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15-Methoxypinusolidic acid from Biota orientalis attenuates glutamate-induced neurotoxicity in primary cultured rat cortical cells.
Toxicol In Vitro. 2006 Sep; 20(6):936-41.TV

Abstract

15-Methoxypinusolidic acid (15-MPA), a pinusolide derivative isolated from Biota orientalis (Cupressaceae) leaves prevented glutamate-induced excitotoxicity in primary cultured rat cortical cells in vitro. 15-MPA had more selectivity in protecting neurons against N-methyl-D-aspartate (NMDA)-induced neurotoxicity than that induced by kainic acid (KA). The glutamate-induced increase of intracellular calcium ([Ca2+]i) in cortical cells was effectively reduced by 15-MPA. Moreover, 15-MPA could successfully reduce the subsequent overproduction of nitric oxide (NO) and the level of cellular peroxide, and inhibit glutathione (GSH) depletion and lipid peroxidation induced by glutamate in our cultures. Collectively, these results suggested that 15-MPA attenuated glutamate-induced excitotoxicity via stabilization of [Ca2+]i homeostasis and suppression of oxidative stress possibly through the actions on the NMDA receptors.

Authors+Show Affiliations

Koo KA
College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, San 56-1, Shillim-Dong, Gwanak-Gu, Seoul 151-742, Republic of Korea.
Kim SH
No affiliation info available
Lee MK
No affiliation info available
Kim YC
No affiliation info available

MeSH

AnimalsCalciumCells, CulturedCerebral CortexDiterpenesGlutamic AcidGlutathioneLipid PeroxidationNeuroprotective AgentsNitric OxideOxidative StressRatsRats, Sprague-DawleyThuja

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16564156

Citation

Koo, Kyung Ah, et al. "15-Methoxypinusolidic Acid From Biota Orientalis Attenuates Glutamate-induced Neurotoxicity in Primary Cultured Rat Cortical Cells." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 20, no. 6, 2006, pp. 936-41.
Koo KA, Kim SH, Lee MK, et al. 15-Methoxypinusolidic acid from Biota orientalis attenuates glutamate-induced neurotoxicity in primary cultured rat cortical cells. Toxicol In Vitro. 2006;20(6):936-41.
Koo, K. A., Kim, S. H., Lee, M. K., & Kim, Y. C. (2006). 15-Methoxypinusolidic acid from Biota orientalis attenuates glutamate-induced neurotoxicity in primary cultured rat cortical cells. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 20(6), 936-41.
Koo KA, et al. 15-Methoxypinusolidic Acid From Biota Orientalis Attenuates Glutamate-induced Neurotoxicity in Primary Cultured Rat Cortical Cells. Toxicol In Vitro. 2006;20(6):936-41. PubMed PMID: 16564156.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 15-Methoxypinusolidic acid from Biota orientalis attenuates glutamate-induced neurotoxicity in primary cultured rat cortical cells. AU - Koo,Kyung Ah, AU - Kim,Seung Hyun, AU - Lee,Mi Kyeong, AU - Kim,Young Choong, Y1 - 2006/02/28/ PY - 2005/09/07/received PY - 2005/12/29/revised PY - 2006/02/06/accepted PY - 2006/3/28/pubmed PY - 2006/10/31/medline PY - 2006/3/28/entrez SP - 936 EP - 41 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 20 IS - 6 N2 - 15-Methoxypinusolidic acid (15-MPA), a pinusolide derivative isolated from Biota orientalis (Cupressaceae) leaves prevented glutamate-induced excitotoxicity in primary cultured rat cortical cells in vitro. 15-MPA had more selectivity in protecting neurons against N-methyl-D-aspartate (NMDA)-induced neurotoxicity than that induced by kainic acid (KA). The glutamate-induced increase of intracellular calcium ([Ca2+]i) in cortical cells was effectively reduced by 15-MPA. Moreover, 15-MPA could successfully reduce the subsequent overproduction of nitric oxide (NO) and the level of cellular peroxide, and inhibit glutathione (GSH) depletion and lipid peroxidation induced by glutamate in our cultures. Collectively, these results suggested that 15-MPA attenuated glutamate-induced excitotoxicity via stabilization of [Ca2+]i homeostasis and suppression of oxidative stress possibly through the actions on the NMDA receptors. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/16564156/15_Methoxypinusolidic_acid_from_Biota_orientalis_attenuates_glutamate_induced_neurotoxicity_in_primary_cultured_rat_cortical_cells_ DB - PRIME DP - Unbound Medicine ER -