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Determination of cocaine and cocaethylene in urine by solid-phase microextraction and gas chromatography-mass spectrometry.
Biomed Chromatogr. 2006 Oct; 20(10):1071-5.BC

Abstract

In order to evaluate recent cocaine exposure or its coingestion with ethanol, a simple and sensitive solid-phase microextraction (SPME) procedure for determination of cocaine and cocaethylene in urine was developed and validated. A polydimethylsiloxane fibre (100 microm) was submersed in the urine sample for 20 min under magnetic stirring after alkalinization with solid buffer (NaHCO(3):K(2)CO(3), 2:1). Gas chromatography-mass spectrometry (GC-MS) was used to identify and quantify the analytes in selected ion monitoring mode (SIM). The limits of quantification were 5.0 ng/mL for both analytes. Good inter- and intra-assay precision was also observed (coefficient of variation <9%).

Authors+Show Affiliations

Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Av. Professor Lineu Prestes, 580 B13B, CEP 05508-900, São Paulo, SP, Brazil. yonamine@usp.brNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

16583460

Citation

Yonamine, Mauricio, and Alessandro Morais Saviano. "Determination of Cocaine and Cocaethylene in Urine By Solid-phase Microextraction and Gas Chromatography-mass Spectrometry." Biomedical Chromatography : BMC, vol. 20, no. 10, 2006, pp. 1071-5.
Yonamine M, Saviano AM. Determination of cocaine and cocaethylene in urine by solid-phase microextraction and gas chromatography-mass spectrometry. Biomed Chromatogr. 2006;20(10):1071-5.
Yonamine, M., & Saviano, A. M. (2006). Determination of cocaine and cocaethylene in urine by solid-phase microextraction and gas chromatography-mass spectrometry. Biomedical Chromatography : BMC, 20(10), 1071-5.
Yonamine M, Saviano AM. Determination of Cocaine and Cocaethylene in Urine By Solid-phase Microextraction and Gas Chromatography-mass Spectrometry. Biomed Chromatogr. 2006;20(10):1071-5. PubMed PMID: 16583460.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of cocaine and cocaethylene in urine by solid-phase microextraction and gas chromatography-mass spectrometry. AU - Yonamine,Mauricio, AU - Saviano,Alessandro Morais, PY - 2006/4/4/pubmed PY - 2007/1/24/medline PY - 2006/4/4/entrez SP - 1071 EP - 5 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 20 IS - 10 N2 - In order to evaluate recent cocaine exposure or its coingestion with ethanol, a simple and sensitive solid-phase microextraction (SPME) procedure for determination of cocaine and cocaethylene in urine was developed and validated. A polydimethylsiloxane fibre (100 microm) was submersed in the urine sample for 20 min under magnetic stirring after alkalinization with solid buffer (NaHCO(3):K(2)CO(3), 2:1). Gas chromatography-mass spectrometry (GC-MS) was used to identify and quantify the analytes in selected ion monitoring mode (SIM). The limits of quantification were 5.0 ng/mL for both analytes. Good inter- and intra-assay precision was also observed (coefficient of variation <9%). SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/16583460/Determination_of_cocaine_and_cocaethylene_in_urine_by_solid_phase_microextraction_and_gas_chromatography_mass_spectrometry_ L2 - https://doi.org/10.1002/bmc.643 DB - PRIME DP - Unbound Medicine ER -