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Determination of hesperidin in Pericarpium Citri Reticulatae by semi-micro HPLC with electrochemical detection.
J Pharm Biomed Anal. 2006 Jun 16; 41(4):1401-5.JP

Abstract

Determination of hesperidin contents in Pericarpium Citri Reticulatae was performed by a simple extraction with methanol and semi-micro high-performance liquid chromatography with electrochemical detection (muHPLC-ECD). Chromatography was performed using a microbore octadecylsilica (ODS) column, methanol-water-phosphoric acid (40:60:0.5, v/v/v), as a mobile phase and applied potential at +0.9 V versus Ag/AgCl. Peak heights were found linearly related to the concentrations of hesperidin injected 9.16 ng/ml to 3.06 microg/ml (r>0.999). The detection limit (S/N=3) was 3.06 ng/ml (15.3 pg). Hesperidin of 305 ng/ml was detected with a relative standard deviation (R.S.D.) of 0.79% (n=5). Hesperidin in Pericarpium Citri Reticulatae was extracted with methanol, diluted with the mobile phase, and injected into the muHPLC-ECD for determination. The hesperidin content of Pericarpium Citri Reticulatae from four different districts in China were determined with R.S.D. of 3.59%, 2.29%, 2.36%, and 2.32% (n=5), respectively. Recoveries of hesperidin from the four Pericarpium Citri Reticulatae sources were 100.3%, 99.83%, 100.7%, and 100.6%, respectively. This method is useful for the determination of hesperidin in Pericarpium Citri Reticulatae, and especially so for instances when samples are sparse.

Authors+Show Affiliations

Department of Traditional Chinese Medicine, Shanghai Institute for Drug Control, No 615 Liuzhou Road, Shanghai 200233, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16580168

Citation

Xia, Jing, et al. "Determination of Hesperidin in Pericarpium Citri Reticulatae By Semi-micro HPLC With Electrochemical Detection." Journal of Pharmaceutical and Biomedical Analysis, vol. 41, no. 4, 2006, pp. 1401-5.
Xia J, Kotani A, Hakamata H, et al. Determination of hesperidin in Pericarpium Citri Reticulatae by semi-micro HPLC with electrochemical detection. J Pharm Biomed Anal. 2006;41(4):1401-5.
Xia, J., Kotani, A., Hakamata, H., & Kusu, F. (2006). Determination of hesperidin in Pericarpium Citri Reticulatae by semi-micro HPLC with electrochemical detection. Journal of Pharmaceutical and Biomedical Analysis, 41(4), 1401-5.
Xia J, et al. Determination of Hesperidin in Pericarpium Citri Reticulatae By Semi-micro HPLC With Electrochemical Detection. J Pharm Biomed Anal. 2006 Jun 16;41(4):1401-5. PubMed PMID: 16580168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of hesperidin in Pericarpium Citri Reticulatae by semi-micro HPLC with electrochemical detection. AU - Xia,Jing, AU - Kotani,Akira, AU - Hakamata,Hideki, AU - Kusu,Fumiyo, Y1 - 2006/03/31/ PY - 2005/12/27/received PY - 2006/02/15/revised PY - 2006/02/21/accepted PY - 2006/4/4/pubmed PY - 2006/8/3/medline PY - 2006/4/4/entrez SP - 1401 EP - 5 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 41 IS - 4 N2 - Determination of hesperidin contents in Pericarpium Citri Reticulatae was performed by a simple extraction with methanol and semi-micro high-performance liquid chromatography with electrochemical detection (muHPLC-ECD). Chromatography was performed using a microbore octadecylsilica (ODS) column, methanol-water-phosphoric acid (40:60:0.5, v/v/v), as a mobile phase and applied potential at +0.9 V versus Ag/AgCl. Peak heights were found linearly related to the concentrations of hesperidin injected 9.16 ng/ml to 3.06 microg/ml (r>0.999). The detection limit (S/N=3) was 3.06 ng/ml (15.3 pg). Hesperidin of 305 ng/ml was detected with a relative standard deviation (R.S.D.) of 0.79% (n=5). Hesperidin in Pericarpium Citri Reticulatae was extracted with methanol, diluted with the mobile phase, and injected into the muHPLC-ECD for determination. The hesperidin content of Pericarpium Citri Reticulatae from four different districts in China were determined with R.S.D. of 3.59%, 2.29%, 2.36%, and 2.32% (n=5), respectively. Recoveries of hesperidin from the four Pericarpium Citri Reticulatae sources were 100.3%, 99.83%, 100.7%, and 100.6%, respectively. This method is useful for the determination of hesperidin in Pericarpium Citri Reticulatae, and especially so for instances when samples are sparse. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/16580168/Determination_of_hesperidin_in_Pericarpium_Citri_Reticulatae_by_semi_micro_HPLC_with_electrochemical_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(06)00212-3 DB - PRIME DP - Unbound Medicine ER -