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DNA looping between the origin of replication of Epstein-Barr virus and its enhancer site: stabilization of an origin complex with Epstein-Barr nuclear antigen 1.
Proc Natl Acad Sci U S A. 1991 Dec 01; 88(23):10870-4.PN

Abstract

Epstein-Barr nuclear antigen 1 (EBNA-1) is the only viral protein required to support replication of Epstein-Barr virus during the latent phase of its life cycle. The DNA segment required for latent replication, oriP, contains two essential binding regions for EBNA-1, termed FR and DS, that are separated by 1 kilobase pair. The FR site appears to function as a replicational enhancer providing for the start of replication at the DS site. We have used electron microscopy to visualize the interaction of EBNA-1 with its binding sites and to study the mechanism for communication between the FR and DS sites. We have found that DNA-bound EBNA-1 forms a DNA loop between the FR and DS sites. From these results, we suggest that EBNA-1 bound to the replicational enhancer acts by a DNA-looping mechanism to facilitate the initiation of DNA replication. Occupancy of the DS site alone is highly sensitive to competition with nonspecific DNA. In contrast, occupancy of the DS site by looping from FR is largely resistant to the competitor DNA. These experiments support the concept that enhancers act in cis from nearby sites to provide a high local concentration of regulatory proteins at their target sites and to stabilize regulatory interactions.

Authors+Show Affiliations

Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1660153

Citation

Su, W, et al. "DNA Looping Between the Origin of Replication of Epstein-Barr Virus and Its Enhancer Site: Stabilization of an Origin Complex With Epstein-Barr Nuclear Antigen 1." Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 23, 1991, pp. 10870-4.
Su W, Middleton T, Sugden B, et al. DNA looping between the origin of replication of Epstein-Barr virus and its enhancer site: stabilization of an origin complex with Epstein-Barr nuclear antigen 1. Proc Natl Acad Sci U S A. 1991;88(23):10870-4.
Su, W., Middleton, T., Sugden, B., & Echols, H. (1991). DNA looping between the origin of replication of Epstein-Barr virus and its enhancer site: stabilization of an origin complex with Epstein-Barr nuclear antigen 1. Proceedings of the National Academy of Sciences of the United States of America, 88(23), 10870-4.
Su W, et al. DNA Looping Between the Origin of Replication of Epstein-Barr Virus and Its Enhancer Site: Stabilization of an Origin Complex With Epstein-Barr Nuclear Antigen 1. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10870-4. PubMed PMID: 1660153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA looping between the origin of replication of Epstein-Barr virus and its enhancer site: stabilization of an origin complex with Epstein-Barr nuclear antigen 1. AU - Su,W, AU - Middleton,T, AU - Sugden,B, AU - Echols,H, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 10870 EP - 4 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 88 IS - 23 N2 - Epstein-Barr nuclear antigen 1 (EBNA-1) is the only viral protein required to support replication of Epstein-Barr virus during the latent phase of its life cycle. The DNA segment required for latent replication, oriP, contains two essential binding regions for EBNA-1, termed FR and DS, that are separated by 1 kilobase pair. The FR site appears to function as a replicational enhancer providing for the start of replication at the DS site. We have used electron microscopy to visualize the interaction of EBNA-1 with its binding sites and to study the mechanism for communication between the FR and DS sites. We have found that DNA-bound EBNA-1 forms a DNA loop between the FR and DS sites. From these results, we suggest that EBNA-1 bound to the replicational enhancer acts by a DNA-looping mechanism to facilitate the initiation of DNA replication. Occupancy of the DS site alone is highly sensitive to competition with nonspecific DNA. In contrast, occupancy of the DS site by looping from FR is largely resistant to the competitor DNA. These experiments support the concept that enhancers act in cis from nearby sites to provide a high local concentration of regulatory proteins at their target sites and to stabilize regulatory interactions. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1660153/DNA_looping_between_the_origin_of_replication_of_Epstein_Barr_virus_and_its_enhancer_site:_stabilization_of_an_origin_complex_with_Epstein_Barr_nuclear_antigen_1_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1660153 DB - PRIME DP - Unbound Medicine ER -