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Validated LC-MS-MS method for determination of m-nisoldipine polymorphs in rat plasma and its application to pharmacokinetic studies.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 01; 835(1-2):71-6.JC

Abstract

A sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS-MS) method has been developed to determine m-nisoldipine in rat plasma. Sample was pretreated by a single-step protein precipitation with acetonitrile, in contrast to the liquid-liquid procedure frequently used for the extraction of 1,4-dihydropyridines from biologic samples. Separation of analyte and internal standard (I.S.) was performed on a Symmetry RP-C(18) analytic column (50 mm x 4.6 mm, 3.5 microm) with a mobile phase consisting of acetonitrile-water (80:20, v/v) at a flow rate of 0.5 ml/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring (MRM) scan mode using TurboIonSpray ionization (ESI) source. The method was sensitive with a lower limit of quantification (LLOQ) of 0.2 ng/mL, with good linearity (r>or=0.9982) over the linear range of 0.2-20 ng/mL. All the validation data, such as accuracy, precision, and inter-day repeatability, were within the required limits. The method was successfully applied to pharmacokinetic and relative bioavailability studies of m-nisoldipine polymorphs in rats.

Authors+Show Affiliations

Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16567136

Citation

Wang, Hairong, et al. "Validated LC-MS-MS Method for Determination of M-nisoldipine Polymorphs in Rat Plasma and Its Application to Pharmacokinetic Studies." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 835, no. 1-2, 2006, pp. 71-6.
Wang H, Zhang L, Wang Q, et al. Validated LC-MS-MS method for determination of m-nisoldipine polymorphs in rat plasma and its application to pharmacokinetic studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2006;835(1-2):71-6.
Wang, H., Zhang, L., Wang, Q., Yuan, Z., & Li, M. (2006). Validated LC-MS-MS method for determination of m-nisoldipine polymorphs in rat plasma and its application to pharmacokinetic studies. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 835(1-2), 71-6.
Wang H, et al. Validated LC-MS-MS Method for Determination of M-nisoldipine Polymorphs in Rat Plasma and Its Application to Pharmacokinetic Studies. J Chromatogr B Analyt Technol Biomed Life Sci. 2006 May 1;835(1-2):71-6. PubMed PMID: 16567136.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validated LC-MS-MS method for determination of m-nisoldipine polymorphs in rat plasma and its application to pharmacokinetic studies. AU - Wang,Hairong, AU - Zhang,Lantong, AU - Wang,Qiao, AU - Yuan,Zhifang, AU - Li,Min, Y1 - 2006/03/29/ PY - 2005/11/30/received PY - 2006/01/27/revised PY - 2006/03/05/accepted PY - 2006/3/29/pubmed PY - 2006/6/20/medline PY - 2006/3/29/entrez SP - 71 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 835 IS - 1-2 N2 - A sensitive and specific liquid chromatography-tandem mass spectrometric (LC-MS-MS) method has been developed to determine m-nisoldipine in rat plasma. Sample was pretreated by a single-step protein precipitation with acetonitrile, in contrast to the liquid-liquid procedure frequently used for the extraction of 1,4-dihydropyridines from biologic samples. Separation of analyte and internal standard (I.S.) was performed on a Symmetry RP-C(18) analytic column (50 mm x 4.6 mm, 3.5 microm) with a mobile phase consisting of acetonitrile-water (80:20, v/v) at a flow rate of 0.5 ml/min. The API 4000 triple quadrupole mass spectrometer was operated in multiple reaction monitoring (MRM) scan mode using TurboIonSpray ionization (ESI) source. The method was sensitive with a lower limit of quantification (LLOQ) of 0.2 ng/mL, with good linearity (r>or=0.9982) over the linear range of 0.2-20 ng/mL. All the validation data, such as accuracy, precision, and inter-day repeatability, were within the required limits. The method was successfully applied to pharmacokinetic and relative bioavailability studies of m-nisoldipine polymorphs in rats. SN - 1570-0232 UR - https://www.unboundmedicine.com/medline/citation/16567136/Validated_LC_MS_MS_method_for_determination_of_m_nisoldipine_polymorphs_in_rat_plasma_and_its_application_to_pharmacokinetic_studies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(06)00228-5 DB - PRIME DP - Unbound Medicine ER -