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[Studies on chemical constituents of hairy root of Cassia obtusifolia].
Zhongguo Zhong Yao Za Zhi. 2006 Feb; 31(3):217-9.ZZ

Abstract

OBJECTIVE

To study the chemical constituents in hairy roots of Cassia obtusifolia.

METHOD

The hairy roots of C. obtusifolia were induced with Agrobacterium rhizogenes LBA9402 from cotyledons and cultured in MSO liquid medium. The compounds were isolated by silica gel, polyamide and Sephadex LH-20 column chromatography, and the structures were elucidated by employing chemical and spectral methods

RESULT

Eight compounds were isolated from the ethyl acetate fraction of 95% EtOH extract of the transformed roots of C. obtusifolia. They are betulinic acid, chrysophanol, physcion, stigmasterol, 1-hydroxy-7-methoxy-3-methyl-anthraquinone, 8-O-methylchrysophanol, 1-O-methylchrysophanol and aloe-emodin, and aloe-emodin was isolated from the hairy roots of C. obtusifolia for the first time.

CONCLUSION

The hairy roots of C. obtusifolia have the ability to synthesize the similar chemical constituents as the original plants.

Authors+Show Affiliations

College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

chi

PubMed ID

16573002

Citation

Yang, Shi-Hai, et al. "[Studies On Chemical Constituents of Hairy Root of Cassia Obtusifolia]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 31, no. 3, 2006, pp. 217-9.
Yang SH, Guo HZ, Guo DA, et al. [Studies on chemical constituents of hairy root of Cassia obtusifolia]. Zhongguo Zhong Yao Za Zhi. 2006;31(3):217-9.
Yang, S. H., Guo, H. Z., Guo, D. A., & Zheng, J. H. (2006). [Studies on chemical constituents of hairy root of Cassia obtusifolia]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 31(3), 217-9.
Yang SH, et al. [Studies On Chemical Constituents of Hairy Root of Cassia Obtusifolia]. Zhongguo Zhong Yao Za Zhi. 2006;31(3):217-9. PubMed PMID: 16573002.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Studies on chemical constituents of hairy root of Cassia obtusifolia]. AU - Yang,Shi-Hai, AU - Guo,Hong-Zhu, AU - Guo,De-An, AU - Zheng,Jun-Hua, PY - 2006/4/1/pubmed PY - 2006/7/26/medline PY - 2006/4/1/entrez SP - 217 EP - 9 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 31 IS - 3 N2 - OBJECTIVE: To study the chemical constituents in hairy roots of Cassia obtusifolia. METHOD: The hairy roots of C. obtusifolia were induced with Agrobacterium rhizogenes LBA9402 from cotyledons and cultured in MSO liquid medium. The compounds were isolated by silica gel, polyamide and Sephadex LH-20 column chromatography, and the structures were elucidated by employing chemical and spectral methods RESULT: Eight compounds were isolated from the ethyl acetate fraction of 95% EtOH extract of the transformed roots of C. obtusifolia. They are betulinic acid, chrysophanol, physcion, stigmasterol, 1-hydroxy-7-methoxy-3-methyl-anthraquinone, 8-O-methylchrysophanol, 1-O-methylchrysophanol and aloe-emodin, and aloe-emodin was isolated from the hairy roots of C. obtusifolia for the first time. CONCLUSION: The hairy roots of C. obtusifolia have the ability to synthesize the similar chemical constituents as the original plants. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/16573002/[Studies_on_chemical_constituents_of_hairy_root_of_Cassia_obtusifolia]_ DB - PRIME DP - Unbound Medicine ER -