High-performance liquid chromatographic identification of flavonoid monoglycosides from Prunus serotina ehrh.Acta Pol Pharm. 2005 Nov-Dec; 62(6):435-41.AP
Five minor flavonoid monosides, glycosides of quercetin and kaempferol, together with three previously isolated compounds were identified cochromatographically in P. serotina Ehrh. leaves and flowers (inflorescences) using RP-HPLC and TLC techniques and finally determined as quercetin 3-O-alpha-L-arabinofuranoside (avicularin), 3-O-alpha-L-arabinopyranoside (guaijaverin), 3-O-beta-D-xylopyranoside (reynoutrin), 3-O-beta-D-glucopyranoside (isoquercitrin), 3-O-beta-D-galactopyranoside (hyperoside) followed by kaempferol 3-O-alpha-L-arabinofuranoside (juglanin), 3-O-beta-D-xylopyranoside and 3-O-beta-D-glucopyranoside (astragalin). Moreover, two further minor flavonols were isolated from the leaves, characterized by hydrolysis experiments, UV and 1H NMR spectroscopy, and identified finally as isorhamnetin 3-O-alpha-arabinofuranoside and isorhamnetin 3-O-beta-xylopyranoside, the rare natural products.