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Evaluation of human IgG subclass antibodies in the serodiagnosis of Paragonimiasis heterotremus.
Asian Pac J Allergy Immunol. 2005 Dec; 23(4):205-11.AP

Abstract

Immunoglobulin G subclass antibodies (IgG1, IgG2, IgG3, and IgG4) responses to the excretory-secretory antigens of the lung fluke, Paragonimus heterotremus, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for serodiagnosis of human paragonimiasis. Serum samples from patients with proven paragonimiasis, from patients with other parasitic infections, pulmonary tuberculosis and from healthy counterparts were analyzed. The results indicate that immunoblotting for the detection of IgG4 antibodies to an excretory-secretory product of P. heterotremus of an approximate molecular mass of 31.5 kDa, is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 97.6%, 100%, 96.9%, 90% and 100%, respectively.

Authors+Show Affiliations

Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16572740

Citation

Wongkham, Chaisiri, et al. "Evaluation of Human IgG Subclass Antibodies in the Serodiagnosis of Paragonimiasis Heterotremus." Asian Pacific Journal of Allergy and Immunology, vol. 23, no. 4, 2005, pp. 205-11.
Wongkham C, Intapan PM, Maleewong W, et al. Evaluation of human IgG subclass antibodies in the serodiagnosis of Paragonimiasis heterotremus. Asian Pac J Allergy Immunol. 2005;23(4):205-11.
Wongkham, C., Intapan, P. M., Maleewong, W., & Miwa, M. (2005). Evaluation of human IgG subclass antibodies in the serodiagnosis of Paragonimiasis heterotremus. Asian Pacific Journal of Allergy and Immunology, 23(4), 205-11.
Wongkham C, et al. Evaluation of Human IgG Subclass Antibodies in the Serodiagnosis of Paragonimiasis Heterotremus. Asian Pac J Allergy Immunol. 2005;23(4):205-11. PubMed PMID: 16572740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of human IgG subclass antibodies in the serodiagnosis of Paragonimiasis heterotremus. AU - Wongkham,Chaisiri, AU - Intapan,Pewpan M, AU - Maleewong,Wanchai, AU - Miwa,Masanao, PY - 2006/4/1/pubmed PY - 2006/9/2/medline PY - 2006/4/1/entrez SP - 205 EP - 11 JF - Asian Pacific journal of allergy and immunology JO - Asian Pac. J. Allergy Immunol. VL - 23 IS - 4 N2 - Immunoglobulin G subclass antibodies (IgG1, IgG2, IgG3, and IgG4) responses to the excretory-secretory antigens of the lung fluke, Paragonimus heterotremus, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for serodiagnosis of human paragonimiasis. Serum samples from patients with proven paragonimiasis, from patients with other parasitic infections, pulmonary tuberculosis and from healthy counterparts were analyzed. The results indicate that immunoblotting for the detection of IgG4 antibodies to an excretory-secretory product of P. heterotremus of an approximate molecular mass of 31.5 kDa, is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 97.6%, 100%, 96.9%, 90% and 100%, respectively. SN - 0125-877X UR - https://www.unboundmedicine.com/medline/citation/16572740/Evaluation_of_human_IgG_subclass_antibodies_in_the_serodiagnosis_of_Paragonimiasis_heterotremus_ L2 - http://apjai-journal.org/wp-content/uploads/2018/01/6EvaluationofHumanIgGSubclassVol23No4December2005P205.pdf DB - PRIME DP - Unbound Medicine ER -