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Dynamic pathway allocation in early terpenoid biosynthesis of stress-induced lima bean leaves.
Phytochemistry. 2006 Aug; 67(15):1661-72.P

Abstract

Two independent pathways contribute in higher plants to the formation of isopenteny1 diphosphate (IDP), the central building block of isoprenoids. In general, the cytosolic mevalonate pathway (MVA) provides the precursors for sesquiterpenes and sterols, whereas the plastidial methylerythritol pathway (MEP) furnishes the monoterpene-, diterpene- and carotenoids. Administration of deuterium labeled 1-deoxy-d-xylulose and mevalolactone to lima beans (Phaseolus lunatus), followed by gas chromatographic separation and mass spectrometric analysis of de novo produced volatiles revealed that the strict separation of both pathways does not exist. This could be confirmed by blocking the pathways individually with cerivastatin((R)) (MVA) and fosmidomycin (MEP), respectively. Isotopic ratio mass spectrometry (IRMS) at natural abundance levels demonstrated independently and without the need for labeled precursors a dynamic allocation of the MVA- or the MEP-pathway in the biosynthesis of the nerolidol-derived homoterpene 4,8-dimethy1-nona-1,3,7-triene (DMNT). Insect-feeding upregulated predominantly the MVA-pathway, while the fungal elicitor alamethicin stimulated the biosynthesis of DMNT via the MEP-pathway.

Authors+Show Affiliations

Max Planck Institute for Chemical Ecology, Beutenbergcampus, Hans-Knöll-Strasse 8, D-07745 Jena, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16580034

Citation

Bartram, Stefan, et al. "Dynamic Pathway Allocation in Early Terpenoid Biosynthesis of Stress-induced Lima Bean Leaves." Phytochemistry, vol. 67, no. 15, 2006, pp. 1661-72.
Bartram S, Jux A, Gleixner G, et al. Dynamic pathway allocation in early terpenoid biosynthesis of stress-induced lima bean leaves. Phytochemistry. 2006;67(15):1661-72.
Bartram, S., Jux, A., Gleixner, G., & Boland, W. (2006). Dynamic pathway allocation in early terpenoid biosynthesis of stress-induced lima bean leaves. Phytochemistry, 67(15), 1661-72.
Bartram S, et al. Dynamic Pathway Allocation in Early Terpenoid Biosynthesis of Stress-induced Lima Bean Leaves. Phytochemistry. 2006;67(15):1661-72. PubMed PMID: 16580034.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dynamic pathway allocation in early terpenoid biosynthesis of stress-induced lima bean leaves. AU - Bartram,Stefan, AU - Jux,Andreas, AU - Gleixner,Gerd, AU - Boland,Wilhelm, Y1 - 2006/03/31/ PY - 2005/11/07/received PY - 2005/12/06/revised PY - 2006/4/4/pubmed PY - 2006/10/25/medline PY - 2006/4/4/entrez SP - 1661 EP - 72 JF - Phytochemistry JO - Phytochemistry VL - 67 IS - 15 N2 - Two independent pathways contribute in higher plants to the formation of isopenteny1 diphosphate (IDP), the central building block of isoprenoids. In general, the cytosolic mevalonate pathway (MVA) provides the precursors for sesquiterpenes and sterols, whereas the plastidial methylerythritol pathway (MEP) furnishes the monoterpene-, diterpene- and carotenoids. Administration of deuterium labeled 1-deoxy-d-xylulose and mevalolactone to lima beans (Phaseolus lunatus), followed by gas chromatographic separation and mass spectrometric analysis of de novo produced volatiles revealed that the strict separation of both pathways does not exist. This could be confirmed by blocking the pathways individually with cerivastatin((R)) (MVA) and fosmidomycin (MEP), respectively. Isotopic ratio mass spectrometry (IRMS) at natural abundance levels demonstrated independently and without the need for labeled precursors a dynamic allocation of the MVA- or the MEP-pathway in the biosynthesis of the nerolidol-derived homoterpene 4,8-dimethy1-nona-1,3,7-triene (DMNT). Insect-feeding upregulated predominantly the MVA-pathway, while the fungal elicitor alamethicin stimulated the biosynthesis of DMNT via the MEP-pathway. SN - 0031-9422 UR - https://www.unboundmedicine.com/medline/citation/16580034/Dynamic_pathway_allocation_in_early_terpenoid_biosynthesis_of_stress_induced_lima_bean_leaves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0031-9422(06)00100-2 DB - PRIME DP - Unbound Medicine ER -