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Deletion of the neuropeptide Y Y1 receptor affects pain sensitivity, neuropeptide transport and expression, and dorsal root ganglion neuron numbers.
Neuroscience. 2006 Jun 19; 140(1):293-304.N

Abstract

Neuropeptide Y has been implicated in pain modulation and is substantially up-regulated in dorsal root ganglia after peripheral nerve injury. To identify the role of neuropeptide Y after axotomy, we investigated the behavioral and neurochemical phenotype of neuropeptide Y Y1 receptor knockout mice with focus on dorsal root ganglion neurons and spinal cord. Using a specific antibody Y1 receptor immunoreactivity was found in dorsal root ganglia and in dorsal horn neurons of wild-type, but not knockout mice. The Y1 receptor knockout mice exhibited a pronounced mechanical hypersensitivity. After sciatic nerve axotomy, the deletion of Y1 receptor protected knockout mice from the axotomy-induced loss of dorsal root ganglion neurons seen in wild-type mice. Lower levels of calcitonin gene-related peptide and substance P were identified by immunohistochemistry in dorsal root ganglia and dorsal horn of knockout mice, and the axotomy-induced down-regulation of both calcitonin gene-related peptide and substance P was accentuated in Y1 receptor knockout. However, the transcript levels for calcitonin gene-related peptide and substance P were significantly higher in knockout than in wild-type dorsal root ganglia ipsilateral to the axotomy, while more calcitonin gene-related peptide- and substance P-like immunoreactivity accumulated proximal and distal to a crush of the sciatic nerve. These results indicate that the deletion of the Y1 receptor causes increased release and compensatory increased synthesis of calcitonin gene-related peptide and substance P in dorsal root ganglion neurons. Together, these findings suggest that, after peripheral nerve injury, neuropeptide Y, via its Y1 receptor receptor, plays a key role in cell survival as well as in transport and synthesis of the excitatory dorsal horn messengers calcitonin gene-related peptide and substance P and thus may contribute to pain hypersensitivity.

Authors+Show Affiliations

Department of Neuroscience, Karolinska Institutet, SE-171 77 Stockholm, Sweden. Tiejun.Shi@ki.seNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16564642

Citation

Shi, T-J S., et al. "Deletion of the Neuropeptide Y Y1 Receptor Affects Pain Sensitivity, Neuropeptide Transport and Expression, and Dorsal Root Ganglion Neuron Numbers." Neuroscience, vol. 140, no. 1, 2006, pp. 293-304.
Shi TJ, Li J, Dahlström A, et al. Deletion of the neuropeptide Y Y1 receptor affects pain sensitivity, neuropeptide transport and expression, and dorsal root ganglion neuron numbers. Neuroscience. 2006;140(1):293-304.
Shi, T. J., Li, J., Dahlström, A., Theodorsson, E., Ceccatelli, S., Decosterd, I., Pedrazzini, T., & Hökfelt, T. (2006). Deletion of the neuropeptide Y Y1 receptor affects pain sensitivity, neuropeptide transport and expression, and dorsal root ganglion neuron numbers. Neuroscience, 140(1), 293-304.
Shi TJ, et al. Deletion of the Neuropeptide Y Y1 Receptor Affects Pain Sensitivity, Neuropeptide Transport and Expression, and Dorsal Root Ganglion Neuron Numbers. Neuroscience. 2006 Jun 19;140(1):293-304. PubMed PMID: 16564642.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deletion of the neuropeptide Y Y1 receptor affects pain sensitivity, neuropeptide transport and expression, and dorsal root ganglion neuron numbers. AU - Shi,T-J S, AU - Li,J, AU - Dahlström,A, AU - Theodorsson,E, AU - Ceccatelli,S, AU - Decosterd,I, AU - Pedrazzini,T, AU - Hökfelt,T, Y1 - 2006/03/29/ PY - 2005/12/20/received PY - 2006/02/03/revised PY - 2006/02/04/accepted PY - 2006/3/28/pubmed PY - 2006/8/11/medline PY - 2006/3/28/entrez SP - 293 EP - 304 JF - Neuroscience JO - Neuroscience VL - 140 IS - 1 N2 - Neuropeptide Y has been implicated in pain modulation and is substantially up-regulated in dorsal root ganglia after peripheral nerve injury. To identify the role of neuropeptide Y after axotomy, we investigated the behavioral and neurochemical phenotype of neuropeptide Y Y1 receptor knockout mice with focus on dorsal root ganglion neurons and spinal cord. Using a specific antibody Y1 receptor immunoreactivity was found in dorsal root ganglia and in dorsal horn neurons of wild-type, but not knockout mice. The Y1 receptor knockout mice exhibited a pronounced mechanical hypersensitivity. After sciatic nerve axotomy, the deletion of Y1 receptor protected knockout mice from the axotomy-induced loss of dorsal root ganglion neurons seen in wild-type mice. Lower levels of calcitonin gene-related peptide and substance P were identified by immunohistochemistry in dorsal root ganglia and dorsal horn of knockout mice, and the axotomy-induced down-regulation of both calcitonin gene-related peptide and substance P was accentuated in Y1 receptor knockout. However, the transcript levels for calcitonin gene-related peptide and substance P were significantly higher in knockout than in wild-type dorsal root ganglia ipsilateral to the axotomy, while more calcitonin gene-related peptide- and substance P-like immunoreactivity accumulated proximal and distal to a crush of the sciatic nerve. These results indicate that the deletion of the Y1 receptor causes increased release and compensatory increased synthesis of calcitonin gene-related peptide and substance P in dorsal root ganglion neurons. Together, these findings suggest that, after peripheral nerve injury, neuropeptide Y, via its Y1 receptor receptor, plays a key role in cell survival as well as in transport and synthesis of the excitatory dorsal horn messengers calcitonin gene-related peptide and substance P and thus may contribute to pain hypersensitivity. SN - 0306-4522 UR - https://www.unboundmedicine.com/medline/citation/16564642/Deletion_of_the_neuropeptide_Y_Y1_receptor_affects_pain_sensitivity_neuropeptide_transport_and_expression_and_dorsal_root_ganglion_neuron_numbers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(06)00199-0 DB - PRIME DP - Unbound Medicine ER -