) as a dietary supplement to prevent or reduce the severity of cardiovascular diseases and autoimmune disorders such as rheumatoid arthritis is receiving much attention. Several recent reports indicate that eating fish often or the use of small doses of FO capsules appears to have benefits against cardiovascular diseases. We have reported in the past that diets enriched with FO protect against renal diseases and prolong the life span of autoimmune-prone mice compared to corn oil (CO) diets. However, the optimum ratio of eicosapentaenoic acid (EPA) to docosahexaenoic acid (DHA) in commercially available FOs to reduce the production of various pro-inflammatory cytokines has not been well established. We, therefore, obtained deodorized FO from three sources containing different EPA/DHA contents, fed them to C57BL/6 mice for 8 weeks in a 10% (vol/wt) diet (oil A, 11/10; oil B, 14/9; oil C, 23/14) and compared them with (10%) CO-fed mice as control. TNF-alpha, IL-6 and IL-1beta were measured by enzyme-linked immunosorbent assay in thioglycollate-induced macrophages, 8 and 24 h after lipopolysaccharide treatment. The results showed a significant decrease in TNF-alpha after only 8 h in oil C. After 24 h, TNF-alpha, IL-6 and IL-1beta levels decreased only in mice fed oil C, although nonsignificant decreases were seen in mice fed oil A compared to mice fed CO. The antioxidant enzymes, catalase and glutathione transferase, were higher in kidneys of mice fed oil C compared to mice fed CO. The study suggests that anti-inflammatory activity may vary among different sources of FO due to variations in EPA/DHA content. SN - 0955-2863 UR - https://www.unboundmedicine.com/medline/citation/16563716/Different_ratios_of_eicosapentaenoic_and_docosahexaenoic_omega_3_fatty_acids_in_commercial_fish_oils_differentially_alter_pro_inflammatory_cytokines_in_peritoneal_macrophages_from_C57BL/6_female_mice_ DB - PRIME DP - Unbound Medicine ER -