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Rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method for qualitative and quantitative analysis of virgin olive oil phenolic metabolites in human low-density lipoproteins.
J Chromatogr A. 2006 May 26; 1116(1-2):69-75.JC

Abstract

A rapid method for detection and quantification of metabolites of specific olive oil phenolic compounds (hydroxytyrosol monoglucuronide, hydroxytyrosol monosulfate, tyrosol glucuronide, tyrosol sulfate and homovanillic acid sulfate) in low-density lipoprotein (LDL) fractions by solid-phase extraction (SPE) and high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) is described. A 3 microm particle size fast C18 Luna column, 5 cm x 2.0 mm I.D., was used at a flow rate of 0.6 mL/min with a mobile phase consisting of 0.1% (v/v) formic acid (A) and acetonitrile (B). A linear gradient profile was used for separation at column temperature 40 degrees C. The proposed chromatographic procedure is rapid without loosing its separation efficiency and sensitivity. Validation proofs were carried out for the method described, showing a linear system (r>0.99) and a recovery of 81.9 and 101.3% for hydroxytyrosol and homovanillic acid, respectively. The results show that this method is effective and can be used in routine analysis.

Authors+Show Affiliations

Departamento de Nutrició i Bromatologia, Centre de Referència en Tecnologia dels Aliments (CeRTA), Facultat de Farmàcia, Universitat de Barcelona. Avda. Joan XXIII s/n, Barcelona 08028, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

16603168

Citation

de la Torre-Carbot, Karina, et al. "Rapid High-performance Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry Method for Qualitative and Quantitative Analysis of Virgin Olive Oil Phenolic Metabolites in Human Low-density Lipoproteins." Journal of Chromatography. A, vol. 1116, no. 1-2, 2006, pp. 69-75.
de la Torre-Carbot K, Jauregui O, Castellote AI, et al. Rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method for qualitative and quantitative analysis of virgin olive oil phenolic metabolites in human low-density lipoproteins. J Chromatogr A. 2006;1116(1-2):69-75.
de la Torre-Carbot, K., Jauregui, O., Castellote, A. I., Lamuela-Raventós, R. M., Covas, M. I., Casals, I., & López-Sabater, M. C. (2006). Rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method for qualitative and quantitative analysis of virgin olive oil phenolic metabolites in human low-density lipoproteins. Journal of Chromatography. A, 1116(1-2), 69-75.
de la Torre-Carbot K, et al. Rapid High-performance Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry Method for Qualitative and Quantitative Analysis of Virgin Olive Oil Phenolic Metabolites in Human Low-density Lipoproteins. J Chromatogr A. 2006 May 26;1116(1-2):69-75. PubMed PMID: 16603168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method for qualitative and quantitative analysis of virgin olive oil phenolic metabolites in human low-density lipoproteins. AU - de la Torre-Carbot,Karina, AU - Jauregui,Olga, AU - Castellote,Ana I, AU - Lamuela-Raventós,Rosa M, AU - Covas,María-Isabel, AU - Casals,Isidre, AU - López-Sabater,M Carmen, Y1 - 2006/04/17/ PY - 2006/01/12/received PY - 2006/03/03/revised PY - 2006/03/07/accepted PY - 2006/4/11/pubmed PY - 2006/7/6/medline PY - 2006/4/11/entrez SP - 69 EP - 75 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1116 IS - 1-2 N2 - A rapid method for detection and quantification of metabolites of specific olive oil phenolic compounds (hydroxytyrosol monoglucuronide, hydroxytyrosol monosulfate, tyrosol glucuronide, tyrosol sulfate and homovanillic acid sulfate) in low-density lipoprotein (LDL) fractions by solid-phase extraction (SPE) and high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) is described. A 3 microm particle size fast C18 Luna column, 5 cm x 2.0 mm I.D., was used at a flow rate of 0.6 mL/min with a mobile phase consisting of 0.1% (v/v) formic acid (A) and acetonitrile (B). A linear gradient profile was used for separation at column temperature 40 degrees C. The proposed chromatographic procedure is rapid without loosing its separation efficiency and sensitivity. Validation proofs were carried out for the method described, showing a linear system (r>0.99) and a recovery of 81.9 and 101.3% for hydroxytyrosol and homovanillic acid, respectively. The results show that this method is effective and can be used in routine analysis. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/16603168/Rapid_high_performance_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_method_for_qualitative_and_quantitative_analysis_of_virgin_olive_oil_phenolic_metabolites_in_human_low_density_lipoproteins_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(06)00575-9 DB - PRIME DP - Unbound Medicine ER -