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Determination of valdecoxib in serum using a HPLC-diode array detector and its application in a pharmacokinetic study.
Pharmazie. 2006 Mar; 61(3):245-6.P

Abstract

A simple, sensitive, isocratic and reproducible reversed phase HPLC method for the determination of valdecoxib, a novel specific COX-2 inhibitor in human serum was developed using a diode array detector and celecoxib as internal standard. The system consisted of a C18 column and a detector set at 240 nm. The mobile phase was a mixture of acetonitrile:water acidified to pH 3.2 with orthophosphoric acid (OPA) (60:40) pumped at room temperature and a flow rate of 1 ml/min. The mean absolute recovery value was about 90%, while the intra (n = 5) and inter (n = 5) assay variations were <18%. The calibration was linear over a concentration range of 20 ng/ml to 200 microg/ml with r2 > 0.999. The limit of detection was < or = 10 ng/ml. The method was used to study the pharmacokinetics of valdecoxib after a single dose oral administration to human volunteers.

Authors+Show Affiliations

Faculty of Pharmaceutical Sciences, Kakatiya University, Warangal, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Journal Article

Language

eng

PubMed ID

16599270

Citation

Keshetty, S, et al. "Determination of Valdecoxib in Serum Using a HPLC-diode Array Detector and Its Application in a Pharmacokinetic Study." Die Pharmazie, vol. 61, no. 3, 2006, pp. 245-6.
Keshetty S, Venisetty RK, Molmoori V, et al. Determination of valdecoxib in serum using a HPLC-diode array detector and its application in a pharmacokinetic study. Pharmazie. 2006;61(3):245-6.
Keshetty, S., Venisetty, R. K., Molmoori, V., & Ciddi, V. (2006). Determination of valdecoxib in serum using a HPLC-diode array detector and its application in a pharmacokinetic study. Die Pharmazie, 61(3), 245-6.
Keshetty S, et al. Determination of Valdecoxib in Serum Using a HPLC-diode Array Detector and Its Application in a Pharmacokinetic Study. Pharmazie. 2006;61(3):245-6. PubMed PMID: 16599270.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of valdecoxib in serum using a HPLC-diode array detector and its application in a pharmacokinetic study. AU - Keshetty,S, AU - Venisetty,R K, AU - Molmoori,V, AU - Ciddi,V, PY - 2006/4/8/pubmed PY - 2006/5/18/medline PY - 2006/4/8/entrez SP - 245 EP - 6 JF - Die Pharmazie JO - Pharmazie VL - 61 IS - 3 N2 - A simple, sensitive, isocratic and reproducible reversed phase HPLC method for the determination of valdecoxib, a novel specific COX-2 inhibitor in human serum was developed using a diode array detector and celecoxib as internal standard. The system consisted of a C18 column and a detector set at 240 nm. The mobile phase was a mixture of acetonitrile:water acidified to pH 3.2 with orthophosphoric acid (OPA) (60:40) pumped at room temperature and a flow rate of 1 ml/min. The mean absolute recovery value was about 90%, while the intra (n = 5) and inter (n = 5) assay variations were <18%. The calibration was linear over a concentration range of 20 ng/ml to 200 microg/ml with r2 > 0.999. The limit of detection was < or = 10 ng/ml. The method was used to study the pharmacokinetics of valdecoxib after a single dose oral administration to human volunteers. SN - 0031-7144 UR - https://www.unboundmedicine.com/medline/citation/16599270/Determination_of_valdecoxib_in_serum_using_a_HPLC_diode_array_detector_and_its_application_in_a_pharmacokinetic_study_ L2 - https://antibodies.cancer.gov/detail/CPTC-RRM2-2 DB - PRIME DP - Unbound Medicine ER -