Tags

Type your tag names separated by a space and hit enter

Diagnostic value of specific local antibody production and nucleic acid amplification technique-nested polymerase chain reaction (nPCR) in clinically suspected ocular toxoplasmosis.
Ocul Immunol Inflamm 2006; 14(2):105-12OI

Abstract

OBJECTIVE

The study was to evaluate the diagnostic efficacy of nested polymerase chain reaction (nPCR) using primers targeting B1 gene of Toxoplasma gondii (T. gondii) with Witmer Desmonts coefficient (WDC) technique in intraocular fluids of clinically suspected toxoplasma retino choroiditis (TRC) patients.

MATERIALS AND METHODS

Two hundred and seventy eight specimens from 189 patients (25 TRC patients and 164 controls) consisting of 189 serum samples and 89 intraocular fluids were included in the study. The clinical specimens were categorized into TRC patients (typical TRC lesion-group I & atypical TRC lesion-group II) and controls (voluntary blood donors-group III, patients undergoing uncomplicated cataract surgery-group IV, ocular inflammation of non-toxoplasma origin-group V). Detection of anti T. gondii IgG and IgM antibodies in serum samples and intraocular fluids were performed and WDC was calculated by the standard method. The standardized nPCR was applied on the 89 intraocular fluids.

RESULTS

Clinical diagnosis of TRC based on fundus examination was considered to be the "gold standard." Anti T. gondii IgG/IgM antibodies were detected in serum by ELISA in 95.6% of 25 clinically suspected TRC patients (gp I and II), 28% of gp III, 40.4% of gp IV, and in 58.3% of gpV. Witmer Desmont's coefficient was positive in 72.7% (16/22) and nPCR in 59.1% (13/22) of TRC patients (gp I and II). Both WDC and nPCR were negative in all the controls. The difference in sensitivity of WDC and nPCR was not statistically significant (p=0.5247).

CONCLUSIONS

Though both WDC and nPCR were reliable diagnostic techniques for ocular toxoplasmosis, nPCR is more acceptable because of the amount of specimen(s) required, rapidity, cost effectiveness, and direct evidence of T. gondii DNA in the intraocular fluids.

Authors+Show Affiliations

L&T Microbiology Research Centre, Vision Research Foundation, Sankara Nethralaya, Chennai, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

16597540

Citation

Mahalakshmi, B, et al. "Diagnostic Value of Specific Local Antibody Production and Nucleic Acid Amplification Technique-nested Polymerase Chain Reaction (nPCR) in Clinically Suspected Ocular Toxoplasmosis." Ocular Immunology and Inflammation, vol. 14, no. 2, 2006, pp. 105-12.
Mahalakshmi B, Therese KL, Madhavan HN, et al. Diagnostic value of specific local antibody production and nucleic acid amplification technique-nested polymerase chain reaction (nPCR) in clinically suspected ocular toxoplasmosis. Ocul Immunol Inflamm. 2006;14(2):105-12.
Mahalakshmi, B., Therese, K. L., Madhavan, H. N., & Biswas, J. (2006). Diagnostic value of specific local antibody production and nucleic acid amplification technique-nested polymerase chain reaction (nPCR) in clinically suspected ocular toxoplasmosis. Ocular Immunology and Inflammation, 14(2), pp. 105-12.
Mahalakshmi B, et al. Diagnostic Value of Specific Local Antibody Production and Nucleic Acid Amplification Technique-nested Polymerase Chain Reaction (nPCR) in Clinically Suspected Ocular Toxoplasmosis. Ocul Immunol Inflamm. 2006;14(2):105-12. PubMed PMID: 16597540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnostic value of specific local antibody production and nucleic acid amplification technique-nested polymerase chain reaction (nPCR) in clinically suspected ocular toxoplasmosis. AU - Mahalakshmi,B, AU - Therese,K Lily, AU - Madhavan,H N, AU - Biswas,J, PY - 2006/4/7/pubmed PY - 2006/6/30/medline PY - 2006/4/7/entrez SP - 105 EP - 12 JF - Ocular immunology and inflammation JO - Ocul. Immunol. Inflamm. VL - 14 IS - 2 N2 - OBJECTIVE: The study was to evaluate the diagnostic efficacy of nested polymerase chain reaction (nPCR) using primers targeting B1 gene of Toxoplasma gondii (T. gondii) with Witmer Desmonts coefficient (WDC) technique in intraocular fluids of clinically suspected toxoplasma retino choroiditis (TRC) patients. MATERIALS AND METHODS: Two hundred and seventy eight specimens from 189 patients (25 TRC patients and 164 controls) consisting of 189 serum samples and 89 intraocular fluids were included in the study. The clinical specimens were categorized into TRC patients (typical TRC lesion-group I & atypical TRC lesion-group II) and controls (voluntary blood donors-group III, patients undergoing uncomplicated cataract surgery-group IV, ocular inflammation of non-toxoplasma origin-group V). Detection of anti T. gondii IgG and IgM antibodies in serum samples and intraocular fluids were performed and WDC was calculated by the standard method. The standardized nPCR was applied on the 89 intraocular fluids. RESULTS: Clinical diagnosis of TRC based on fundus examination was considered to be the "gold standard." Anti T. gondii IgG/IgM antibodies were detected in serum by ELISA in 95.6% of 25 clinically suspected TRC patients (gp I and II), 28% of gp III, 40.4% of gp IV, and in 58.3% of gpV. Witmer Desmont's coefficient was positive in 72.7% (16/22) and nPCR in 59.1% (13/22) of TRC patients (gp I and II). Both WDC and nPCR were negative in all the controls. The difference in sensitivity of WDC and nPCR was not statistically significant (p=0.5247). CONCLUSIONS: Though both WDC and nPCR were reliable diagnostic techniques for ocular toxoplasmosis, nPCR is more acceptable because of the amount of specimen(s) required, rapidity, cost effectiveness, and direct evidence of T. gondii DNA in the intraocular fluids. SN - 0927-3948 UR - https://www.unboundmedicine.com/medline/citation/16597540/Diagnostic_value_of_specific_local_antibody_production_and_nucleic_acid_amplification_technique_nested_polymerase_chain_reaction__nPCR__in_clinically_suspected_ocular_toxoplasmosis_ L2 - http://www.tandfonline.com/doi/full/10.1080/09273940500545692 DB - PRIME DP - Unbound Medicine ER -