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DNA binding by the archaeal histone HMf results in positive supercoiling.
Proc Natl Acad Sci U S A. 1991 Dec 01; 88(23):10397-401.PN

Abstract

HMf, a histone from the hyperthermophilic archaeon Methanothermus fervidus binds double-stranded DNA molecules in vitro, forming compact structures that visibly resemble eukaryal nucleosomes. We show here that HMf binding increases the helical periodicity of DNA molecules to approximately 11 base pairs (bp) per turn and that DNA molecules in these nucleosome-like structures are constrained in positive toroidal supercoils. Based on the mass of HMf needed to cause a change in linking number (delta Lk), the maximum delta Lk introduced into circular DNA molecules of known sizes, and electron microscopy, we estimate that each HMf-DNA structure contains between 90 and 150 bp of DNA wrapped in 1.5 positive toroidal supercoils around a core of four HMf molecules. A model and pathway for the formation of these structures in vitro are presented and the possible role of positive toroidal wrapping of the M. fervidus genome in vivo is discussed.

Authors+Show Affiliations

Department of Microbiology, Ohio State University, Columbus 43210.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

1660135

Citation

Musgrave, D R., et al. "DNA Binding By the Archaeal Histone HMf Results in Positive Supercoiling." Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 23, 1991, pp. 10397-401.
Musgrave DR, Sandman KM, Reeve JN. DNA binding by the archaeal histone HMf results in positive supercoiling. Proc Natl Acad Sci U S A. 1991;88(23):10397-401.
Musgrave, D. R., Sandman, K. M., & Reeve, J. N. (1991). DNA binding by the archaeal histone HMf results in positive supercoiling. Proceedings of the National Academy of Sciences of the United States of America, 88(23), 10397-401.
Musgrave DR, Sandman KM, Reeve JN. DNA Binding By the Archaeal Histone HMf Results in Positive Supercoiling. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10397-401. PubMed PMID: 1660135.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA binding by the archaeal histone HMf results in positive supercoiling. AU - Musgrave,D R, AU - Sandman,K M, AU - Reeve,J N, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 10397 EP - 401 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 88 IS - 23 N2 - HMf, a histone from the hyperthermophilic archaeon Methanothermus fervidus binds double-stranded DNA molecules in vitro, forming compact structures that visibly resemble eukaryal nucleosomes. We show here that HMf binding increases the helical periodicity of DNA molecules to approximately 11 base pairs (bp) per turn and that DNA molecules in these nucleosome-like structures are constrained in positive toroidal supercoils. Based on the mass of HMf needed to cause a change in linking number (delta Lk), the maximum delta Lk introduced into circular DNA molecules of known sizes, and electron microscopy, we estimate that each HMf-DNA structure contains between 90 and 150 bp of DNA wrapped in 1.5 positive toroidal supercoils around a core of four HMf molecules. A model and pathway for the formation of these structures in vitro are presented and the possible role of positive toroidal wrapping of the M. fervidus genome in vivo is discussed. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1660135/DNA_binding_by_the_archaeal_histone_HMf_results_in_positive_supercoiling_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1660135 DB - PRIME DP - Unbound Medicine ER -