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Separation and detection of peroxynitrite and its metabolites by capillary electrophoresis with UV detection.
J Chromatogr A. 2006 Apr 14; 1111(2):147-52.JC

Abstract

A method for the separation and direct detection of peroxynitrite (ONOO(-)) and two of its degradation products, nitrite (NO(2)(-)) and nitrate (NO(3)(-)), using capillary electrophoresis with ultraviolet detection is described. The separation parameters were optimized and included electrokinetic injection, a run buffer consisting of 25 mM K(2)HPO(4) 7.5 mM DTAB, pH 12, and a field strength of -323 V/cm. A diode array UV detector was employed in these studies as it allowed the determination of all three species simultaneously. Nitrate and nitrite provided the maximum response at 214 nm while peroxynitrite generated the best response at 302 nm. All three species could be detected at 214 nm, while simultaneous detection at 214 and 302 nm positively identified each peak.

Authors+Show Affiliations

Frankenfeld CN
Department of Pharmaceutical Chemistry, University of Kansas, Lawrence, 66047, USA.
Rosenbaugh MR
No affiliation info available
Fogarty BA
No affiliation info available
Lunte SM
No affiliation info available

MeSH

Electrophoresis, CapillaryPeroxynitrous AcidSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16569573

Citation

Frankenfeld, Celeste N., et al. "Separation and Detection of Peroxynitrite and Its Metabolites By Capillary Electrophoresis With UV Detection." Journal of Chromatography. A, vol. 1111, no. 2, 2006, pp. 147-52.
Frankenfeld CN, Rosenbaugh MR, Fogarty BA, et al. Separation and detection of peroxynitrite and its metabolites by capillary electrophoresis with UV detection. J Chromatogr A. 2006;1111(2):147-52.
Frankenfeld, C. N., Rosenbaugh, M. R., Fogarty, B. A., & Lunte, S. M. (2006). Separation and detection of peroxynitrite and its metabolites by capillary electrophoresis with UV detection. Journal of Chromatography. A, 1111(2), 147-52.
Frankenfeld CN, et al. Separation and Detection of Peroxynitrite and Its Metabolites By Capillary Electrophoresis With UV Detection. J Chromatogr A. 2006 Apr 14;1111(2):147-52. PubMed PMID: 16569573.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Separation and detection of peroxynitrite and its metabolites by capillary electrophoresis with UV detection. AU - Frankenfeld,Celeste N, AU - Rosenbaugh,Matt R, AU - Fogarty,Barbara A, AU - Lunte,Susan M, Y1 - 2005/06/13/ PY - 2005/03/01/received PY - 2005/04/24/revised PY - 2005/05/03/accepted PY - 2006/3/30/pubmed PY - 2006/5/31/medline PY - 2006/3/30/entrez SP - 147 EP - 52 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1111 IS - 2 N2 - A method for the separation and direct detection of peroxynitrite (ONOO(-)) and two of its degradation products, nitrite (NO(2)(-)) and nitrate (NO(3)(-)), using capillary electrophoresis with ultraviolet detection is described. The separation parameters were optimized and included electrokinetic injection, a run buffer consisting of 25 mM K(2)HPO(4) 7.5 mM DTAB, pH 12, and a field strength of -323 V/cm. A diode array UV detector was employed in these studies as it allowed the determination of all three species simultaneously. Nitrate and nitrite provided the maximum response at 214 nm while peroxynitrite generated the best response at 302 nm. All three species could be detected at 214 nm, while simultaneous detection at 214 and 302 nm positively identified each peak. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16569573/Separation_and_detection_of_peroxynitrite_and_its_metabolites_by_capillary_electrophoresis_with_UV_detection_ DB - PRIME DP - Unbound Medicine ER -