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Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus.
Proc Natl Acad Sci U S A. 1991 Dec 01; 88(23):10875-9.PN

Abstract

Epstein-Barr virus-encoded nuclear antigen 1 (EBNA-1) binds and activates the viral latent origin of DNA replication, oriP. We have used electron microscopy to examine the assembly of EBNA-1 onto oriP. The oriP region consists of two essential elements separated by approximately 1 kilobase pair of DNA. One element contains 20 tandom EBNA-1 binding sites [called the family of repeats (FR)] and serves to activate initiation of replication at the dyad symmetry (DS) element, which contains 4 EBNA-1 binding sites. Titration of homogeneous EBNA-1 produced in baculovirus (bEBNA-1) onto oriP DNA showed an order to the assembly of bEBNA-1 onto oriP. At low concentrations, bEBNA-1 was located exclusively on the FR element. As the level of bEBNA-1 was raised, a loop between the FR and DS elements became the most prevalent DNA-protein complex. These data suggest protein-mediated DNA looping may play a role in activating latent-phase replication of the Epstein-Barr virus.

Authors+Show Affiliations

Howard Hughes Medical Institute, Microbiology Department, Cornell University Medical College, New York, NY 10021.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

1660154

Citation

Frappier, L, and M O'Donnell. "Epstein-Barr Nuclear Antigen 1 Mediates a DNA Loop Within the Latent Replication Origin of Epstein-Barr Virus." Proceedings of the National Academy of Sciences of the United States of America, vol. 88, no. 23, 1991, pp. 10875-9.
Frappier L, O'Donnell M. Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus. Proc Natl Acad Sci U S A. 1991;88(23):10875-9.
Frappier, L., & O'Donnell, M. (1991). Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus. Proceedings of the National Academy of Sciences of the United States of America, 88(23), 10875-9.
Frappier L, O'Donnell M. Epstein-Barr Nuclear Antigen 1 Mediates a DNA Loop Within the Latent Replication Origin of Epstein-Barr Virus. Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10875-9. PubMed PMID: 1660154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Epstein-Barr nuclear antigen 1 mediates a DNA loop within the latent replication origin of Epstein-Barr virus. AU - Frappier,L, AU - O'Donnell,M, PY - 1991/12/1/pubmed PY - 1991/12/1/medline PY - 1991/12/1/entrez SP - 10875 EP - 9 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 88 IS - 23 N2 - Epstein-Barr virus-encoded nuclear antigen 1 (EBNA-1) binds and activates the viral latent origin of DNA replication, oriP. We have used electron microscopy to examine the assembly of EBNA-1 onto oriP. The oriP region consists of two essential elements separated by approximately 1 kilobase pair of DNA. One element contains 20 tandom EBNA-1 binding sites [called the family of repeats (FR)] and serves to activate initiation of replication at the dyad symmetry (DS) element, which contains 4 EBNA-1 binding sites. Titration of homogeneous EBNA-1 produced in baculovirus (bEBNA-1) onto oriP DNA showed an order to the assembly of bEBNA-1 onto oriP. At low concentrations, bEBNA-1 was located exclusively on the FR element. As the level of bEBNA-1 was raised, a loop between the FR and DS elements became the most prevalent DNA-protein complex. These data suggest protein-mediated DNA looping may play a role in activating latent-phase replication of the Epstein-Barr virus. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/1660154/Epstein_Barr_nuclear_antigen_1_mediates_a_DNA_loop_within_the_latent_replication_origin_of_Epstein_Barr_virus_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=1660154 DB - PRIME DP - Unbound Medicine ER -