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Morphological and binding properties of interleukin-6 on thin ZnO films grown on (100) silicon substrates for biosensor applications.
Biosens Bioelectron. 2006 Dec 15; 22(5):707-14.BB

Abstract

To develop effective protein immobilization technology with minimal amounts of protein for high sensitivity surface acoustic wave biosensors, we determined the binding properties, and morphological characteristics of human interleukin-6 (IL-6), a pro-inflammatory cytokine, on the surface of ZnO, and SiO(2) films grown onto (100) Si substrates, for the first time. Interleukin-6 was immobilized in the range of 0.276-10 pg/ml on the surface of ZnO and SiO(2), and visualized at each stage, while protein-protein interactions were measured with the antigen/antibody immunoassay of solid-phase ELISA, which we modified for these types of substrates. A relative mass value was determined in each case. ELISA detected upward of 1 and 6 ng/ml of protein applied on ZnO and SiO(2), respectively. It is concluded that the more reactive ZnO surface is a new and more effective template for protein immobilization.

Authors+Show Affiliations

Krishnamoorthy S
Department of Electrical and Computer Engineering, University of Maryland, College Park, MD 20742, USA.
Bei T
No affiliation info available
Zoumakis E
No affiliation info available
Chrousos GP
No affiliation info available
Iliadis AA
No affiliation info available

MeSH

Binding SitesBiosensing TechniquesCoated Materials, BiocompatibleCrystallizationImmunoassayInterleukin-6Materials TestingProtein BindingSerum Albumin, BovineSiliconSurface PropertiesZinc Oxide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

16581242

Citation

Krishnamoorthy, Soumya, et al. "Morphological and Binding Properties of Interleukin-6 On Thin ZnO Films Grown On (100) Silicon Substrates for Biosensor Applications." Biosensors & Bioelectronics, vol. 22, no. 5, 2006, pp. 707-14.
Krishnamoorthy S, Bei T, Zoumakis E, et al. Morphological and binding properties of interleukin-6 on thin ZnO films grown on (100) silicon substrates for biosensor applications. Biosens Bioelectron. 2006;22(5):707-14.
Krishnamoorthy, S., Bei, T., Zoumakis, E., Chrousos, G. P., & Iliadis, A. A. (2006). Morphological and binding properties of interleukin-6 on thin ZnO films grown on (100) silicon substrates for biosensor applications. Biosensors & Bioelectronics, 22(5), 707-14.
Krishnamoorthy S, et al. Morphological and Binding Properties of Interleukin-6 On Thin ZnO Films Grown On (100) Silicon Substrates for Biosensor Applications. Biosens Bioelectron. 2006 Dec 15;22(5):707-14. PubMed PMID: 16581242.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Morphological and binding properties of interleukin-6 on thin ZnO films grown on (100) silicon substrates for biosensor applications. AU - Krishnamoorthy,Soumya, AU - Bei,Thaleia, AU - Zoumakis,Emmanouil, AU - Chrousos,George P, AU - Iliadis,Agis A, Y1 - 2006/04/11/ PY - 2005/11/22/received PY - 2006/01/27/revised PY - 2006/02/14/accepted PY - 2006/4/4/pubmed PY - 2007/1/18/medline PY - 2006/4/4/entrez SP - 707 EP - 14 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 22 IS - 5 N2 - To develop effective protein immobilization technology with minimal amounts of protein for high sensitivity surface acoustic wave biosensors, we determined the binding properties, and morphological characteristics of human interleukin-6 (IL-6), a pro-inflammatory cytokine, on the surface of ZnO, and SiO(2) films grown onto (100) Si substrates, for the first time. Interleukin-6 was immobilized in the range of 0.276-10 pg/ml on the surface of ZnO and SiO(2), and visualized at each stage, while protein-protein interactions were measured with the antigen/antibody immunoassay of solid-phase ELISA, which we modified for these types of substrates. A relative mass value was determined in each case. ELISA detected upward of 1 and 6 ng/ml of protein applied on ZnO and SiO(2), respectively. It is concluded that the more reactive ZnO surface is a new and more effective template for protein immobilization. SN - 0956-5663 UR - https://www.unboundmedicine.com/medline/citation/16581242/Morphological_and_binding_properties_of_interleukin_6_on_thin_ZnO_films_grown_on__100__silicon_substrates_for_biosensor_applications_ DB - PRIME DP - Unbound Medicine ER -