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Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription.
Gene. 2006 Jun 07; 374:87-95.GENE

Abstract

Endothelial Differentiation Factor (EDF)-1 is a calmodulin binding protein involved in the repression of endothelial cell differentiation, a crucial, late step in angiogenesis. Its expression is cell cycle regulated, although its transcriptional regulation is yet to be determined. To map the promoter region and to understand its regulation, we cloned and fused 2300 bp upstream of EDF-1 translational start site to a luciferase reporter gene. After transient transfection in HeLa cells, this fragment was shown to possess a promoter activity. Deletion constructs of the 5' flanking region of EDF-1 lead to the identification of the minimal promoter region which was highly homologous to the mouse sequence. No TATA box was detected, whereas three consensus sequences--two GC boxes and a CAAT box--were identified. EMSA supershift and chromatin immunoprecipitation demonstrated that these sequences were binding sites for Sp1/Sp3 and NFY, respectively. Deletion of Sp1/Sp3 and NF-Y consensus sequences resulted in the total loss of EDF-1 promoter activity. Our studies indicate that Sp1 and NFY binding is essential for EDF-1 promoter activity.

Authors+Show Affiliations

University of Milan, Dipartimento di Scienze Precliniche-LITA Vialba, Via GB Grassi 74, Milan, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16567061

Citation

Bolognese, Fabrizio, et al. "Characterization of the Human EDF-1 Minimal Promoter: Involvement of NFY and Sp1 in the Regulation of Basal Transcription." Gene, vol. 374, 2006, pp. 87-95.
Bolognese F, Pitarque-Martì M, Lo Cicero V, et al. Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription. Gene. 2006;374:87-95.
Bolognese, F., Pitarque-Martì, M., Lo Cicero, V., Mantovani, R., & Maier, J. A. (2006). Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription. Gene, 374, 87-95.
Bolognese F, et al. Characterization of the Human EDF-1 Minimal Promoter: Involvement of NFY and Sp1 in the Regulation of Basal Transcription. Gene. 2006 Jun 7;374:87-95. PubMed PMID: 16567061.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the human EDF-1 minimal promoter: involvement of NFY and Sp1 in the regulation of basal transcription. AU - Bolognese,Fabrizio, AU - Pitarque-Martì,Marià, AU - Lo Cicero,Viviana, AU - Mantovani,Roberto, AU - Maier,Jeanette A M, Y1 - 2006/03/29/ PY - 2005/04/06/received PY - 2006/01/24/revised PY - 2006/01/25/accepted PY - 2006/3/29/pubmed PY - 2006/7/26/medline PY - 2006/3/29/entrez SP - 87 EP - 95 JF - Gene JO - Gene VL - 374 N2 - Endothelial Differentiation Factor (EDF)-1 is a calmodulin binding protein involved in the repression of endothelial cell differentiation, a crucial, late step in angiogenesis. Its expression is cell cycle regulated, although its transcriptional regulation is yet to be determined. To map the promoter region and to understand its regulation, we cloned and fused 2300 bp upstream of EDF-1 translational start site to a luciferase reporter gene. After transient transfection in HeLa cells, this fragment was shown to possess a promoter activity. Deletion constructs of the 5' flanking region of EDF-1 lead to the identification of the minimal promoter region which was highly homologous to the mouse sequence. No TATA box was detected, whereas three consensus sequences--two GC boxes and a CAAT box--were identified. EMSA supershift and chromatin immunoprecipitation demonstrated that these sequences were binding sites for Sp1/Sp3 and NFY, respectively. Deletion of Sp1/Sp3 and NF-Y consensus sequences resulted in the total loss of EDF-1 promoter activity. Our studies indicate that Sp1 and NFY binding is essential for EDF-1 promoter activity. SN - 0378-1119 UR - https://www.unboundmedicine.com/medline/citation/16567061/Characterization_of_the_human_EDF_1_minimal_promoter:_involvement_of_NFY_and_Sp1_in_the_regulation_of_basal_transcription_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1119(06)00088-6 DB - PRIME DP - Unbound Medicine ER -