Tags

Type your tag names separated by a space and hit enter

Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic ovary syndrome.
Fertil Steril. 2006 Apr; 85(4):1017-26.FS

Abstract

OBJECTIVE

To evaluate gene and protein expression of steroid receptors, nuclear receptor coregulators, and uterine receptivity markers in midsecretory phase endometria from untreated women with polycystic ovary syndrome (PCOS).

DESIGN

Case-control study.

SETTING

Hospital research unit.

PATIENT(S)

Eight patients with PCOS and eight fertile women of similar age to those with PCOS.

INTERVENTION(S)

Endometrial samples were obtained from women with PCOS (PCOSE) and normal (NE) women during the midsecretory phase of the menstrual cycle.

MAIN OUTCOME MEASURE(S)

Expression studies (immunohistochemistry, reverse transcription-polymerase chain reaction [RT-PCR] and Western blot).

RESULT(S)

Endometria from PCOS exhibit higher levels of messenger RNA (mRNA) and protein for estrogen receptor alpha and coactivators than NE. Epithelial cells had a greater expression of progesterone receptor in PCOSE, whereas, no differences were observed in gene and protein expression of the nuclear corepressor (NcoR) and the antiadhesion molecule mucin type-1 (MUC-1) between PCOSE and NE. Immunodetection for the coactivator ARA70 was higher in PCOSE than in NE; in contrast, expression of beta3-integrin in epithelia was lower in PCOSE than in control endometria.

CONCLUSION(S)

The higher response to steroid hormones of endometria from untreated PCOS-women induces diminished expression of beta3 integrin, which partially explain implantation failure in PCOS patients.

Authors+Show Affiliations

Institute of Maternal and Child Research, School of Medicine, San Borja-Arriarán Clinical Hospital, University of Chile, Santiago, Chile.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16580389

Citation

Quezada, Susana, et al. "Evaluation of Steroid Receptors, Coregulators, and Molecules Associated With Uterine Receptivity in Secretory Endometria From Untreated Women With Polycystic Ovary Syndrome." Fertility and Sterility, vol. 85, no. 4, 2006, pp. 1017-26.
Quezada S, Avellaira C, Johnson MC, et al. Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic ovary syndrome. Fertil Steril. 2006;85(4):1017-26.
Quezada, S., Avellaira, C., Johnson, M. C., Gabler, F., Fuentes, A., & Vega, M. (2006). Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic ovary syndrome. Fertility and Sterility, 85(4), 1017-26.
Quezada S, et al. Evaluation of Steroid Receptors, Coregulators, and Molecules Associated With Uterine Receptivity in Secretory Endometria From Untreated Women With Polycystic Ovary Syndrome. Fertil Steril. 2006;85(4):1017-26. PubMed PMID: 16580389.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of steroid receptors, coregulators, and molecules associated with uterine receptivity in secretory endometria from untreated women with polycystic ovary syndrome. AU - Quezada,Susana, AU - Avellaira,Carla, AU - Johnson,Maria Cecilia, AU - Gabler,Fernando, AU - Fuentes,Ariel, AU - Vega,Margarita, Y1 - 2006/03/09/ PY - 2005/04/14/received PY - 2005/09/16/revised PY - 2005/09/16/accepted PY - 2006/4/4/pubmed PY - 2006/5/12/medline PY - 2006/4/4/entrez SP - 1017 EP - 26 JF - Fertility and sterility JO - Fertil. Steril. VL - 85 IS - 4 N2 - OBJECTIVE: To evaluate gene and protein expression of steroid receptors, nuclear receptor coregulators, and uterine receptivity markers in midsecretory phase endometria from untreated women with polycystic ovary syndrome (PCOS). DESIGN: Case-control study. SETTING: Hospital research unit. PATIENT(S): Eight patients with PCOS and eight fertile women of similar age to those with PCOS. INTERVENTION(S): Endometrial samples were obtained from women with PCOS (PCOSE) and normal (NE) women during the midsecretory phase of the menstrual cycle. MAIN OUTCOME MEASURE(S): Expression studies (immunohistochemistry, reverse transcription-polymerase chain reaction [RT-PCR] and Western blot). RESULT(S): Endometria from PCOS exhibit higher levels of messenger RNA (mRNA) and protein for estrogen receptor alpha and coactivators than NE. Epithelial cells had a greater expression of progesterone receptor in PCOSE, whereas, no differences were observed in gene and protein expression of the nuclear corepressor (NcoR) and the antiadhesion molecule mucin type-1 (MUC-1) between PCOSE and NE. Immunodetection for the coactivator ARA70 was higher in PCOSE than in NE; in contrast, expression of beta3-integrin in epithelia was lower in PCOSE than in control endometria. CONCLUSION(S): The higher response to steroid hormones of endometria from untreated PCOS-women induces diminished expression of beta3 integrin, which partially explain implantation failure in PCOS patients. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/16580389/Evaluation_of_steroid_receptors_coregulators_and_molecules_associated_with_uterine_receptivity_in_secretory_endometria_from_untreated_women_with_polycystic_ovary_syndrome_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(06)00002-1 DB - PRIME DP - Unbound Medicine ER -