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DNA annealing mediated by Rad52 and Rad59 proteins.
J Biol Chem. 2006 Jun 02; 281(22):15441-9.JB

Abstract

In the budding yeast Saccharomyces cerevisiae, the RAD52 gene is essential for all homologous recombination events and its homologue, the RAD59 gene, is important for those that occur independently of RAD51. Both Rad52 and Rad59 proteins can anneal complementary single-stranded (ss) DNA. We quantitatively examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties. First, and most importantly, they differ in their ability to anneal ssDNA that is complexed with replication protein A (RPA). Rad52 can anneal an RPA-ssDNA complex, but Rad59 cannot. Second, Rad59-promoted DNA annealing follows first-order reaction kinetics, whereas Rad52-promoted annealing follows second-order reaction kinetics. Last, Rad59 enhances Rad52-mediated DNA annealing at increased NaCl concentrations, both in the absence and presence of RPA. These results suggest that Rad59 performs different functions in the recombination process, and should be more accurately viewed as a Rad52 paralogue.

Authors+Show Affiliations

Section of Microbiology, Center for Genetics and Development, Division of Biological Sciences, University of California, Davis, California 95616-8665, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

16565518

Citation

Wu, Yun, et al. "DNA Annealing Mediated By Rad52 and Rad59 Proteins." The Journal of Biological Chemistry, vol. 281, no. 22, 2006, pp. 15441-9.
Wu Y, Sugiyama T, Kowalczykowski SC. DNA annealing mediated by Rad52 and Rad59 proteins. J Biol Chem. 2006;281(22):15441-9.
Wu, Y., Sugiyama, T., & Kowalczykowski, S. C. (2006). DNA annealing mediated by Rad52 and Rad59 proteins. The Journal of Biological Chemistry, 281(22), 15441-9.
Wu Y, Sugiyama T, Kowalczykowski SC. DNA Annealing Mediated By Rad52 and Rad59 Proteins. J Biol Chem. 2006 Jun 2;281(22):15441-9. PubMed PMID: 16565518.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA annealing mediated by Rad52 and Rad59 proteins. AU - Wu,Yun, AU - Sugiyama,Tomohiko, AU - Kowalczykowski,Stephen C, Y1 - 2006/03/25/ PY - 2006/3/28/pubmed PY - 2006/8/8/medline PY - 2006/3/28/entrez SP - 15441 EP - 9 JF - The Journal of biological chemistry JO - J Biol Chem VL - 281 IS - 22 N2 - In the budding yeast Saccharomyces cerevisiae, the RAD52 gene is essential for all homologous recombination events and its homologue, the RAD59 gene, is important for those that occur independently of RAD51. Both Rad52 and Rad59 proteins can anneal complementary single-stranded (ss) DNA. We quantitatively examined the ssDNA annealing activity of Rad52 and Rad59 proteins and found significant differences in their biochemical properties. First, and most importantly, they differ in their ability to anneal ssDNA that is complexed with replication protein A (RPA). Rad52 can anneal an RPA-ssDNA complex, but Rad59 cannot. Second, Rad59-promoted DNA annealing follows first-order reaction kinetics, whereas Rad52-promoted annealing follows second-order reaction kinetics. Last, Rad59 enhances Rad52-mediated DNA annealing at increased NaCl concentrations, both in the absence and presence of RPA. These results suggest that Rad59 performs different functions in the recombination process, and should be more accurately viewed as a Rad52 paralogue. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/16565518/DNA_annealing_mediated_by_Rad52_and_Rad59_proteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(20)55992-9 DB - PRIME DP - Unbound Medicine ER -